Culture Collections

Virus LENTICULE® Discs - Microbiological Reference Material for Internal Quality Control

LENTICULE discs - Blue HOMEPAGE

 

Applications:

  • as qualitative or quantitative control strains for routine food, water and environmental testing
  • for method development and validations
  • for use in research studies
  • for training and educational purposes

 

In partnership with the Centre for Environment, Fisheries & Aquaculture Science (Cefas), PHE provides reference materials (RMs) for norovirus (genogroups I and II) and hepatitis A virus.

The discs consist of viruses in a solid water-soluble matrix that is stable for at least one year from manufacture, easy to use and suitable for a wide range of applications. The LENTICULE discs must be stored at -20°C ± 5°C, although short periods at ambient temperature such as during transportation, does not affect viability. All LENTICULE discs are supplied with a minimum expiry date of three months from date of purchase. Each pack of 10 LENTICULE discs comes with easy-to-follow instructions; all orders will be supplied from a single batch.

 

Catalogue number    Reference Material Genome copies per LENTICULE disc range
RM000HAV Hepatitis A virus (HAV)

2.1x103-3.2x104

RMNOROG1 Norovirus GI

1.4x103-6.8x102

RMNOROG2 Norovirus GII

6.5x103-1.3x105

The RMs are supplied with a certificate of analysis which gives reference values. These values are generated using a real time RT-PCR using Cefas’ ISO 17025 accredited procedures for virus quantification based upon ISO 15216-1 (Method for Quantification). Reference values are generated using a real time RT-PCR using Cefas’ ISO 17025 accredited procedures for virus quantification based on ISO 15216-1 (Method for Quantification).

The NCTC and NCPF LENTICULE discs are now produced by Merck (previously Sigma-Aldrich). Please visit their website to search the product listings.

 

Introduction to reference materials for norovirus and hepatitis A virus

 

Handling Information
Instructions for Use – Norovirus and Hepatitis A virus LENTICULE discs
 
Safety Information
Material Safety Data Sheet – Norovirus LENTICULE discs
Material Safety Data Sheet – Hepatitis A virus LENTICULE discs

 

Import Permits

For delivery to the USA a CDC import permit is required. For delivery to Canada an import permit from Health Canada is required. 

 

LENTICULE is a trade mark of Public Health England. The method of preparation and the LENTICULE discs themselves are subject to the International Patent Application published as WO 01/05941.

 

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.