Culture Collections

Mycoplasma testing

 Mycoplasma_1

ECACC can test your cell lines, cell culture media and reagents to determine if mycoplasma is present. Many researchers choose to use our service to ensure they are working with mycoplasma free cell cultures.  The effects of mycoplasma contamination on cell lines can be wide ranging and are often underestimated. These include:

  • alteration of growth rate
  • induction of morphological changes
  • chromosomal aberrations
  • altered cell metabolism
  • decreased viability upon resuscitation of frozen ampoule.

All laboratories carrying out cell culture should test for mycoplasma contamination on a regular basis. We recommend that a combination of methods is used to determine whether a sample is free of mycoplasma.  This is because the different methods have different levels of detection sensitivity and some strains of mycoplasma do not grow in vitro. These can be detected by DNA stain and PCR.


Three different mycoplasma detection methods are routinely used by our scientists:

  1. PCR
  2. Indirect Hoechst Stain
  3. Culture Isolation

 

Mycoplasma detection methods and prices 

Name of Test

Catalogue Number

Price

Sensitivity

Time Taken

Species Range

Meets FDA points to consider in   characterisation of cell lines used to produce biologicals (1993)

PCR

ECSR33

£72.50

Low/   Medium

1 day

Majority

No

Indirect Hoechst Stain

ECSR60

£125

Medium

2-3 days

All

Yes

Culture Isolation

ECSR61

£175

High

3-4 weeks

Majority

Yes

All three Mycoplasma detection methods

ECSR39

£300

High

3-4 weeks

All

See above for each test

Sample preparation from frozen ampoule 

 ECSR30

 £75

 N/A

 

N/A

 

 N/A

 

N/A

 

 

 

 

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