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UK Health Security Agency

DMS 153

DMS 153

Catalogue No.

95062827

Cell Line Name

DMS 153

Cell Line Description

DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH), bombesin (BN), 17 beta-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.

Characteristics

Receptors

Bombesin, Epidermal growth factor (EGF)

Tissue of Origin

Lung (liver metastasis)

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10
D5S818: 12
D7S820: 8,10
D13S317: 13
D16S539: 12,13
TH01: 7,9.3
TPOX: 11
vWA: 14,15

Karyotype

Modal no. 41-42

Applications

Human lung tumour model

Disease

Small Cell Cancer

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.Cells grow in large clumps.

Culture Medium

Waymouth's MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Growth Mode

Semi-adherent aggregates

Additional Info

Depositor

Prof OS Pettengill and Dr G D Sorenson, Dartmouth Medical School, Lebanon, NH

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cancer 1980;45:906; Cancer 1981;47:1289; Regulatory Peptides 1982;4:59

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: DMS 153 (ECACC 95062827).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.