Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Leclercia adecarboxylata

NCTC Number: NCTC 13032
Current Name: Leclercia adecarboxylata
Other Collection No: ATCC 23216
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS473439
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
Miscellaneous Sequence Data: >gb|AY370853|ATCC 23216|Leclercia adecarboxylata strain ATCC 23216 gyrase B (gyrB) gene,partial cds.| aaagtatccggcggc...
Extended Bibliography: showhide Show bibliography
Ref #: 43181
Author(s): Delmas,J.;Breysse,F.;Devulder,G.;Flandrois,J.P.;Chomarat,M.
Journal: Diagn Microbiol Infect Dis
Title: Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene
Volume: 55
Page(s): 263-8
Year: 2006
Keyword(s): Bacterial Typing Techniques/*methods DNA Gyrase/*genetics Enterobacteriaceae/enzymology/genetics/*isolation & purification Genotype Humans Sequence Analysis, DNA/methods
Remarks: Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMerieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.
URL: 16626902
Accession Date: 14/06/2007
Taxonomy: TaxLink: S1632 (Leclercia adecarboxylata (leclerc 1962) tamura et al. 1987) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

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Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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Available Formats

Ampoule (Bacteria)

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