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Bacteria Collection: Brevibacillus brevis

NCTC Number: NCTC 2611
Current Name: Brevibacillus brevis
Original Strain Reference: Ford 27B
Other Collection No: ATCC 8246; CCM 2050; DSM 30; FORD 27B; JCM 2503; LMG 16703; N R SMITH 604; NCIB 9372; PORTER
Previous Catalogue Name: Bacillus brevis
Type Strain: Yes
Family: Paenibacillaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 30°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1178935
16S rRNA Gene Sequence: >gb|AB101593|DSM30T|Brevibacillus brevis gene for 16S rRNA, partial sequence.| cgaacgctggcggcg... >gb|AF478094|ATCC 8246|Brevibacillus brevis clone JG-1F 16S ribosomal RNA gene, and16S-23S internal transcribed spacer, partial sequence.| atgccgcggtgaata... >gb|D78457|JCM2503|Brevibacillus brevis DNA for 16S ribosomal RNA.| gagagtttgatcctg...
23S rRNA Gene Sequence: >gb|AF478094|ATCC 8246|Brevibacillus brevis clone JG-1F 16S ribosomal RNA gene, and16S-23S internal transcribed spacer, partial sequence.| atgccgcggtgaata...
Bibliography: SMITH N R ET AL 1952 USDA AGRT MONOGR 16 119;FORD W W 1916 J BACT 1
Extended Bibliography: showhide Show bibliography
Ref #: 20136
Author(s): Shida,O.;Takagi,H.;Kadowaki,K.;Komagata,K.
Journal: Int J Syst Bacteriol
Title: Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov
Volume: 46
Page(s): 939-46
Year: 1996
Keyword(s): GENBANK/D78454 GENBANK/D78455 GENBANK/D78456 GENBANK/D78457 GENBANK/D78458 GENBANK/D78459 GENBANK/D78460 GENBANK/D78461 GENBANK/D78462 GENBANK/D78463 GENBANK/D78464 Bacillus/*classification/genetics Base Sequence DNA, Ribosomal/chemistry Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics
Remarks: 16S rRNA gene sequences of the type strains of 11 species belonging to the Bacillus brevis and Bacillus aneurinolyticus groups were determined. On the basis of the results of gene sequence analyses, these species were separated into two clusters. The B. brevis cluster included 10 species, namely, Bacillus brevis, Bacillus agri, Bacillus centrosporus, Bacillus choshinensis, Bacillus parabrevis, Bacillus reuszeri, Bacillus formosus, Bacillus borstelensis, Bacillus laterosporus, and Bacillus thermoruber. Bacillus aneurinolyticus and Bacillus migulanus belonged to the B. aneurinolyticus cluster. Moreover, the two clusters were phylogenetically distinct from other Bacillus, Amphibacillus, Sporolactobacillus, Paenibacillus, and Alicyclobacillus species. On the basis of our data, we propose reclassification of the B. brevis cluster as Brevibacillus gen. nov. and reclassification of the B. aneurinolyticus cluster as Aneurinibacillus gen. nov. By using 16S rRNA gene sequence alignments, two specific PCR amplification primers were designed for differentiating the two new genera from each other and from other aerobic, endospore-forming organisms.
URL: 8863420
Ref #: 22093
Author(s): Xu,D.;Cote,J.C.
Journal: Int J Syst Evol Microbiol
Title: Phylogenetic relationships between Bacillus species and related genera inferred from comparison of 3' end 16S rDNA and 5' end 16S-23S ITS nucleotide sequences
Volume: 53
Page(s): 695-704
Year: 2003
Keyword(s): GENBANK/AF478062 GENBANK/AF478063 GENBANK/AF478064 GENBANK/AF478065 GENBANK/AF478066 GENBANK/AF478067 GENBANK/AF478068 GENBANK/AF478069 GENBANK/AF478070 GENBANK/AF478071 GENBANK/AF478072 GENBANK/AF478073 GENBANK/AF478074 GENBANK/AF478075 GENBANK/AF478076 GENBANK/AF478077 GENBANK/AF478078 GENBANK/AF478079 GENBANK/AF478080 GENBANK/AF478081 GENBANK/AF478082 GENBANK/AF478083 GENBANK/AF478084 GENBANK/AF478085 GENBANK/AF478086 GENBANK/AF478087 GENBANK/AF478088 GENBANK/AF478089 GENBANK/AF478090 GENBANK/AF478091 GENBANK/AF478092 GENBANK/AF478093 GENBANK/AF478094 GENBANK/AF478095 GENBANK/AF478096 GENBANK/AF478097 GENBANK/AF478098 GENBANK/AF478099 GENBANK/AF478100 GENBANK/AF478101 GENBANK/AF478102 GENBANK/AF478103 GENBANK/AF478104 GENBANK/AF478105 GENBANK/AF478106 GENBANK/AF478107 GENBANK/AF478108 GENBANK/AF478109 GENBANK/AF478110 GENBANK/AF478111 3' Untranslated Regions/*genetics 5' Untranslated Regions/*genetics Bacillus/*classification/genetics DNA, Ribosomal/analysis DNA, Ribosomal Spacer/*analysis Genes, rRNA Molecular Sequence Data *Phylogeny RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/genetics *Sequence Analysis, DNA Species Specificity
Remarks: The nucleotide sequences of the 3' end of the 16S rDNA and the 16S-23S internal transcribed spacer (ITS) of 40 Bacillaceae species were determined. These included 21 Bacillus, 9 Paenibacillus, 6 Brevibacillus, 2 Geobacillus, 1 Marinibacillus and 1 Virgibacillus species. Comparative sequence analysis of a 220 bp region covering a highly conserved 150 bp sequence located at the 3' end of the 16S rRNA coding region and a conserved 70 bp sequence located at the 5' end of the 16S-23S ITS of the 40 species and six sequences available in GenBank were used to infer the phylogenetic relationships between all 46 taxa. When a maximal distance (D(max), where D refers to the number of nucleotide substitutions per site) of 0.31 was introduced as a threshold to determine groupings, 10 phylogenetically distinct clusters were revealed. Twenty-six Bacillus species were separated in seven groups (I, II, III, IV, V, VI and X), but Bacillus circulans remained ungrouped. All six Brevibacillus species under study were in Group VII. The nine Paenibacillus species fell into two distinct groups (VIII and IX). Species with D(max) values within 0.05 were considered to be very closely related. These were Bacillus psychrophilus and Bacillus psychrosaccharolyticus in Group II; 'Bacillus maroccanus' and Bacillus simplex in Group II; Bacillus amyloliquefaciens, Bacillus atrophaeus, Bacillus mojavensis and Bacillus subtilis in Group VI; Bacillus fusiformis and Bacillus sphaericus in Group VI; Brevibacillus brevis and Brevibacillus formosus in Group VII; Paenibacillus gordonae and Paenibacillus validus in Group VIII; and Bacillus anthracis, Bacillus cereus, Bacillus mycoides and Bacillus thuringiensis in Group X. The phylogenetic classification presented here is, in general, in agreement with current classifications based on phenotypic and molecular data. Our findings suggest, however, that in some cases, further divisions or, conversely, further groupings might be warranted. Should current classifications be re-examined in the light of our results, D(max) values of 0.31 and 0.05, as exemplified here, may prove useful threshold values for the grouping of Bacillaceae into taxa akin to genera and species, respectively. These D(max) thresholds may also reveal, in a different way, bacterial species for which further characterization might be warranted for proper classification and/or reassignment.
URL: 12807189
Ref #: 12125
Author(s): Shida,O.;Takagi,H.;Kadowaki,K.;Komagata,K.
Journal: Int J Syst Bacteriol
Title: Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov
Volume: 46
Page(s): 939-46
Year: 1996
Keyword(s): GENBANK/D78454 GENBANK/D78455 GENBANK/D78456 GENBANK/D78457 GENBANK/D78458 GENBANK/D78459 GENBANK/D78460 GENBANK/D78461 GENBANK/D78462 GENBANK/D78463 GENBANK/D78464 Bacillus/*classification/genetics Base Sequence DNA, Ribosomal/chemistry Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics
Remarks: 16S rRNA gene sequences of the type strains of 11 species belonging to the Bacillus brevis and Bacillus aneurinolyticus groups were determined. On the basis of the results of gene sequence analyses, these species were separated into two clusters. The B. brevis cluster included 10 species, namely, Bacillus brevis, Bacillus agri, Bacillus centrosporus, Bacillus choshinensis, Bacillus parabrevis, Bacillus reuszeri, Bacillus formosus, Bacillus borstelensis, Bacillus laterosporus, and Bacillus thermoruber. Bacillus aneurinolyticus and Bacillus migulanus belonged to the B. aneurinolyticus cluster. Moreover, the two clusters were phylogenetically distinct from other Bacillus, Amphibacillus, Sporolactobacillus, Paenibacillus, and Alicyclobacillus species. On the basis of our data, we propose reclassification of the B. brevis cluster as Brevibacillus gen. nov. and reclassification of the B. aneurinolyticus cluster as Aneurinibacillus gen. nov. By using 16S rRNA gene sequence alignments, two specific PCR amplification primers were designed for differentiating the two new genera from each other and from other aerobic, endospore-forming organisms.
URL: 97016794
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 3768
Author(s): Fahmy,F.;Flossdorf,J.;Claus,D.
Journal: System. Appl. Microbiol.
Title: The DNA base composition of the type strains of the genus Bacillus.
Volume: 6
Page(s): 60-65
Year: 1985
Ref #: 4238
Author(s): Nakamura,L.K.
Journal: Int. J. Syst. Bacteriol.
Title: Bacillus brevis Migula 1900 taxonomy: reassociation and base composition of DNA.
Volume: 41
Page(s): 510-515
Year: 1991
Ref #: 5252
Author(s): Shida,O.;Takagi,H.;Kadowaki,K.;Udaka,S.;Nakamura,L.K.;Komagata,K.
Journal: Int. J. Syst. Bacteriol.
Title: Proposal of Bacillus reuszeri sp. nov., Bacillus formosus sp. nov., nom. rev., and Bacillus borstelensis, sp. nov., nom. rev.
Volume: 45
Page(s): 93-100
Year: 1995
Ref #: 6615
Author(s): Heyndrickx,M.;Vandermeulebroecke,K.;Kersters,K.;DeVos,P.;Logan,N.A.;Aziz,M.A.;Ali,N.;Berkeley,R.C.W.
Journal: Int. J. Syst. Bacteriol.
Title: A polyphasic reassessment of the genus Paenibacillus, reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb. nov., and emended description of
Volume: 46
Page(s): 988-1003
Year: 1996
Ref #: 6650
Author(s): Shida,O.;Takagi,H.;Kadowaki,K.;Komagata,K.
Journal: Int. J. Syst. Bacteriol.
Title: Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov.
Volume: 46
Page(s): 939-946
Year: 1996
Data: (N. R. Smith 604, Porter, ATCC 8246, NCIB 9372) Type strain / T. Gibson, Edinburgh in 1963 / Ford, W. W. (1916) J. Bact. 1, 522 / Smith, N. et al. (1952) USDA Agr. Monogr. No. 16, 119 / Smith, N. R. et al. (1964) J. gen. Microbiol. 34, 269
Accession Date: 01/01/1963
History: ISOLATED BY FORD W W,1916
Authority: (Migula 1900) Shida et al. 1996
Depositor: GIBSON T
Taxonomy: TaxLink: S393 (Bacillus brevis) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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