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Bacteria Collection: Mycoplasma hyorhinis

NCTC Number: NCTC 10130
Current Name: Mycoplasma hyorhinis
Original Strain Reference: BTS 7
Other Collection No: ATCC 17981; BTS 7
Previous Catalogue Name: Mycoplasma hyorhinis
Type Strain: Yes
Family: Mycoplasmataceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Isolated From: mammal, pig nasal cavity
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1295692
16S rRNA Gene Sequence: >gb|AF294993|ATCC 17981|Mycoplasma hyorhinis 16S-23S ribosomal RNA intergenic spacer,complete sequence; and 23S ribosomal RNA gene, partial sequence.| ctttctacggagtac...
23S rRNA Gene Sequence: >gb|AF294993|ATCC 17981|Mycoplasma hyorhinis 16S-23S ribosomal RNA intergenic spacer,complete sequence; and 23S ribosomal RNA gene, partial sequence.| ctttctacggagtac...
Bibliography: SWITZER W P 143 ANN N Y ACAD SCI 1967 281;SWITZER W P 1955 AM J VET RES 16
Extended Bibliography: showhide Show bibliography
Ref #: 14410
Author(s): Kong,F.;James,G.;Gordon,S.;Zelynski,A.;Gilbert,G.L.
Journal: Appl Environ Microbiol
Title: Species-specific PCR for identification of common contaminant mollicutes in cell culture
Volume: 67
Page(s): 3195-200
Year: 2001
Keyword(s): GENBANK/AF294989 GENBANK/AF294990 GENBANK/AF294991 GENBANK/AF294992 GENBANK/AF294993 GENBANK/AF294994 GENBANK/AF294995 GENBANK/AF294996 Cell Line/*microbiology DNA Primers DNA, Ribosomal Spacer/genetics Humans Molecular Sequence Data Mollicutes/*classification/genetics/*isolation & purification Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sensitivity and Specificity Sequence Analysis, DNA Species Specificity
Remarks: Mycoplasma arginini, M. fermentans, M. hyorhinis, M. orale, and Acholeplasma laidlawii are the members of the class Mollicutes most commonly found in contaminated cell cultures. Previous studies have shown that the published PCR primer pairs designed to detect mollicutes in cell cultures are not entirely specific. The 16S rRNA gene, the 16S-23S rRNA intergenic spacer region, and the 5' end of the 23S rRNA gene, as a whole, are promising targets for design of mollicute species-specific primer pairs. We analyzed the 16S rRNA genes, the 16S-23S rRNA intergenic spacer regions, and the 5' end of the 23S rRNA genes of these mollicutes and developed PCR methods for species identification based on these regions. Using high melting temperatures, we developed a rapid-cycle PCR for detection and identification of contaminant mollicutes. Previously published, putative mollicute-specific primers amplified DNA from 73 contaminated cell lines, but the presence of mollicutes was confirmed by species-specific PCR in only 60. Sequences of the remaining 13 amplicons were identified as those of gram-positive bacterial species. Species-specific PCR primers are needed to confirm the presence of mollicutes in specimens and for identification, if required.
URL: 11425741
Ref #: 11947
Author(s): Kong,F.;James,G.;Gordon,S.;Zelynski,A.;Gilbert,G.L.
Journal: Appl Environ Microbiol
Title: Species-specific PCR for identification of common contaminant mollicutes in cell culture
Volume: 67
Page(s): 3195-200
Year: 2001
Keyword(s): GENBANK/AF294989 GENBANK/AF294990 GENBANK/AF294991 GENBANK/AF294992 GENBANK/AF294993 GENBANK/AF294994 GENBANK/AF294995 GENBANK/AF294996 Cell Line/*microbiology DNA Primers DNA, Ribosomal Spacer/genetics Human Molecular Sequence Data Mollicutes/*classification/genetics/*isolation & purification Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sensitivity and Specificity Sequence Analysis, DNA Species Specificity
Remarks: Mycoplasma arginini, M. fermentans, M. hyorhinis, M. orale, and Acholeplasma laidlawii are the members of the class Mollicutes most commonly found in contaminated cell cultures. Previous studies have shown that the published PCR primer pairs designed to detect mollicutes in cell cultures are not entirely specific. The 16S rRNA gene, the 16S-23S rRNA intergenic spacer region, and the 5' end of the 23S rRNA gene, as a whole, are promising targets for design of mollicute species-specific primer pairs. We analyzed the 16S rRNA genes, the 16S-23S rRNA intergenic spacer regions, and the 5' end of the 23S rRNA genes of these mollicutes and developed PCR methods for species identification based on these regions. Using high melting temperatures, we developed a rapid-cycle PCR for detection and identification of contaminant mollicutes. Previously published, putative mollicute-specific primers amplified DNA from 73 contaminated cell lines, but the presence of mollicutes was confirmed by species-specific PCR in only 60. Sequences of the remaining 13 amplicons were identified as those of gram-positive bacterial species. Species-specific PCR primers are needed to confirm the presence of mollicutes in specimens and for identification, if required.
URL: 21318714
Data: (ATCC 17981) Type strain / MRL Colindale in 1970 / ATCC / W. P. Switzer / Pig, nasal cavity / Switzer, W. P. (1955) Am. J. vet. Res. 16, 540 / Switzer, W. P. (1967) Ann. N. Y. Acad. Sci. 143, 281
Accession Date: 01/01/1970
History: ISOLATED BY SWITZER W P-ATCC
Authority: Switzer 1955 (AL)
Depositor: MYCOPLASMA REF LAB COLINDALE
Taxonomy: TaxLink: S2044 (Mycoplasma hyorhinis Switzer 1955) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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