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Bacteria Collection: Mobiluncus curtisii subsp. holmesii

NCTC Number: NCTC 11657
Current Name: Mobiluncus curtisii subsp. holmesii
Original Strain Reference: BV376-6
Other Collection No: ATCC 35242; BV 376-6
Previous Catalogue Name: Mobiluncus curtisii subsp. holmesii
Type Strain: Yes
Family: Actinomycetaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 48 hours, 37°C, anaerobic
Conditions for growth on liquid media: nutrient broth,37, strict anaerobe
Isolated From: human, vaginal discharge
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1324128
16S rRNA Gene Sequence: >gb|X86006|ATCC 35242|M.curtisii 16S rRNA gene (ssp. holmesii).| gaagaagcgccggct... >gb|X82604|ATCC 35242|M.curtisii 16S rRNA gene (ssp. holmesii).| tgacatacactgcga...
Extended Bibliography: showhide Show bibliography
Ref #: 58580
Author(s): Tiveljung,A.;Backstrom,J.;Forsum,U.;Monstein,H.J.
Journal: APMIS
Title: Broad-range PCR amplification and DNA sequence analysis reveals variable motifs in 16S rRNA genes of Mobiluncus species
Volume: 103
Page(s): 755-63
Year: 1996
Keyword(s): GENBANK/X82602 GENBANK/X82603 GENBANK/X82604 GENBANK/X82605 GENBANK/X82606 GENBANK/X82607 GENBANK/X82608 GENBANK/X82609 GENBANK/X82610 GENBANK/X82611 GENBANK/X86004 GENBANK/X86005 GENBANK/X86006 Base Sequence DNA, Ribosomal/*genetics Mobiluncus/*genetics Molecular Sequence Data RNA, Ribosomal, 16S/*genetics Sequence Alignment Sequence Analysis
Remarks: Using DNA primers based on highly conserved regions of bacterial 16S ribosomal RNA genes, a technique was established for detection of Mobiluncus species by polymerase chain reaction (PCR) and hybridization analysis. Part of the 16S rRNA genes of Mobiluncus mulieris, Mobiluncus curtisii and uncharacterized Mobiluncus strains were analyzed by broad-range PCR amplification and direct DNA sequencing analysis. Sequence comparison of the partial 16S rRNA genes of Mobiluncus curtisii, Mobiluncus mulieris and atypical Mobiluncus strains studied indicated genus and species-specific motifs within the variable regions V3, V4 and V9 of 16S ribosomal DNAs. A Mobiluncus curtisii-specific primer, located within the variable region V3 of the 16S rRNA gene, was designed for Southern blot hybridization analysis of broad-range PCR products. Broad-range amplification combined with a M. curtisii-specific hybridization probe, Mob V3, distinguished between Mobiluncus curtisii, Mobiluncus mulieris, and atypical Mobiluncus strains.
URL: 8534436
Ref #: 58579
Author(s): Tiveljung,A.;Forsum,U.;Monstein,H.J.
Journal: Int J Syst Bacteriol
Title: Classification of the genus Mobiluncus based on comparative partial 16S rRNA gene analysis
Volume: 46
Page(s): 332-6
Year: 1996
Keyword(s): GENBANK/X82602 GENBANK/X82603 GENBANK/X82604 GENBANK/X82605 GENBANK/X82606 GENBANK/X82607 GENBANK/X82608 GENBANK/X86004 GENBANK/X86005 GENBANK/X86006 Base Sequence Blotting, Southern DNA, Bacterial/genetics Genes, Bacterial Mobiluncus/*classification/genetics Molecular Sequence Data RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Homology, Nucleic Acid
Remarks: On the basis of partial 16S rRNA gene sequences and the results of Southern blot analyses, we confirmed the division of the genus Mobiluncus into the species Mobiluncus curtisii and Mobiluncus mulieris. Division of M. curtisii into M. curtisii subsp. curtisii and M. curtisii subsp. holmesii was not supported by our data.
URL: 8573515
Ref #: 13726
Author(s): Tiveljung,A.;Backstrom,J.;Forsum,U.;Monstein,H.J.
Journal: APMIS
Title: Broad-range PCR amplification and DNA sequence analysis reveals variable motifs in 16S rRNA genes of Mobiluncus species
Volume: 103
Page(s): 755-63
Year: 1996
Keyword(s): GENBANK/X82602 GENBANK/X82603 GENBANK/X82604 GENBANK/X82605 GENBANK/X82606 GENBANK/X82607 GENBANK/X82608 GENBANK/X82609 GENBANK/X82610 GENBANK/X82611 GENBANK/X86004 GENBANK/X86005 GENBANK/X86006 Base Sequence DNA, Ribosomal/*genetics Mobiluncus/*genetics Molecular Sequence Data RNA, Ribosomal, 16S/*genetics Sequence Alignment Sequence Analysis Support, Non-U.S. Gov't
Remarks: Using DNA primers based on highly conserved regions of bacterial 16S ribosomal RNA genes, a technique was established for detection of Mobiluncus species by polymerase chain reaction (PCR) and hybridization analysis. Part of the 16S rRNA genes of Mobiluncus mulieris, Mobiluncus curtisii and uncharacterized Mobiluncus strains were analyzed by broad-range PCR amplification and direct DNA sequencing analysis. Sequence comparison of the partial 16S rRNA genes of Mobiluncus curtisii, Mobiluncus mulieris and atypical Mobiluncus strains studied indicated genus and species-specific motifs within the variable regions V3, V4 and V9 of 16S ribosomal DNAs. A Mobiluncus curtisii-specific primer, located within the variable region V3 of the 16S rRNA gene, was designed for Southern blot hybridization analysis of broad-range PCR products. Broad-range amplification combined with a M. curtisii-specific hybridization probe, Mob V3, distinguished between Mobiluncus curtisii, Mobiluncus mulieris, and atypical Mobiluncus strains.
URL: 96125706
Ref #: 13725
Author(s): Tiveljung,A.;Forsum,U.;Monstein,H.J.
Journal: Int J Syst Bacteriol
Title: Classification of the genus Mobiluncus based on comparative partial 16S rRNA gene analysis
Volume: 46
Page(s): 332-6
Year: 1996
Keyword(s): GENBANK/X82602 GENBANK/X82603 GENBANK/X82604 GENBANK/X82605 GENBANK/X82606 GENBANK/X82607 GENBANK/X82608 GENBANK/X86004 GENBANK/X86005 GENBANK/X86006 Base Sequence Blotting, Southern Comparative Study DNA, Bacterial/genetics Genes, Bacterial Mobiluncus/*classification/genetics Molecular Sequence Data RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't
Remarks: On the basis of partial 16S rRNA gene sequences and the results of Southern blot analyses, we confirmed the division of the genus Mobiluncus into the species Mobiluncus curtisii and Mobiluncus mulieris. Division of M. curtisii into M. curtisii subsp. curtisii and M. curtisii subsp. holmesii was not supported by our data.
URL: 96138996
Data: (ATCC 35242) Type strain / C. A. Spiegel, Medical College of Wisconsin, USA / Vaginal discharge / Short type (see also Falcivibrio)
Accession Date: 01/01/1984
History: MEDICAL COLLEGE OF WISCONSIN ,USA
Authority: Spiegel and Roberts 1984 (ALI)
Depositor: SPIEGEL C A
Taxonomy: TaxLink: S1909 (Mobiluncus curtisii subsp. curtisii Spiegel and Roberts 1984) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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