Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Vibrio fluvialis

NCTC Number: NCTC 11327
Current Name: Vibrio fluvialis
Original Strain Reference: VL 5125
Other Collection No: ATCC 33809; VL 5125; WDCM 00137
Previous Catalogue Name: Vibrio fluvialis
Type Strain: Yes
Family: Vibrionaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group f
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,30, facultative anaerobe
Isolated From: faeces;faeces from patient with diarrhoea
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS846864
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
16S rRNA Gene Sequence: >gb|X76335|NCTC 11327 T|V.fluvialis (NCTC 11327 T) 16S rRNA gene.| agagtttgatnntgg...
Bibliography: LEE J ET AL 1981 J APPL BACT 50 73 94; LEE J ET AL 1978 INT J SYST BACT 28
Extended Bibliography: showhide Show bibliography
Ref #: 55417
Author(s): Aznar,R.;Ludwig,W.;Amann,R.I.;Schleifer,K.H.
Journal: Int J Syst Bacteriol
Title: Sequence determination of rRNA genes of pathogenic Vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probes
Volume: 44
Page(s): 330-7
Year: 1994
Keyword(s): GENBANK/X76333 GENBANK/X76334 GENBANK/X76335 GENBANK/X76336 GENBANK/X76337 Bacterial Typing Techniques Base Sequence DNA, Ribosomal/*genetics Genes, Bacterial/*genetics Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Phylogeny RNA, Ribosomal/*genetics/isolation & purification RNA, Ribosomal, 16S/genetics/isolation & purification RNA, Ribosomal, 23S/genetics/isolation & purification Vibrio/classification/*genetics/*isolation & purification/pathogenicity Virulence
Remarks: A comparative analysis of seven new 16S rRNA gene sequences of pathogenic Vibrio species with previously published vibrio sequences confirmed that Vibrio vulnificus represents a group that is not closely related to the core organisms of the genus Vibrio. In addition, we found that V. vulnificus, Listonella (Vibrio) anguillarum and Vibrio diazotrophicus branch off separately from the core group. A comparison of the 16S rRNA gene sequences of V. vulnificus strains belonging to biotypes 1 and 2 revealed that the sequences of all but four biotype 1 strains were identical to each other but slightly different (17 bases) from the sequences of the rest of the V. vulnificus strains investigated. In addition, the sequences of variable regions of the 23S rRNA genes of Vibrio fluvialis, Vibrio furnissii, Vibrio harveyi, Vibrio cholerae, and V. vulnificus C7184 and TW1 were determined, aligned, and compared with all available bacterial 23S rRNA sequences in order to search for specific target sites. As a result, four oligonucleotide probes specific for V. vulnificus were synthesized, and the specificities of these probes were evaluated by dot blot hybridization to membrane-bound RNAs from 21 V. vulnificus strains, 13 strains belonging to other Vibrio species, 61 strains belonging to species that are members of the alpha, beta, and gamma subclasses of the Proteobacteria, and 3 eucaryotic microorganisms. Two probes hybridized with all of the V. vulnificus strains tested, and the other two probes distinguished V. vulnificus biotype 1 strains from all other organisms. In situ identification of V. vulnificus by using tetramethylrhodamine- or fluorescein-labelled oligonucleotides is now possible.
URL: 8186099
Ref #: 13618
Author(s): Aznar,R.;Ludwig,W.;Amann,R.I.;Schleifer,K.H.
Journal: Int J Syst Bacteriol
Title: Sequence determination of rRNA genes of pathogenic Vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probes
Volume: 44
Page(s): 330-7
Year: 1994
Keyword(s): GENBANK/X76333 GENBANK/X76334 GENBANK/X76335 GENBANK/X76336 GENBANK/X76337 Bacterial Typing Techniques Base Sequence Comparative Study DNA, Ribosomal/*genetics Genes, Bacterial/*genetics Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Phylogeny RNA, Ribosomal/*genetics/isolation & purification RNA, Ribosomal, 16S/genetics/isolation & purification RNA, Ribosomal, 23S/genetics/isolation & purification Support, Non-U.S. Gov't Vibrio/classification/*genetics/*isolation & purification/pathogenicity Virulence
Remarks: A comparative analysis of seven new 16S rRNA gene sequences of pathogenic Vibrio species with previously published vibrio sequences confirmed that Vibrio vulnificus represents a group that is not closely related to the core organisms of the genus Vibrio. In addition, we found that V. vulnificus, Listonella (Vibrio) anguillarum and Vibrio diazotrophicus branch off separately from the core group. A comparison of the 16S rRNA gene sequences of V. vulnificus strains belonging to biotypes 1 and 2 revealed that the sequences of all but four biotype 1 strains were identical to each other but slightly different (17 bases) from the sequences of the rest of the V. vulnificus strains investigated. In addition, the sequences of variable regions of the 23S rRNA genes of Vibrio fluvialis, Vibrio furnissii, Vibrio harveyi, Vibrio cholerae, and V. vulnificus C7184 and TW1 were determined, aligned, and compared with all available bacterial 23S rRNA sequences in order to search for specific target sites. As a result, four oligonucleotide probes specific for V. vulnificus were synthesized, and the specificities of these probes were evaluated by dot blot hybridization to membrane-bound RNAs from 21 V. vulnificus strains, 13 strains belonging to other Vibrio species, 61 strains belonging to species that are members of the alpha, beta, and gamma subclasses of the Proteobacteria, and 3 eucaryotic microorganisms. Two probes hybridized with all of the V. vulnificus strains tested, and the other two probes distinguished V. vulnificus biotype 1 strains from all other organisms. In situ identification of V. vulnificus by using tetramethylrhodamine- or fluorescein-labelled oligonucleotides is now possible.
URL: 94242609
Data: Type strain / J. Lee, Maidstone, 1979 / I. Hug, Dacca / Faeces, patient with diarrhoea / Biovar 1, Group F Vibrio / Lee, J. et al. (1978) Int. J. syst. Bact. 28, 99 / Lee, J. et al. (1981) J. appl. Bact. 50, 73
Accession Date: 01/01/1979
History: ISOLATED BY HUG I 1976 - LEE J V PHLS MAIDSTONE
Authority: Lee et al. 1981
Depositor: LEE J
Taxonomy: TaxLink: S3547 (Vibrio fluvialis Lee et al. 1981) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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