Culture Collections

General Cell Collection Detail

ECACC General Cell Collection: 293-PERV-PK-CIRCE

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 97051411
Cell Line Name: 293-PERV-PK-CIRCE
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: 293-PERV-PK-CIRCE (ECACC 97051411)
Keywords: Human embryo kidney, infected with endogenous retrovirus of pigs
Cell Line Description: 293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (ECACC catalogue no. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.
Species: Human
Tissue of Origin: Kidney
Growth Mode: Adherent aggregates
Karyotype: Not specified
GMO Status: Genetically Modified Organism Class 1 (GMO1)
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO2 3
Culture Medium: DMEM + 2mM Glutamine + 10% HI-FBS
Depositor: Dr C Patience, Institute of Cancer Research, Chester Beatty Laboratories, London, UK
Originator: Yes
Country: UK
References: Nature Medicine 1997 3:282
Additional Bibliography: Not specified
Patents: None specified by Depositor
Release Conditions: No

product documentsProduct Documents

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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