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Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Micrococcus luteus

NCTC Number: NCTC 2665
Current Name: Micrococcus luteus
Original Strain Reference: 2665
Other Collection No: ATCC 15307; ATCC 4698; CCM 169; CN 3475; DSM 20030; NCIB 9278; WDCM 00111
Previous Catalogue Name: Micrococcus luteus
Type Strain: Yes
Family: Micrococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 30°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1054576
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AJ536198|TYPE STRAIN: DSM 20030|Micrococcus luteus 16S rRNA gene, type strain DSM 20030T.| ggctcaggatgaacg... >gb|AF542073|ATCC 4698|Micrococcus luteus 16S ribosomal RNA gene, partial sequence.| cgatgaagcccagct...
23S rRNA Gene Sequence: >gb|X06484 S44491|DSM 20030|Micrococcus luteus 23S rRNA gene.| gtgaaagtttccaag...
Bibliography: FLEMING A 1929 LANCET 1 217
Extended Bibliography: showhide Show bibliography
Ref #: 95459
Author(s): Regensburger,A.;Ludwig,W.;Frank,R.;Blocker,H.;Schleifer,K.H.
Journal: Nucleic Acids Res
Title: Complete nucleotide sequence of a 23S ribosomal RNA gene from Micrococcus luteus
Volume: 16
Page(s): 2344
Year: 1988
Keyword(s): GENBANK/X06484 Base Sequence DNA, Ribosomal/*genetics Micrococcus/*genetics Molecular Sequence Data RNA, Ribosomal/*genetics RNA, Ribosomal, 23S/*genetics
URL: 3357787
Ref #: 95460
Author(s): Roller,C.;Ludwig,W.;Schleifer,K.H.
Journal: J Gen Microbiol
Title: Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes
Volume: 138
Page(s): 1167-75
Year: 1992
Keyword(s): GENBANK/J01695 GENBANK/K00637 GENBANK/K02663 GENBANK/L05015 GENBANK/M10606 GENBANK/M31904 GENBANK/M32365 GENBANK/M62805 GENBANK/M62806 GENBANK/M62807 GENBANK/M85086 GENBANK/M85087 GENBANK/M85088 GENBANK/M85089 GENBANK/M85090 GENBANK/M85091 GENBANK/M85092 GENBANK/M85093 GENBANK/M85094 GENBANK/X00007 GENBANK/X00343 GENBANK/X00512 GENBANK/X06458 GENBANK/X07408 GENBANK/X12612 GENBANK/X14249 GENBANK/X16368 GENBANK/X53853 GENBANK/X53854 GENBANK/X53855 Base Composition Base Sequence DNA Transposable Elements/*genetics DNA, Bacterial/*genetics DNA, Ribosomal/*genetics Gram-Positive Bacteria/classification/*genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Ribosomal, 23S/*genetics Sequence Homology, Nucleic Acid
Remarks: An insertion of about 100 bases within the central part of the 23S rRNA genes was found to be a phylogenetic marker for the bacterial line of descent of Gram-positive bacteria with a high DNA G + C content. The insertion was present in 23S rRNA genes of 64 strains representing the major phylogenetic groups of Gram-positive bacteria with a high DNA G+C content, whereas it was not found in 23S rRNA genes of 55 (eu)bacteria representing Gram-positive bacteria with a low DNA G + C content and all other known (eu)bacterial phyla. The presence of the insertion could be easily demonstrated by comparative gel electrophoretic analysis of in vitro-amplified 23S rDNA fragments, which contained the insertion. The nucleotide sequences of the amplified fragments were determined and sequence similarities of at least 44% were found. The overall similarity values are lower than those of 16S and 23S rRNA sequences of the particular organism. Northern hybridization experiments indicated the presence of the insertion within the mature 23S rRNA of Corynebacterium glutamicum.
URL: 1326592
Ref #: 13124
Author(s): Regensburger,A.;Ludwig,W.;Frank,R.;Blocker,H.;Schleifer,K.H.
Journal: Nucleic Acids Res
Title: Complete nucleotide sequence of a 23S ribosomal RNA gene from Micrococcus luteus
Volume: 16
Page(s): 2344
Year: 1988
Keyword(s): GENBANK/X06484 Base Sequence DNA, Ribosomal/*genetics Micrococcus/*genetics Molecular Sequence Data RNA, Ribosomal/*genetics RNA, Ribosomal, 23S/*genetics Support, Non-U.S. Gov't
URL: 88189834
Ref #: 13125
Author(s): Roller,C.;Ludwig,W.;Schleifer,K.H.
Journal: J Gen Microbiol
Title: Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes
Volume: 138 ( Pt 6)
Page(s): 1167-75
Year: 1992
Keyword(s): GENBANK/J01695 GENBANK/K00637 GENBANK/K02663 GENBANK/L05015 GENBANK/M10606 GENBANK/M31904 GENBANK/M32365 GENBANK/M62805 GENBANK/M62806 GENBANK/M62807 GENBANK/M85086 GENBANK/M85087 GENBANK/M85088 GENBANK/M85089 GENBANK/M85090 GENBANK/M85091 GENBANK/M85092 GENBANK/M85093 GENBANK/M85094 GENBANK/X00007 GENBANK/X00343 GENBANK/X00512 GENBANK/X06458 GENBANK/X07408 GENBANK/X12612 GENBANK/X14249 GENBANK/X16368 GENBANK/X53853 GENBANK/X53854 GENBANK/X53855 Base Composition Base Sequence Comparative Study DNA Transposable Elements/*genetics DNA, Bacterial/*genetics DNA, Ribosomal/*genetics Gram-Positive Bacteria/classification/*genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Ribosomal, 23S/*genetics Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't
Remarks: An insertion of about 100 bases within the central part of the 23S rRNA genes was found to be a phylogenetic marker for the bacterial line of descent of Gram-positive bacteria with a high DNA G + C content. The insertion was present in 23S rRNA genes of 64 strains representing the major phylogenetic groups of Gram-positive bacteria with a high DNA G+C content, whereas it was not found in 23S rRNA genes of 55 (eu)bacteria representing Gram-positive bacteria with a low DNA G + C content and all other known (eu)bacterial phyla. The presence of the insertion could be easily demonstrated by comparative gel electrophoretic analysis of in vitro-amplified 23S rDNA fragments, which contained the insertion. The nucleotide sequences of the amplified fragments were determined and sequence similarities of at least 44% were found. The overall similarity values are lower than those of 16S and 23S rRNA sequences of the particular organism. Northern hybridization experiments indicated the presence of the insertion within the mature 23S rRNA of Corynebacterium glutamicum.
URL: 92407488
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1335
Author(s): Schleifer,K.H.;Heise,W.;Meyer,S.A.
Journal: FEMS Microbiol. Lett.
Title: Deoxyribonucleic acid hybridization studies among some micrococci.
Volume: 6
Page(s): 33-36
Year: 1979
Ref #: 450
Author(s): Fleming,A.
Journal: Lancet
Title: Lysozyme: a bacteriolytic ferment found normally in tissues and secretions.
Volume: 1
Page(s): 217-220
Year: 1929
Ref #: 495
Author(s): Schleifer,K.H.;Kloos,W.E.;Moore,A.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomic status of Micrococcus luteus (Schroeter 1872) Cohn 1872: correlation between peptidoglycan type and genetic compatibility.
Volume: 22
Page(s): 224-227
Year: 1972
Ref #: 499
Author(s): Silvestri,L.G.;Hill,L.R.
Journal: J. Bacteriol.
Title: Agreement between deoxyribonucleic acid base composition and taxometric classification of Gram-positive cocci.
Volume: 90
Page(s): 136-140
Year: 1965
Data: (ATCC 15307, DSM 20030, CN 3475) Type strain / A. Fleming in 1928 / Lysozyme sensitive / Micrococcus lysodeikticus / Fleming, A. (1929) Lancet, i, 217
Accession Date: 01/01/1928
Authority: (Schroeter 1872) Cohn 1872 (AL)
Depositor: FLEMING A
Taxonomy: TaxLink: S1860 (Micrococcus luteus (Schroeter 1872) Cohn 1872 emend. Wieser et al. 2002) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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