DNA barcoding will determine the species of a cell line. It involves amplifying and sequencing mitochondrial DNA. Mitochondrial DNA shows 5–10-fold more differences between species than nuclear DNA. The genetic analysis of mitochondrial genome sequences is simpler than the analysis of nuclear DNA because only a single PCR and sequencing reaction are normally required; mitochondrial DNA shows only very low within-species variation.
|Authentication method & sample numbers||Price|
Animal cell line authentication (species identification) by DNA barcoding
£95 per sample
Please complete the two forms below and send to us using the address below prior to arranging shipment of samples:
Once the forms have been received, reviewed and accepted by ECACC, you will be issued with a reference number that will need to be quoted on your samples and in all future correspondence. You will also need to provide a purchase order number.
If sending DNA:
If sending growing cultures:
If sending frozen cultures:
If sending PBS washed cell pellets:
Flasks and ampoules should be adequately packed and transported according to UN602 regulations. If you use a courier service to ship your samples, please contact the company involved for advice on their own approved packaging. ECACC will be happy to return any packaging to you for re-use.
Parcels should be addressed to:
QC Testing Department Culture Collections Public Health England Porton Down Salisbury SP4 0JG UK
Notification on dispatch of samples to ECACC is advised, please email us on firstname.lastname@example.org
Please confirm your country of origin from the list below.