|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||SK-N-BE(2)|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: SK-N-BE(2) (ECACC 95011815)|
|Keywords:||Human Caucasian neuroblastoma|
|Cell Line Description:||Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.|
|Tissue of Origin:||neural|
|CellType:||Epitheilioid & neuroblastoid|
|Karyotype:||Modal no. 44|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.|
|Culture Medium:||EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham's F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).|
|Depositor:||Dr R A Ross and Dr BA Spengler, Department of Biological Sciences, Fordham University, NY|
|References:||Cancer Res 1978;38:3751; Life Sciences 1991;50:7; Science 1976;191:185; J Natl Cancer Inst 1976;57:683|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Please confirm your country of origin from the list below.