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UK Health Security Agency

Tufted Deer

Tufted Deer

Catalogue No.

96011147

Cell Line Name

Tufted Deer

Cell Line Description

The fibroblast-like cell line named Tufted Deer has been derived from the lung of a male deer. Important Note - Species Query for the cell line ‘Tufted Deer': ECACC uses DNA barcoding to verify the species of cell lines. Using the Basic Local Alignment Search Tool (BLAST) DNA sequence alignment program the cytochrome C oxidase subunit I (cox1) gene sequence for the Tufted Deer (Elaphodus cephalophus) cell line has a match of 92% with Père David's Deer (Elaphurus davidianus) and a match of 88% with Tufted Deer. To assign a species the Quality Control criteria at ECACC requires a DNA barcode match of 95% or higher. ECACC has discussed this result with colleagues at the National Center for Biotechnology Information (NCBI) who host BLAST and Barcode of Life Database (BOLD) Systems. The conclusion is that the cell line named Tufted Deer is Deer but not Tufted Deer as originally described when deposited with ECACC. The exact species of Deer is not known. It should be noted however, that search engines such as BLAST allow open access and the sequences placed into them are not reviewed. This means the accuracy of the data in the databases the Tufted Deer sequence has been compared to cannot be guaranteed.

Characteristics

Tissue of Origin

Lung

Karyotype

48XY

Disease

None Stated

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C Culture attachment appears to be surface dependent, ECACC recommends the use of Corning Plastics.

Culture Medium

RPMI 1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Prof L Rui-Qing, Kunming Cell Bank, Kunming

Country of Origin

China

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Zoological Research 1984;5(1):71

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: Tufted Deer (ECACC 96011147).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.