General Cell Collection Detail
ECACC General Cell Collection: Hepa-1c1c7
| Supplied by: | European Collection of Authenticated Cell Cultures (ECACC) |
|---|---|
| Culture Type: | Cell line |
| Collection: | ECACC General Collection |
| Catalogue No.: | 95090613 |
| Cell Line Name: | Hepa-1c1c7 |
| Other Collection No.: | ATCC CRL 2026 |
| Citation Guidance: | If use of this culture results in a scientific publication, it should be cited in the publication as: Hepa-1c1c7 (ECACC 95090613) |
| Keywords: | Mouse hepatoma |
| Cell Line Description: | Hepa-1c1c7 was cloned from the cell line Hepa-1cl which was obtained from Hepa-1. Hepa-1 was derived from the BW7756 hepatoma that developed in a C57L mouse. Hepa-1c1c7 cells express the aryl hydrocarbon (Ah) receptor and were shown to be highly inducible for cytochrome P450IA1. A variety of mutants from this cell line have been established. |
| Species: | Mouse |
| Tissue of Origin: | hepatoma |
| CellType: | Epithelial |
| Growth Mode: | Adherent |
| Karyotype: | Not specified |
| Biosafety Information: |
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK) All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises. Hyperlinks to MSDS documents: Frozen cell cultures Material Safety Data Sheet Growing cell cultures Material Safety Data Sheet Nucleic acids derived from cell cultures Material Safety Data Sheet |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Culture Medium: | Alpha MEM without nucleosides + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Depositor: | Obtained from ATCC |
| Originator: | No |
| Country: | USA |
| References: | Proc Natl Acad Sci, USA 1979;76:373; Biochemie 1991;73:61 |
| Additional Bibliography: | Not specified |
| Patents: | None specified by Depositor |
| Release Conditions: | No |
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Product DocumentsThe Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Available Formats
Frozen
Growing
DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)
cDNA-20µl
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Frozen
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Growing
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DNA-5µg (100ng/µl)
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RNA-5µg (100ng/µl)
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cDNA-20µl
This product is only available through your local distributor.
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