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UK Health Security Agency

SNU-C2B

SNU-C2B

Catalogue No.

96020932

Cell Line Name

SNU-C2B

Cell Line Description

This cell line was derived from a fourth passage nude mouse xenograft of tumour fragments obtained from a 43 year old Asian female patient prior to therapy. The cells have a high concentration of receptors for EGF. This cell line has lost both chromosomes 8 and 11. This is one of 14 colorectal carcinoma cell lines derived by JG Park and associates during the years 1982 to 1985. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously.

Characteristics

Tissue of Origin

Cecum

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,14
D5S818: 8,9
D7S820: 12,13
D13S317: 9,10
D16S539: 8,9
TH01: 9
TPOX: 8
vWA: 15,19

Karyotype

Pseudodiploid. Modal chromosome number is 46 at 70% with polyploidy at 16.4%. The del(3) (p23), t(4,?), t(8;?) (p22), del (9) (p13), del (11) (p13), t(1;8) (p13;p24), t(1;?) (q11,?) and 2 other marker chromosomes, if any, occur only sporadically.

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial with loosely associated aggregates of cells

Subculture Routine

Split sub-confluent cultures (70-80 %) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 37 C; 5% CO₂.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.0mM sodium pyruvate

Growth Mode

Semi-adherent aggregates

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Park JG, Oie HK, Sugarbaker PH, Henslee JG, Chen TR, Johnson BE, Gazdar A: Characteristics of cell lines established from human colorectal carcinoma. Cancer Res 1987, 47(24 Pt 1):6710-6718.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: SNU-C2B (ECACC 96020932).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.