Culture Collections

General Cell Collection Detail

ECACC General Cell Collection: SNU-C2B

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 96020932
Cell Line Name: SNU-C2B
Other Collection No.: ATCC CCL-250
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: SNU-C2B (ECACC 96020932)
Keywords: Human Colorectal Carcinoma
Cell Line Description: This cell line was derived from a fourth passage nude mouse xenograft of tumour fragments obtained from a 43 year old Asian female patient prior to therapy. The cells have a high concentration of receptors for EGF. This cell line has lost both chromosomes 8 and 11. This is one of 14 colorectal carcinoma cell lines derived by JG Park and associates during the years 1982 to 1985. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously.
Species: Human
Tissue of Origin: Cecum
CellType: Epithelial with loosely associated aggregates of cells
Growth Mode: Semi-adherent aggregates
DNA Profile: STR-PCR Data:

Amelogenin: X
CSF1PO: 11,14
D13S317: 9,10
D16S539: 8,9
D5S818: 8,9
D7S820: 12,13
THO1: 9
vWA: 15,19

Karyotype: Pseudodiploid. Modal chromosome number is 46 at 70% with polyploidy at 16.4%. The del(3) (p23), t(4,?), t(8;?) (p22), del (9) (p13), del (11) (p13), t(1;8) (p13;p24), t(1;?) (q11,?) and 2 other marker chromosomes, if any, occur only sporadically.
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80 %) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37 C; 5% CO2.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.0mM sodium pyruvate
Depositor: Obtained from ATCC
Originator: No
Country: USA
References: Park JG, Oie HK, Sugarbaker PH, Henslee JG, Chen TR, Johnson BE, Gazdar A: Characteristics of cell lines established from human colorectal carcinoma. Cancer Res 1987, 47(24 Pt 1):6710-6718.
Additional Bibliography: Not specified
Patents: None specified by Depositor
Release Conditions: No

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)

  • Frozen
  • Growing
  • DNA-5µg (100ng/µl)
  • RNA-5µg (100ng/µl)
  • cDNA-20µl

This product is only available through your local distributor.

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