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UK Health Security Agency

J.CaM1.6

J.CaM1.6

Catalogue No.

96060401

Cell Line Name

J.CaM1.6

Cell Line Description

The T cell leukaemia line J.CaM1.6 was derived from the parent cell line Jurkat E.6-1 (ECACC catalogue no. 88042803). Jurkat cells were mutagenised with ethyl methanesulfonate and subsequently grown in phytohemagglutinin containing media for 2 weeks. J.CaM1.6 express a structurally normal but functionally defective CD3/Ti complex. The cells fail to exhibit inositolphospholipid metabolism or Ca²+ mobilisation in response to anti-CD3 or anti-Ti monoclonal antibodies. They display resistance to anti-CD3 induced apoptosis, constitutively express Fas and are sensitive to anti-Fas induced apoptosis. It was shown that J.CaM1.6 cells are defective in early signalling events that follow TCR stimulation owing to loss of lck tyrosine kinase function due to the lack of exon 7 in the lck transcript.

Characteristics

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11
D5S818: 9
D7S820: 8,10
D13S317: 8,12
D16S539: 10,11
TH01: 6,9.3
TPOX: 8,10
vWA: 18,19

Karyotype

Not specified

Applications

Study of apoptosis, signal transduction receptor analysis

Disease

Lymphoblastic Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

To keep the cells in exponential growth, maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37°C; 5 - 7% CO₂. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days. .

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Proc Natl Acad Sci, USA 1987;84:6879; Cell 1992;70:585; Biochem Biophys Res Comm 1995;213:994

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: J.CaM1.6 (ECACC 96060401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.