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Bacteria Collection: Gordonia bronchialis

NCTC Number: NCTC 10667
Current Name: Gordonia bronchialis
Original Strain Reference: 410
Other Collection No: ATCC 25592; CATCC 25592; DSM 43247; JCM 3198; KCC A-0198
Previous Catalogue Name: Rhodococcus bronchialis
Type Strain: Yes
Family: Nocardiaceae
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1275665
16S rRNA Gene Sequence: >gb|X75903|NCTC 10667|G.bronchialis 16S rRNA gene.| gaacgggtgagtaac... >gb|AY262331|ATCC 25592|Gordonia bronchialis clone C2 16S ribosomal RNA gene, partialsequence.| tggagagtttgatcc...
23S rRNA Gene Sequence: >gb|U24559|ATCC 25592|Rhodococcus bronchialis 23S rRNA, partial sequence.| tgtacgtacaagcag...
Miscellaneous Sequence Data: >gb|AB014267|ATCC 25592|Gordonia bronchialis gyrB gene for DNA gyrase B subunit, partialcds, strain ATCC 25592.| tccgacgcctacgcc...
Extended Bibliography: showhide Show bibliography
Ref #: 75210
Author(s): Stone,B.B.;Nietupski,R.M.;Breton,G.L.;Weisburg,W.G.
Journal: Int J Syst Bacteriol
Title: Comparison of Mycobacterium 23S rRNA sequences by high-temperature reverse transcription and PCR
Volume: 45
Page(s): 811-9
Year: 1995
Keyword(s): GENBANK/U24502 GENBANK/U24503 GENBANK/U24504 GENBANK/U24505 GENBANK/U24506 GENBANK/U24507 GENBANK/U24508 GENBANK/U24509 GENBANK/U24510 GENBANK/U24511 GENBANK/U24512 GENBANK/U24513 GENBANK/U24514 GENBANK/U24515 GENBANK/U24516 GENBANK/U24517 GENBANK/U24518 GENBANK/U24519 GENBANK/U24520 GENBANK/U24521 GENBANK/U24522 GENBANK/U24523 GENBANK/U24524 GENBANK/U24525 GENBANK/U24526 GENBANK/U24527 GENBANK/U24528 GENBANK/U24529 GENBANK/U24530 GENBANK/U24531 Base Sequence Molecular Sequence Data Mycobacterium/*genetics Phylogeny *Polymerase Chain Reaction RNA, Bacterial/*chemistry RNA, Ribosomal, 16S/chemistry RNA, Ribosomal, 23S/*chemistry Temperature Transcription, Genetic
Remarks: We describe a modified rRNA sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus Mycobacterium. We combined the sensitivity of the reverse transcriptase PCR for amplifying nanogram amounts of template rRNA material with the elevated extension temperatures used for the thermostable DNA polymerase from Thermus thermophilus. A 70 degrees C reverse transcription extension step permitted improved read-through of highly structured rRNA templates from members of the genus Mycobacterium, which have G+C contents of 66 to 71 mol%. The nucleic acid sequences of the amplified material were then determined by performing thermal cycle sequencing with alpha-33P-labeled primers, again with extension at 70 degrees C. Nonspecifically terminated bands were chased by using terminal deoxynucleotidyl transferase. Our method had a template requirement of nanogram amounts or less of purified RNA or 2,000 CFU of intact cells and had sufficient sensitivity so that lyophils obtained from the American Type Culture Collection could be used as source material. Sequences from a 250-nucleotide stretch of the 23S rRNA were aligned, and phylogenetic trees were evaluated by using the De Soete distance treeing algorithm and Rhodococcus bronchialis as the outgroup. Our 23S rRNA trees were compared with previously published 16S rRNA trees, including the comprehensive trees developed by the University of Illinois Ribosomal Database Project, and included 15 species not evaluated previously. Most of the groups were in general agreement and were consistent with relationships determined on the basis of biochemical characteristics, but some new relationships were also observed.
URL: 7547304
Ref #: 65544
Author(s): Goodfellow,M.;Chun,J.;Stubbs,S.;Tobili,A.S.
Journal: Lett Appl Microbiol
Title: Transfer of Nocardia amarae Lechevalier and Lechevalier 1974 to the genus Gordona as Gordona amarae comb. nov
Volume: 19
Page(s): 401-5
Year: 1995
Keyword(s): GENBANK/X75902 Actinomycetales/*classification/genetics/metabolism Genes, Bacterial/genetics Lipids/analysis Molecular Sequence Data Nocardia/*classification/genetics/metabolism Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid
Remarks: The taxonomic status of Nocardia amarae strains was examined using chemical, microbiological and nucleic acid sequencing methods. It was evident from the results of this and previous studies that Nocardia amarae has properties that are at variance with its classification in the genus Nocardia but consistent with its transfer to the genus Gordona. It is proposed that Nocardia amarae Lechevalier and Lechevalier 1974 be transferred to the genus Gordona as Gordona amarae comb. nov.
URL: 7765703
Ref #: 65647
Author(s): Patel,J.B.;Wallace RJ,J.r.;Brown-Elliott,B.A.;Taylor,T.;Imperatrice,C.;Leonard,D.G.;Wilson,R.W.;Mann,L.;Jost,K.C.;Nachamkin,I.
Journal: J Clin Microbiol
Title: Sequence-based identification of aerobic actinomycetes
Volume: 42
Page(s): 2530-40
Year: 2004
Keyword(s): Actinobacteria/classification/genetics/*isolation & purification Aerobiosis DNA, Ribosomal/chemistry Nocardia/genetics/isolation & purification Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks: We investigated the utility of 500-bp 16S rRNA gene sequencing for identifying clinically significant species of aerobic actinomycetes. A total of 28 reference strains and 71 clinical isolates that included members of the genera Streptomyces, Gordonia, and Tsukamurella and 10 taxa of Nocardia were studied. Methods of nonsequencing analyses included growth and biochemical analysis, PCR-restriction enzyme analysis of the 439-bp Telenti fragment of the 65 hsp gene, susceptibility testing, and, for selected isolates, high-performance liquid chromatography. Many of the isolates were included in prior taxonomic studies. Sequencing of Nocardia species revealed that members of the group were generally most closely related to the American Type Culture Collection (ATCC) type strains. However, the sequences of Nocardia transvalensis, N. otitidiscaviarum, and N. nova isolates were highly variable; and it is likely that each of these species contains multiple species. We propose that these three species be designated complexes until they are more taxonomically defined. The sequences of several taxa did not match any recognized species. Among other aerobic actinomycetes, each group most closely resembled the associated reference strain, but with some divergence. The study demonstrates the ability of partial 16S rRNA gene sequencing to identify members of the aerobic actinomycetes, but the study also shows that a high degree of sequence divergence exists within many species and that many taxa within the Nocardia spp. are unnamed at present. A major unresolved issue is the type strain of N. asteroides, as the present one (ATCC 19247), chosen before the availability of molecular analysis, does not represent any of the common taxa associated with clinical nocardiosis.
URL: 15184431
Ref #: 95449
Author(s): Shen,F.T.;Lu,H.L.;Lin,J.L.;Huang,W.S.;Arun,A.B.;Young,C.C.
Journal: Res Microbiol
Title: Phylogenetic analysis of members of the metabolically diverse genus Gordonia based on proteins encoding the gyrB gene
Volume: 157
Page(s): 367-75
Year: 2006
Keyword(s): Amino Acid Sequence DNA Gyrase/genetics/*metabolism DNA Primers Gordonia Bacterium/*classification/enzymology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Homology, Amino Acid
Remarks: Members of the metabolically diverse genus Gordonia were isolated from various biotopes including pristine and polluted sites around Taiwan. Identification, comparison and diversity assessment based on the gyrB gene were carried out using a newly developed primer pair for gyrB. The 16S rRNA gene was also sequenced for comparison. A 1.2-kb fragment of the gyrB gene of 17 Gordonia strains including type strains was determined by direct sequencing of PCR amplified fragments. A total of 25 strains (8 of which were retrieved from a public database) of the genus Gordonia form a distinct phyletic line in the GyrB-based tree and are separated from other closely related species of genera of the suborder Corynebacterineae. Sequence similarity of the gyrB sequence from twelve Gordonia type strains ranged from 79.3 to 97.2%, corresponding to between 270 and 41 nucleotide differences, while there was only a 0.3-3.8% difference in 16S rRNA gene sequence similarity at the interspecies level. Phylogenetic analysis based on the GyrB sequence deduced from the gyrB gene is consistent with that of DNA-DNA hybridization results and provides a better discrimination within the species of Gordonia compared to the 16S rRNA gene. The present study demonstrates that gyrB gene analysis will aid in describing novel species belonging to the genus Gordonia.
URL: 16310344
Ref #: 12782
Author(s): Goodfellow,M.;Chun,J.;Stubbs,S.;Tobili,A.S.
Journal: Lett Appl Microbiol
Title: Transfer of Nocardia amarae Lechevalier and Lechevalier 1974 to the genus Gordona as Gordona amarae comb. nov
Volume: 19
Page(s): 401-5
Year: 1995
Keyword(s): GENBANK/X75902 Actinomycetales/*classification/genetics/metabolism Genes, Bacterial/genetics Lipids/analysis Molecular Sequence Data Nocardia/*classification/genetics/metabolism Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't
Remarks: The taxonomic status of Nocardia amarae strains was examined using chemical, microbiological and nucleic acid sequencing methods. It was evident from the results of this and previous studies that Nocardia amarae has properties that are at variance with its classification in the genus Nocardia but consistent with its transfer to the genus Gordona. It is proposed that Nocardia amarae Lechevalier and Lechevalier 1974 be transferred to the genus Gordona as Gordona amarae comb. nov.
URL: 95118578
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 2184
Author(s): Goodfellow,M.;Alderson,G.
Journal: J. Gen. Microbiol.
Title: The actinomycete-genus Rhodococcus: a home for the "rhodochrous" complex.
Volume: 100
Page(s): 99-122
Year: 1977
Data: (ATCC 25592) Type strain / M. Tsukamura, Obu, Chita, Aichi-ken, Japan in 1970 / H. Kondo in 1968 / Sputum, pulmonary lesion
Accession Date: 04/02/2003
Authority: (Tsukamura 1971) Stackebrandt et al. 1989
Taxonomy: TaxLink: S1373 (Gordonia bronchialis (tsukamura 1971) stackebrandt et al. 1989) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: ? (NCTC 10667) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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