|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC HLA-Typed Collection|
|Cell Line Name:||JY|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: JY (ECACC 94022533)|
|Keywords:||Human HLA-Typed Lymphoblastoid Cell Line|
|Cell Line Description:||An Epstein-Barr virus (EBV) transformed lymphoblastoid cell line with Human Leukocyte Antigen (HLA)-typing data available.|
|Tissue of Origin:||Blood|
|GMO Status:||Genetically Modified Organism Class 1 (GMO1)|
|Genetic Modification:||This is an Epstein-Barr Virus (EBV) transformed cell line|
|Hazard Group (ACDP):||1|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||When resuscitating from frozen, cells should be seeded at 0.5 x 106 cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 105 cells/ml. Maintain cultures between 3-9 x 105 cells/ml for maximum growth; 5% CO2; 37°C. Cells form floating aggregates of varying size. Protocol: resuscitation of frozen cells|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). To help establish a culture the concentration of FBS can be increased to 20%.|
|Release Conditions:||Frozen and growing culture can only be supplied to academic and not for profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.|
|*Information as provided at time of deposit of the cell line into the ECACC HLA-Typed Collection.|
|Serological Profile: HLA Class I & Class II Antigens|
|HLA DR:||4 13|
|HLA DQ:||1 3|
|DNA Profile: HLA Class I & Class II Antigens|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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