Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Mycobacterium fortuitum

NCTC Number: NCTC 10394
Current Name: Mycobacterium fortuitum
Original Strain Reference: J. C. Cruz 1
Other Collection No: ATCC 6841; DSM 46621; IMET 10605; J C CRUZ 1; TMC 1529
Previous Catalogue Name: Mycobacterium fortuitum
Type Strain: Yes
Family: Mycobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 30°C, aerobic
Conditions for growth on liquid media: todd-hewitt broth,37, aerobic
Isolated From: human, cold abscess
16S rRNA Gene Sequence: >gb|AJ536039|TYPE STRAIN: DSM 46621|Mycobacterium fortuitum subsp. fortuitum 16S rRNA gene.| gacgaacgctggcgg... >gb|AJ296160|ATCC 6841|Mycobacterium fortuitum tyrS gene, 3-mag gene (partial) and 16SrRNA gene (partial), strain ATCC 6841.| ggatccgctgctcgc... >gb|U92089|ATCC6841|Mycobacterium fortuitum ATCC 6841, 16S ribosomal RNA gene, partialsequence.| acatctctgcagtcg... >gb|AF059851|ATCC6841|Mycobacterium fortuitum strain ATCC6841 16S ribosomal RNA (rrs)gene, partial sequence.| agtcgaacggaaagg... >gb|X52933|ATCC 6841|Mycobacterium fortuitum 16S rRNA gene.| ggcggcgtgcttaac...
23S rRNA Gene Sequence: >gb|U24519|ATCC 6841|Mycobacterium fortuitum 23S rRNA, partial sequence.| tgcgcttacaatccg...
Miscellaneous Sequence Data: >gb|AJ564405|ATCC 6841|Mycobacterium fortuitum partial GyrB gene for DNA gyrase subunit B,strain ATCC 6841.| gactcggcaggcggc...
Bibliography: CRUZ J C 1938 ACTA MED RIO DE JAN EIRO 1 297; GORDON R E & SMITH M M 1955
Extended Bibliography: showhide Show bibliography
Ref #: 95518
Author(s): Gingeras,T.R.;Ghandour,G.;Wang,E.;Berno,A.;Small,P.M.;Drobniewski,F.;Alland,D.;Desmond,E.;Holodniy,M.;Drenkow,J.
Journal: Genome Res
Title: Simultaneous genotyping and species identification using hybridization pattern recognition analysis of generic Mycobacterium DNA arrays
Volume: 8
Page(s): 435-48
Year: 1998
Keyword(s): GENBANK/AF059766 GENBANK/AF059767 GENBANK/AF059768 GENBANK/AF059769 GENBANK/AF059770 GENBANK/AF059771 GENBANK/AF059772 GENBANK/AF059773 GENBANK/AF059774 GENBANK/AF059775 GENBANK/AF059776 GENBANK/AF059777 GENBANK/AF059778 GENBANK/AF059779 GENBANK/AF059780 GENBANK/AF059781 GENBANK/AF059782 GENBANK/AF059783 GENBANK/AF059784 GENBANK/AF059785 GENBANK/AF059786 GENBANK/AF059787 GENBANK/AF059788 GENBANK/AF059789 GENBANK/AF059790 GENBANK/AF059791 GENBANK/AF059792 GENBANK/AF059793 GENBANK/AF059794 GENBANK/AF059795 Alleles DNA, Bacterial/*analysis DNA-Directed RNA Polymerases/genetics Drug Resistance, Microbial/genetics Gene Frequency Genes, Bacterial Genotype Molecular Sequence Data Mutagenesis Mycobacterium/drug effects/*genetics/*isolation & purification Mycobacterium tuberculosis/drug effects/genetics Nucleic Acid Hybridization/methods Oligonucleotides/analysis Polymorphism, Genetic RNA, Ribosomal, 16S/genetics Rifampin/pharmacology Sequence Analysis, DNA Species Specificity
Remarks: High-density oligonucleotide arrays can be used to rapidly examine large amounts of DNA sequence in a high throughput manner. An array designed to determine the specific nucleotide sequence of 705 bp of the rpoB gene of Mycobacterium tuberculosis accurately detected rifampin resistance associated with mutations of 44 clinical isolates of M. tuberculosis. The nucleotide sequence diversity in 121 Mycobacterial isolates (comprised of 10 species) was examined by both conventional dideoxynucleotide sequencing of the rpoB and 16S genes and by analysis of the rpoB oligonucleotide array hybridization patterns. Species identification for each of the isolates was similar irrespective of whether 16S sequence, rpoB sequence, or the pattern of rpoB hybridization was used. However, for several species, the number of alleles in the 16S and rpoB gene sequences provided discordant estimates of the genetic diversity within a species. In addition to confirming the array's intended utility for sequencing the region of M. tuberculosis that confers rifampin resistance, this work demonstrates that this array can identify the species of nontuberculous Mycobacteria. This demonstrates the general point that DNA microarrays that sequence important genomic regions (such as drug resistance or pathogenicity islands) can simultaneously identify species and provide some insight into the organism's population structure.
URL: 9582189
Ref #: 95444
Author(s): Talaat,A.M.;Reimschuessel,R.;Trucksis,M.
Journal: Vet Microbiol
Title: Identification of mycobacteria infecting fish to the species level using polymerase chain reaction and restriction enzyme analysis
Volume: 58
Page(s): 229-37
Year: 1998
Keyword(s): GENBANK/U92088 GENBANK/U92089 GENBANK/U92090 Animals Base Sequence Conserved Sequence DNA Primers Deoxyribonuclease BamHI Deoxyribonucleases, Type II Site-Specific *Fish Diseases Fishes Humans Mycobacterium/*classification/genetics/*isolation & purification Mycobacterium Infections/diagnosis/*veterinary Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics Restriction Mapping/*methods
Remarks: An assay is described utilizing PCR technology for a rapid diagnostic test to identify fish infection with Mycobacterium marinum, M. fortuitum and M. chelonae. A 924 bp DNA fragment from a highly conserved area of the mycobacterial 16S rRNA gene was amplified using mycobacteria genus-specific primers and digested with restriction enzymes (BanI and ApaI). This examination yielded unique restriction patterns for each mycobacterial specie enabling identification of mycobacteria infecting fish to the species level. The protocol can be applied to purified DNA, a simple colony preparation or infected fish tissue. This protocol can be completed in 1-2 days.
URL: 9453133
Ref #: 60196
Author(s): Kim,B.J.;Lee,S.H.;Lyu,M.A.;Kim,S.J.;Bai,G.H.;Chae,G.T.;Kim,E.C.;Cha,C.Y.;Kook,Y.H.
Journal: J Clin Microbiol
Title: Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB)
Volume: 37
Page(s): 1714-20
Year: 1999
Keyword(s): GENBANK/AF057449 GENBANK/AF057450 GENBANK/AF057451 GENBANK/AF057452 GENBANK/AF057453 GENBANK/AF057454 GENBANK/AF057455 GENBANK/AF057456 GENBANK/AF057457 GENBANK/AF057458 GENBANK/AF057459 GENBANK/AF057460 GENBANK/AF057461 GENBANK/AF057462 GENBANK/AF057463 GENBANK/AF057464 GENBANK/AF057465 GENBANK/AF057466 GENBANK/AF057467 GENBANK/AF057468 GENBANK/AF057469 GENBANK/AF057470 GENBANK/AF057471 GENBANK/AF057472 GENBANK/AF057473 GENBANK/AF057474 GENBANK/AF057475 GENBANK/AF057476 GENBANK/AF057477 GENBANK/AF057478 etc. Amino Acid Sequence DNA-Directed RNA Polymerases/chemistry/*genetics Humans Molecular Sequence Data Mycobacterium/*classification/enzymology/genetics Mycobacterium Infections/microbiology Phylogeny Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid
Remarks: For the differentiation and identification of mycobacterial species, the rpoB gene, encoding the beta subunit of RNA polymerase, was investigated. rpoB DNAs (342 bp) were amplified from 44 reference strains of mycobacteria and clinical isolates (107 strains) by PCR. The nucleotide sequences were directly determined (306 bp) and aligned by using the multiple alignment algorithm in the MegAlign package (DNASTAR) and the MEGA program. A phylogenetic tree was constructed by the neighbor-joining method. Comparative sequence analysis of rpoB DNAs provided the basis for species differentiation within the genus Mycobacterium. Slowly and rapidly growing groups of mycobacteria were clearly separated, and each mycobacterial species was differentiated as a distinct entity in the phylogenetic tree. Pathogenic Mycobacterium kansasii was easily differentiated from nonpathogenic M. gastri; this differentiation cannot be achieved by using 16S rRNA gene (rDNA) sequences. By being grouped into species-specific clusters with low-level sequence divergence among strains of the same species, all of the clinical isolates could be easily identified. These results suggest that comparative sequence analysis of amplified rpoB DNAs can be used efficiently to identify clinical isolates of mycobacteria in parallel with traditional culture methods and as a supplement to 16S rDNA gene analysis. Furthermore, in the case of M. tuberculosis, rifampin resistance can be simultaneously determined.
URL: 10325313
Ref #: 60037
Author(s): Hamid,M.E.;Roth,A.;Landt,O.;Kroppenstedt,R.M.;Goodfellow,M.;Mauch,H.
Journal: J Clin Microbiol
Title: Differentiation between Mycobacterium farcinogenes and Mycobacterium senegalense strains based on 16S-23S ribosomal DNA internal transcribed spacer sequences
Volume: 40
Page(s): 707-11
Year: 2002
Keyword(s): GENBANK/AJ291580 GENBANK/AJ291581 GENBANK/AJ291582 GENBANK/AJ291583 GENBANK/AJ291584 GENBANK/AJ291585 GENBANK/AJ291586 GENBANK/AJ291587 GENBANK/AJ291588 GENBANK/AJ291589 GENBANK/AJ291590 GENBANK/AJ291591 GENBANK/AJ291592 GENBANK/AJ291593 GENBANK/AJ291594 GENBANK/AJ291595 GENBANK/AJ291596 GENBANK/AJ291597 GENBANK/AJ291598 GENBANK/AJ291599 GENBANK/AJ291600 GENBANK/Y10384 GENBANK/Y10385 GENBANK/Y11581 GENBANK/Y11582 Animals Base Sequence Cattle Cattle Diseases/*microbiology DNA, Ribosomal Spacer/*genetics Molecular Sequence Data Mycobacterium/*classification/genetics Mycobacterium Infections/microbiology/*veterinary Phylogeny Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA
Remarks: 16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA sequence analyses were performed on Mycobacterium farcinogenes and M. senegalense strains and 26 strains of other rapidly growing mycobacteria to investigate the phylogenetic structure of bovine farcy mycobacteria within the M. fortuitum complex. M. farcinogenes and M. senegalense were indistinguishable in their 5"-end 16S rDNA but showed both considerable interspecies spacer sequence divergence and a high level of intraspecies sequence stability. A rapid detection assay using PCR and hybridization with species-specific probes was developed. The assay was specific among 46 species other than M. farcinogenes and M. senegalense and correctly identified all M. farcinogenes and M. senegalense strains. PCR- and 16S-23S rDNA sequence-based detection will be a valuable approach for diagnosis of the causal agents of African bovine farcy in cattle.
URL: 11826003
Ref #: 75210
Author(s): Stone,B.B.;Nietupski,R.M.;Breton,G.L.;Weisburg,W.G.
Journal: Int J Syst Bacteriol
Title: Comparison of Mycobacterium 23S rRNA sequences by high-temperature reverse transcription and PCR
Volume: 45
Page(s): 811-9
Year: 1995
Keyword(s): GENBANK/U24502 GENBANK/U24503 GENBANK/U24504 GENBANK/U24505 GENBANK/U24506 GENBANK/U24507 GENBANK/U24508 GENBANK/U24509 GENBANK/U24510 GENBANK/U24511 GENBANK/U24512 GENBANK/U24513 GENBANK/U24514 GENBANK/U24515 GENBANK/U24516 GENBANK/U24517 GENBANK/U24518 GENBANK/U24519 GENBANK/U24520 GENBANK/U24521 GENBANK/U24522 GENBANK/U24523 GENBANK/U24524 GENBANK/U24525 GENBANK/U24526 GENBANK/U24527 GENBANK/U24528 GENBANK/U24529 GENBANK/U24530 GENBANK/U24531 Base Sequence Molecular Sequence Data Mycobacterium/*genetics Phylogeny *Polymerase Chain Reaction RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/*chemistry Temperature Transcription, Genetic
Remarks: We describe a modified rRNA sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus Mycobacterium. We combined the sensitivity of the reverse transcriptase PCR for amplifying nanogram amounts of template rRNA material with the elevated extension temperatures used for the thermostable DNA polymerase from Thermus thermophilus. A 70 degrees C reverse transcription extension step permitted improved read-through of highly structured rRNA templates from members of the genus Mycobacterium, which have G+C contents of 66 to 71 mol%. The nucleic acid sequences of the amplified material were then determined by performing thermal cycle sequencing with alpha-33P-labeled primers, again with extension at 70 degrees C. Nonspecifically terminated bands were chased by using terminal deoxynucleotidyl transferase. Our method had a template requirement of nanogram amounts or less of purified RNA or 2,000 CFU of intact cells and had sufficient sensitivity so that lyophils obtained from the American Type Culture Collection could be used as source material. Sequences from a 250-nucleotide stretch of the 23S rRNA were aligned, and phylogenetic trees were evaluated by using the De Soete distance treeing algorithm and Rhodococcus bronchialis as the outgroup. Our 23S rRNA trees were compared with previously published 16S rRNA trees, including the comprehensive trees developed by the University of Illinois Ribosomal Database Project, and included 15 species not evaluated previously. Most of the groups were in general agreement and were consistent with relationships determined on the basis of biochemical characteristics, but some new relationships were also observed.
URL: 7547304
Ref #: 61936
Author(s): Lefmann,M.;Honisch,C.;Bocker,S.;Storm,N.;von Wintzingerode,F.;Schlotelburg,C.;Moter,A.;van den Boom,D.;Gobel,U.B.
Journal: J Clin Microbiol
Title: Novel mass spectrometry-based tool for genotypic identification of mycobacteria
Volume: 42
Page(s): 339-46
Year: 2004
Keyword(s): Genotype Mycobacterium/classification/*genetics Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
Remarks: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) after base-specific cleavage of PCR amplified and in vitro-transcribed 16S rRNA gene (rDNA) was used for the identification of mycobacteria. Full-length 16S rDNA reference sequences of 12 type strains of Mycobacterium spp. frequently isolated from clinical specimens were determined by PCR, cloning, and sequencing. For MALDI-TOF MS-based comparative sequence analysis, mycobacterial 16S rDNA signature sequences ( approximately 500 bp) of the 12 type strains and 24 clinical isolates were PCR amplified using RNA promoter-tagged forward primers. T7 RNA polymerase-mediated transcription of forward strands in the presence of 5-methyl ribo-CTP maximized mass differences of fragments generated by base-specific cleavage. In vitro transcripts were subsequently treated with RNase T1, resulting in G-specific cleavage. Sample analysis by MALDI-TOF MS showed a specific mass signal pattern for each of the 12 type strains, allowing unambiguous identification. All 24 clinical isolates were identified unequivocally by comparing their detected mass signal pattern to the reference sequence-derived in silico pattern of the type strains and to the in silico mass patterns of published 16S rDNA sequences. A 16S rDNA microheterogeneity of the Mycobacterium xenopi type strain (DSM 43995) was detected by MALDI-TOF MS and later confirmed by Sanger dideoxy sequencing. In conclusion, analysis of 16S rDNA amplicons by MS after base-specific cleavage of RNA transcripts allowed fast and reliable identification of the Mycobacterium tuberculosis complex and ubiquitous mycobacteria (mycobacteria other than tuberculosis). The technology delivers an open platform for high-throughput microbial identification on the basis of any specific genotypic marker region.
URL: 14715774
Ref #: 95467
Author(s): Menendez,M.C.;Garcia,M.J.;Navarro,M.C.;Gonzalez-y-Merchand,J.A.;Rivera-Gutierrez,S.;Garcia-Sanchez,L.;Cox,R.A.
Journal: J Bacteriol
Title: Characterization of an rRNA operon (rrnB) of Mycobacterium fortuitum and other mycobacterial species: implications for the classification of mycobacteria
Volume: 184
Page(s): 1078-88
Year: 2002
Keyword(s): Base Sequence DNA, Bacterial DNA, Intergenic *Genes, rRNA Molecular Sequence Data Mycobacterium/genetics Mycobacterium fortuitum/classification/*genetics Nucleic Acid Conformation *RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry Sequence Homology, Nucleic Acid Transcription Initiation Site *rRNA Operon
Remarks: Mycobacteria are thought to have either one or two rRNA operons per genome. All mycobacteria investigated to date have an operon, designated rrnA, located downstream from the murA gene. We report that Mycobacteriun fortuitum has a second rrn operon, designated rrnB, which is located downstream from the tyrS gene; tyrS is very close to the 3' end of a gene (3-mag) coding for 3-methylpurine-DNA-glycosylase. The second rrn operon of Mycobacterium smegmatis was shown to have a similar organization, namely, 5' 3-mag-tyrS-rrnB 3'. The rrnB operon of M. fortuitum was found to have a single dedicated promoter. During exponential growth in a rich medium, the rrnB and rrnA operons were the major and minor contributors, respectively, to pre-rRNA synthesis. Genomic DNA was isolated from eight other fast-growing mycobacterial species. Samples were investigated by Southern blot analysis using probes for murA, tyrS, and 16S rRNA sequences. The results revealed that both rrnA and rrnB operons were present in each species. The results form the basis for a proposed new scheme for the classification of mycobacteria. The approach, which is phylogenetic in concept, is based on particular properties of the rrn operons of a cell, namely, the number per genome and a feature of 16S rRNA gene sequences.
URL: 11807068
Ref #: 95465
Author(s): Dauendorffer,J.N.;Guillemin,I.;Aubry,A.;Truffot-Pernot,C.;Sougakoff,W.;Jarlier,V.;Cambau,E.
Journal: J Clin Microbiol
Title: Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes
Volume: 41
Page(s): 1311-5
Year: 2003
Keyword(s): Anti-Infective Agents/*pharmacology Base Sequence DNA Gyrase/*genetics DNA, Bacterial/analysis Drug Resistance, Bacterial/*genetics Fluoroquinolones Humans Microbial Sensitivity Tests Molecular Sequence Data Mycobacterium/drug effects/enzymology/*genetics Polymerase Chain Reaction Protein Structure, Tertiary/genetics Sequence Homology, Nucleic Acid
Remarks: The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance in mycobacteria. We looked to see if the assignment of mycobacterial species could be obtained simultaneously by analysis of the corresponding nucleotide sequences. PCR sequencing of gyrA and gyrB QRDRs was performed for 133 reference and clinical strains of 21 mycobacterial species commonly isolated in clinical laboratories. Nucleotide sequences of gyrA and gyrB QRDRs were species specific, regardless of fluoroquinolone susceptibility.
URL: 12624075
Ref #: 13165
Author(s): Talaat,A.M.;Reimschuessel,R.;Trucksis,M.
Journal: Vet Microbiol
Title: Identification of mycobacteria infecting fish to the species level using polymerase chain reaction and restriction enzyme analysis
Volume: 58
Page(s): 229-37
Year: 1998
Keyword(s): GENBANK/U92088 GENBANK/U92089 GENBANK/U92090 Animal Base Sequence Conserved Sequence DNA Primers Deoxyribonuclease BamHI Deoxyribonucleases, Type II Site-Specific *Fish Diseases Fishes Human Mycobacterium/*classification/genetics/*isolation & purification Mycobacterium Infections/diagnosis/*veterinary Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics Restriction Mapping/*methods Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S.
Remarks: An assay is described utilizing PCR technology for a rapid diagnostic test to identify fish infection with Mycobacterium marinum, M. fortuitum and M. chelonae. A 924 bp DNA fragment from a highly conserved area of the mycobacterial 16S rRNA gene was amplified using mycobacteria genus-specific primers and digested with restriction enzymes (BanI and ApaI). This examination yielded unique restriction patterns for each mycobacterial specie enabling identification of mycobacteria infecting fish to the species level. The protocol can be applied to purified DNA, a simple colony preparation or infected fish tissue. This protocol can be completed in 1-2 days.
URL: 98115223
Ref #: 13699
Author(s): Menendez,M.C.;Garcia,M.J.;Navarro,M.C.;Gonzalez-y-Merchand,J.A.;Rivera-Gutierrez,S.;Garcia-Sanchez,L.;Cox,R.A.
Journal: J Bacteriol
Title: Characterization of an rRNA operon (rrnB) of Mycobacterium fortuitum and other mycobacterial species: implications for the classification of mycobacteria
Volume: 184
Page(s): 1078-88
Year: 2002
Keyword(s): Base Sequence DNA, Bacterial DNA, Intergenic *Genes, rRNA Molecular Sequence Data Mycobacterium/genetics Mycobacterium fortuitum/classification/*genetics Nucleic Acid Conformation *RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Transcription Initiation Site *rRNA Operon
Remarks: Mycobacteria are thought to have either one or two rRNA operons per genome. All mycobacteria investigated to date have an operon, designated rrnA, located downstream from the murA gene. We report that Mycobacteriun fortuitum has a second rrn operon, designated rrnB, which is located downstream from the tyrS gene; tyrS is very close to the 3' end of a gene (3-mag) coding for 3-methylpurine-DNA-glycosylase. The second rrn operon of Mycobacterium smegmatis was shown to have a similar organization, namely, 5' 3-mag-tyrS-rrnB 3'. The rrnB operon of M. fortuitum was found to have a single dedicated promoter. During exponential growth in a rich medium, the rrnB and rrnA operons were the major and minor contributors, respectively, to pre-rRNA synthesis. Genomic DNA was isolated from eight other fast-growing mycobacterial species. Samples were investigated by Southern blot analysis using probes for murA, tyrS, and 16S rRNA sequences. The results revealed that both rrnA and rrnB operons were present in each species. The results form the basis for a proposed new scheme for the classification of mycobacteria. The approach, which is phylogenetic in concept, is based on particular properties of the rrn operons of a cell, namely, the number per genome and a feature of 16S rRNA gene sequences.
URL: 21666141
Ref #: 13682
Author(s): Hamid,M.E.;Roth,A.;Landt,O.;Kroppenstedt,R.M.;Goodfellow,M.;Mauch,H.
Journal: J Clin Microbiol
Title: Differentiation between Mycobacterium farcinogenes and Mycobacterium
Volume: 40
Page(s): 707-711
Year: 2002
Keyword(s): 0 (DNA, Ribosomal Spacer) 0 (RNA, Ribosomal, 16S) 0 (RNA, Ribosomal, 23S) Animal Base Sequence Cattle Cattle Diseases/*microbiology DNA, Ribosomal Spacer/*genetics Molecular Sequence Data Mycobacterium/*classification/genetics Mycobacterium Infections/microbiology/*veterinary Phylogeny Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Support, Non-U.S. Gov't
Remarks: 16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA
URL: 21683667
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 6442
Journal: Nucl. Acids Res.
Volume: 20
Year: 1992
Data: (ATCC 6841, TMC 1529) Type strain / R. E. Gordon, New Jersey in 1965 / J. C. Cruz in 1937 / Cold abscess / Cruz, J. C. (1938) Acta Med., Rio de Janeiro 1, 297 / Gordon, R. E. & Smith, M. M. (1955) J. Bact. 69, 502 / Gordon, R. E. & Mihm, J. M. (1959) J. gen. Microbiol. 21, 736 / Bojalil, L. F. et al. (1962) J. gen. Microbiol. 28, 333
Accession Date: 01/01/1965
History: ISOLATED BY CRUZ J C 1937-GORDON R E NEW JERSEY
Authority: da Costa Cruz 1938
Depositor: GORDON R E
Taxonomy: TaxLink: S1951 (Mycobacterium fortuitum subsp. fortuitum da Costa Cruz 1938) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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