Culture Collections

Bacteria and Mycoplasmas detail

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Bacteria Collection: Staphylococcus haemolyticus

NCTC Number: NCTC 11042
Current Name: Staphylococcus haemolyticus
Original Strain Reference: CCM 2737
Other Collection No: ATCC 29970; CCM 2737; DSM 20263; SM 131
Previous Catalogue Name: Staphylococcus haemolyticus
Type Strain: Yes
Family: Staphylococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, human skin
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS654924
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
16S rRNA Gene Sequence: >gb|X66100|CCM2737|S.haemolyticus gene for 16S ribosomal RNA.| agagtttgattnngg... >gb|Z26896|DSM 20263|S.haemolyticus gene for 16S ribosomal RNA.| ctcaggatgaacgct... >gb|AY688060|DSM 20263|Staphylococcus haemolyticus strain DSM 20263 16S ribosomal RNAgene, partial sequence.| catgcaagtcgagcg...
23S rRNA Gene Sequence: >gb|AF146772|NCTC11042|Staphylococcus haemolyticus NCTC11042 23S ribosomal RNA gene,partial sequence.| tcagaaggcagaccc...
Bibliography: SCHLEIFER K H & KLOOS W E 1975 INT J SYST BACT 25 50
Extended Bibliography: showhide Show bibliography
Ref #: 95508
Author(s): Anthony,R.M.;Brown,T.J.;French,G.L.
Journal: J Clin Microbiol
Title: Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array
Volume: 38
Page(s): 781-8
Year: 2000
Keyword(s): GENBANK/AF146762 GENBANK/AF146763 GENBANK/AF146764 GENBANK/AF146765 GENBANK/AF146766 GENBANK/AF146768 GENBANK/AF146769 GENBANK/AF146770 GENBANK/AF146771 GENBANK/AF146772 GENBANK/AF146773 GENBANK/AF146774 Bacteremia/*diagnosis/microbiology Bacteria/classification/*genetics/isolation & purification DNA Primers DNA, Bacterial/analysis DNA, Ribosomal/analysis Humans Molecular Sequence Data *Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction/*methods Predictive Value of Tests RNA, Ribosomal, 23S/*genetics
Remarks: The rapid identification of bacteria in blood cultures and other clinical specimens is important for patient management and antimicrobial therapy. We describe a rapid (<4 h) detection and identification system that uses universal PCR primers to amplify a variable region of bacterial 23S ribosomal DNA, followed by reverse hybridization of the products to a panel of oligonucleotides. This procedure was successful in discriminating a range of bacteria in pure cultures. When this procedure was applied directly to 158 unselected positive blood culture broths on the day when growth was detected, 125 (79.7%) were correctly identified, including 4 with mixed cultures. Nine (7.2%) yielded bacteria for which no oligonucleotide targets were present in the oligonucleotide panel, and 16 culture-positive broths (10.3%) produced no PCR product. In seven of the remaining eight broths, streptococci were identified but not subsequently grown, and one isolate of Staphylococcus aureus was misidentified as a coagulase-negative staphylococcus. The accuracy, range, and discriminatory power of the assay can be continually extended by adding further oligonucleotides to the panel without significantly increasing complexity or cost.
URL: 10655385
Ref #: 81199
Author(s): Becker,K.;Harmsen,D.;Mellmann,A.;Meier,C.;Schumann,P.;Peters,G.;von Eiff,C.
Journal: J Clin Microbiol
Title: Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species
Volume: 42
Page(s): 4988-95
Year: 2004
Keyword(s): GENBANK/AY688029 GENBANK/AY688030 GENBANK/AY688031 GENBANK/AY688032 GENBANK/AY688033 GENBANK/AY688034 GENBANK/AY688035 GENBANK/AY688036 GENBANK/AY688037 GENBANK/AY688038 GENBANK/AY688039 GENBANK/AY688040 GENBANK/AY688041 GENBANK/AY688042 GENBANK/AY688043 GENBANK/AY688044 GENBANK/AY688045 GENBANK/AY688046 GENBANK/AY688047 GENBANK/AY688048 GENBANK/AY688049 GENBANK/AY688050 GENBANK/AY688051 GENBANK/AY688052 GENBANK/AY688053 GENBANK/AY688054 GENBANK/AY688055 GENBANK/AY688056 GENBANK/AY688057 GENBANK/AY688058 GENBANK/AY688059 GENBANK/AY688060 GENBANK/AY688061 GENBANK/AY688062 GENBANK/AY688063 GENBANK/AY688064 GENBANK/AY688065 GENBANK/AY688066 GENBANK/AY688067 GENBANK/AY688068 GENBANK/AY688069 GENBANK/AY688070 GENBANK/AY688071 GENBANK/AY688072 GENBANK/AY688073 GENBANK/AY688074 GENBANK/AY688075 GENBANK/AY688076 GENBANK/AY688077 GENBANK/AY688078 GENBANK/AY688079 GENBANK/AY688080 GENBANK/AY688081 GENBANK/AY688082 GENBANK/AY688083 GENBANK/AY688084 GENBANK/AY688085 GENBANK/AY688086 GENBANK/AY688087 GENBANK/AY688088 GENBANK/AY688089 GENBANK/AY688090 GENBANK/AY688091 GENBANK/AY688092 GENBANK/AY688093 GENBANK/AY688094 GENBANK/AY688095 GENBANK/AY688096 GENBANK/AY688097 GENBANK/AY688098 GENBANK/AY688099 GENBANK/AY688100 GENBANK/AY688101 GENBANK/AY688102 GENBANK/AY688103 GENBANK/AY688104 GENBANK/AY688105 GENBANK/AY688106 GENBANK/AY688107 GENBANK/AY688108 GENBANK/AY688109 *Bacterial Typing Techniques DNA, Bacterial/analysis DNA, Ribosomal/analysis *Databases, Nucleic Acid Genes, rRNA Humans Molecular Sequence Data RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Species Specificity Staphylococcal Infections/*microbiology Staphylococcus/*classification/genetics/metabolism
Remarks: To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal sequences and choosing a reporting criterion of > or =98.7% similarity for a "distinct species," a statistical error probability of 1.0% was calculated. To evaluate this database, a 16S rDNA fragment (corresponding to Escherichia coli positions 54 to 510) of 55 clinical Staphylococcus isolates (including those of the small-colony variant phenotype) were sequenced and analyzed by the RIDOM approach. Of these isolates, 54 (98.2%) had a similarity score above the proposed threshold using RIDOM; 48 (87.3%) of the sequences gave a perfect match, whereas 83.6% were found by searching National Center for Biotechnology Information (NCBI) database entries. In contrast to RIDOM, which showed four ambiguities at the species level (mainly concerning Staphylococcus intermedius versus Staphylococcus delphini), the NCBI database search yielded 18 taxon-related ambiguities and showed numerous matches exhibiting redundant or unspecified entries. Comparing molecular results with those of biochemical procedures, ID 32 Staph (bioMerieux, Marcy I'Etoile, France) and VITEK 2 (bioMerieux) failed to identify 13 (23.6%) and 19 (34.5%) isolates, respectively, due to incorrect identification and/or categorization below acceptable values. In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants.
URL: 15528685
Ref #: 13707
Author(s): Anthony,R.M.;Brown,T.J.;French,G.L.
Journal: J Clin Microbiol
Title: Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array
Volume: 38
Page(s): 781-8
Year: 2000
Keyword(s): GENBANK/AF146762 GENBANK/AF146763 GENBANK/AF146764 GENBANK/AF146765 GENBANK/AF146766 GENBANK/AF146768 GENBANK/AF146769 GENBANK/AF146770 GENBANK/AF146771 GENBANK/AF146772 GENBANK/AF146773 GENBANK/AF146774 Bacteremia/*diagnosis/microbiology Bacteria/classification/*genetics/isolation & purification DNA Primers DNA, Bacterial/analysis DNA, Ribosomal/analysis Human Molecular Sequence Data *Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction/*methods Predictive Value of Tests RNA, Ribosomal, 23S/*genetics
Remarks: The rapid identification of bacteria in blood cultures and other clinical specimens is important for patient management and antimicrobial therapy. We describe a rapid (<4 h) detection and identification system that uses universal PCR primers to amplify a variable region of bacterial 23S ribosomal DNA, followed by reverse hybridization of the products to a panel of oligonucleotides. This procedure was successful in discriminating a range of bacteria in pure cultures. When this procedure was applied directly to 158 unselected positive blood culture broths on the day when growth was detected, 125 (79.7%) were correctly identified, including 4 with mixed cultures. Nine (7.2%) yielded bacteria for which no oligonucleotide targets were present in the oligonucleotide panel, and 16 culture-positive broths (10.3%) produced no PCR product. In seven of the remaining eight broths, streptococci were identified but not subsequently grown, and one isolate of Staphylococcus aureus was misidentified as a coagulase-negative staphylococcus. The accuracy, range, and discriminatory power of the assay can be continually extended by adding further oligonucleotides to the panel without significantly increasing complexity or cost.
URL: 20122462
Data: (SM 131, DSM 20263) Type strain / CCM, Czechoslovakia in 1976 / Human skin / Schleifer, K. H. & Kloos, W. E. (1975) Int. J. syst. Bact. 25, 50
Accession Date: 01/01/1976
History: ISOLATED BY KLOOS W E 1975 - CCM 1976
Authority: Scheifer and Kloos 1975
Depositor: CZECH. COLL OF MICROORGANISMS
Taxonomy: TaxLink: S2784 (Staphylococcus epidermidis (Winslow and Winslow 1908) Evans 1916) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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