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Bacteria Collection: Legionella pneumophila

NCTC Number: NCTC 11233
Current Name: Legionella pneumophila
Original Strain Reference: Los Angeles-1
Other Collection No: ATCC 33156; LOS ANGELES-1, WDCM 00180, CIP103858
Previous Catalogue Name: Legionella pneumophila
Type Strain: No
Family: Legionellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: buff charcoal yeast ext + co2,37, carbon dioxide required condl:nutrient
16S rRNA Gene Sequence: >gb|AF000656|LOS ANGELES-1| ATCC33156|Legionella pneumophila strain ATCC33156 16S-23S rRNA intergenicregion.| atcctcctcggctcc...
23S rRNA Gene Sequence: >gb|Z30439|LOS ANGELES-1|ATCC 33156|L.pneumophila Serogroup 4 Los Angeles-1 genes for 23S ribosomal RNAand 5S ribosomal RNA.| gcctccctcaagatg... >gb|Z24708|LOS ANGELES-1|ATCC 33156|L.pneumophila 23S and 5S rRNA genes, partial CDSs.| gcctccctcaagatg... >gb|AF000656|LOS ANGELES-1| ATCC33156|Legionella pneumophila strain ATCC33156 16S-23S rRNA intergenicregion.| atcctcctcggctcc... >gb|Z24708|LOS ANGELES-1|ATCC 33156|L.pneumophila 23S and 5S rRNA genes, partial CDS's.| gcctccctcaagatg...
Extended Bibliography: showhide Show bibliography
Ref #: 95520
Author(s): Riffard,S.;Lo Presti,F.;Normand,P.;Forey,F.;Reyrolle,M.;Etienne,J.;Vandenesch,F.
Journal: Int J Syst Bacteriol
Title: Species identification of Legionella via intergenic 16S-23S ribosomal spacer PCR analysis
Volume: 48 Pt 3
Page(s): 723-30
Year: 1998
Keyword(s): GENBANK/AF000654 GENBANK/AF000655 GENBANK/AF000656 Base Sequence Legionella/*classification/genetics Molecular Sequence Data Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics
Remarks: Species identification of Legionella in routine laboratory testing is hampered by the lack of highly discriminatory phenotypic tests. Amplification polymorphism of the intergenic 16S-23S spacer regions (ISR) has been previously developed for identification of species within the Legionellaceae [Hookey, J.V., Birtles, R.J. & Saunders, N.A. (1995). J Clin Microbiol 33, 2377-2381], but it did not provide enough resolution to distinguish all members of the bluish-white autofluorescent species and the red autofluorescent group of the Legionellaceae. By choosing new primers that target regions 4 (positions 1521-1541 of Escherichia coli 16S rRNA gene) and 6 (positions 114-132 of E.coli 23S rRNA gene) within the rDNA operon close to the 16S-23S intergenic spacer, 34 profiles were determined among the 79 type and reference strains representing 42 species that were tested. Analysis of the RFLP generated after Hinfl restriction digestion of the PCR products further improved the method, allowing complete discrimination among the species and subspecies of Legionella tested. Twenty-three well-identified strains from unrelated origins belonging to seven species gave amplification patterns identical to that of their type strain. The technique was also tested on 80 field isolates that could not be unequivocally assigned to groups by phenotypic methods. Seventy-two per cent (58/80) of these isolates had a profile identical to that of a type strain, while 27% (22/80) may correspond to new taxa since their ISR-PCR profiles did not match any of the known profiles.
URL: 9734026
Ref #: 54193
Author(s): Ko,K.S.;Lee,H.K.;Park,M.Y.;Park,M.S.;Lee,K.H.;Woo,S.Y.;Yun,Y.J.;Kook,Y.H.
Journal: J Bacteriol
Title: Population genetic structure of Legionella pneumophila inferred from RNA polymerase gene (rpoB) and DotA gene (dotA) sequences
Volume: 184
Page(s): 2123-30
Year: 2002
Keyword(s): Amino Acid Sequence *Bacterial Proteins Base Sequence Carrier Proteins/*genetics DNA-Directed RNA Polymerases/*genetics Legionella pneumophila/*classification/genetics Membrane Proteins/*genetics Molecular Sequence Data Serotyping
Remarks: The population structure of Legionella pneumophila was studied by using partial RNA polymerase gene (rpoB) and DotA gene (dotA) sequences. Trees inferred from rpoB sequences showed that two subspecies of L. pneumophila, Legionella pneumophila subsp. pneumophila and Legionella pneumophila subsp. fraseri, were clearly separated genetically. In both rpoB and dotA trees, 79 Korean isolates used in this study constituted six clonal populations, four of which (designated subgroups P-I to P-IV) were identified in L. pneumophila subsp. pneumophila and two of which (designated subgroups F-I and F-II) were identified in L. pneumophila subsp. fraseri. Although the relationships among subgroups were not identical, such subgrouping was congruent between the rpoB and dotA trees. Type strains of several serogroups did not belong to any subgroup, presumably because isolates similar to these strains were not present among our local sample of the population. There was evidence that horizontal gene transfer or recombination had occurred within L. pneumophila. Contrary to the phylogeny from rpoB and the taxonomic context, subgroups P-III and P-IV of L. pneumophila subsp. pneumophila proved to be closely related to those of L. pneumophila subsp. fraseri or showed a distinct clustering in the dotA tree. It can be inferred that dotA of subgroups P-III and P-IV has been transferred horizontally from other subspecies. The diverse distribution of serogroup 1 strains through the gene trees suggests that surface antigen-coding genes that determine serogroup can be exchanged. Thus, it can be inferred that genetic recombination has been important in the evolution of L. pneumophila.
URL: 11914343
Ref #: 95439
Author(s): Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A.
Journal: FEMS Microbiol Lett
Title: Species-specific detection of Legionella using polymerase chain reaction and reverse dot-blotting
Volume: 140
Page(s): 111-9
Year: 1996
Keyword(s): GENBANK/Z30431 GENBANK/Z30432 GENBANK/Z30433 GENBANK/Z30434 GENBANK/Z30435 GENBANK/Z30436 GENBANK/Z30437 GENBANK/Z30438 GENBANK/Z30439 GENBANK/Z30440 GENBANK/Z30441 GENBANK/Z30442 GENBANK/Z30443 GENBANK/Z30444 GENBANK/Z30445 GENBANK/Z30446 GENBANK/Z30447 GENBANK/Z30448 GENBANK/Z30449 GENBANK/Z30450 GENBANK/Z30451 GENBANK/Z30452 GENBANK/Z30453 GENBANK/Z30454 GENBANK/Z30455 GENBANK/Z30534 GENBANK/Z30535 GENBANK/Z30536 GENBANK/Z30537 GENBANK/Z30538 etc. Base Sequence DNA Primers/genetics DNA Probes/genetics DNA, Bacterial/*genetics/isolation & purification DNA, Ribosomal/genetics Humans Legionella/classification/*genetics/isolation & purification Legionnaires' Disease/diagnosis/microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks: Legionella pneumophila and some other Legionella species are capable of causing Legionnaire's disease, a potentially fatal pneumonia. The identification of legionellae by standard laboratory techniques such as culture is difficult and time consuming. In the present work, the DNA sequence of the 23S-5S spacer region was determined for 43 Legionella isolates, and the sequence information was used to develop a species-specific detection system using PCR and reverse dot-blotting which employs just one PCR amplicon to perform genus- and species-specific detection. L. pneumophila serogroups 1-16 as well as 21 non-pneumophila isolates could be identified and differentiated at the species level using this system.
URL: 8764471
Ref #: 13685
Author(s): Robinson,P.N.;Heidrich,B.;Tiecke,F.;Fehrenbach,F.J.;Rolfs,A.
Journal: FEMS Microbiol Lett
Title: Species-specific detection of Legionella using polymerase chain reaction
Volume: 140
Page(s): 111-119
Year: 1996
Keyword(s): 0 (DNA Primers) 0 (DNA Probes) 0 (DNA, Bacterial) 0 (DNA, Ribosomal) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 23S) 0 (RNA, Ribosomal, 5S) Base Sequence Comparative Study DNA Primers/genetics DNA Probes/genetics DNA, Bacterial/*genetics/isolation & purification DNA, Ribosomal/genetics Human Legionella/classification/*genetics/isolation & purification Legionnaires' Disease/diagnosis/microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics RNA, Ribosomal, 5S/genetics Sequence Homology, Nucleic Acid Species Specificity
Remarks: Legionella pneumophila and some other Legionella species are capable of
URL: 96291654
Data: (ATCC 33156) R. E. Weaver, CDC Atlanta in 1978 / Serogroup 4 / subsp. fraseri / Fry, N. K. et al. (1991) J. gen. Microbiol. 137, 1215
Accession Date: 01/01/1978
History: WEAVER R E, CDC ATLANTA, GEORGIA
Authority: Brenner et al. 1979 (AL)
Depositor: WEAVER R E
Taxonomy: TaxLink: S1650 (Legionella pneumophila subsp. pneumophila Brenner et al. 1979) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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