Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Pseudomonas lemoignei

NCTC Number: NCTC 10937
Current Name: Pseudomonas lemoignei
Original Strain Reference: ATCC 17989
Other Collection No: ATCC 17989
Previous Catalogue Name: Pseudomonas lemoignei
Type Strain: Yes
Family: Pseudomonadaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 30°C, aerobic
Conditions for growth on liquid media: nutrint broth,30, aerobic
Isolated From: soil;poly-b-hydroxybutyrate enriched soil
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1018582
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|Z93439|ATCC 17979|DNA GROUP SIX|Acinetobacter sp. 16S rRNA gene (strain ATCC 17979).| cctggctcagattga...
Bibliography: DELAFIELD F P ET AL 1965 J BACT 90 1455
Extended Bibliography: showhide Show bibliography
Ref #: 15405
Author(s): Yamamoto,S.;Bouvet,P.J.;Harayama,S.
Journal: Int J Syst Bacteriol
Title: Phylogenetic structures of the genus Acinetobacter based on gyrB sequences: comparison with the grouping by DNA-DNA hybridization
Volume: 49 Pt 1
Page(s): 87-95
Year: 1999
Keyword(s): GENBANK/AB008684 GENBANK/AB008685 GENBANK/AB008686 GENBANK/AB008687 GENBANK/AB008688 GENBANK/AB008689 GENBANK/AB008690 GENBANK/AB008691 GENBANK/AB008692 GENBANK/AB008693 GENBANK/AB008694 GENBANK/AB008695 GENBANK/AB008696 GENBANK/AB008697 GENBANK/AB008698 GENBANK/AB008699 GENBANK/AB008700 GENBANK/AB008701 GENBANK/AB008702 GENBANK/AB008703 GENBANK/AB008704 GENBANK/AB008705 GENBANK/AB008706 GENBANK/AB008707 GENBANK/AB008708 GENBANK/AB008709 GENBANK/AB008710 GENBANK/AB008711 GENBANK/AB008712 GENBANK/AB008713 Acinetobacter/*classification/genetics Base Sequence DNA Gyrase DNA Topoisomerases, Type II/*genetics DNA, Bacterial/*analysis Molecular Sequence Data *Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S/chemistry
Remarks: The phylogenetic relationships of 49 Acinetobacter strains, 46 of which have previously been classified into 18 genomic species by DNA-DNA hybridization studies, were investigated using the nucleotide sequence of gyrB, the structural gene for the DNA gyrase B subunit. The phylogenetic tree showed linkages between genomic species 1 (Acinetobacter calcoaceticus), 2 (Acinetobacter baumannii), 3 and TU13; genomic species 6, BJ15, BJ16 and BJ17; genomic species 5, BJ13 (synonym of TU14) and BJ14; genomic species 7 (Acinetobacter johnsonii), 10 and 11; and genomic species 8 and 9. The phylogenetic grouping of Acinetobacter strains based on gyrB genes was almost congruent with that based on DNA-DNA hybridization studies. Consequently, gyrB sequence comparison can be used to resolve the taxonomic positions of bacterial strains at the level of genomic species. However, minor discrepancies existed in the grouping of strains of genomic species 8, 9 and BJ17. The phylogenetic tree for these strains was reconstructed from the sequence of rpoD, the structural gene for the RNA polymerase sigma 70 factor. The latter tree was 100% congruent with the grouping based on DNA-DNA hybridization. The reliability of DNA-DNA hybridization may be superior to that of sequence comparison of a single protein-encoding gene in resolving closely related organisms since the former method measures the homologies between the nucleotide sequences of total genomic DNAs. Three strains that have not been characterized previously by DNA-DNA hybridization seem to belong to two new genomic species, one including strain ATCC 33308 and the other including strains ATCC 31012 and MBIC 1332.
URL: 10028249
Ref #: 15314
Author(s): Ibrahim,A.;Gerner-Smidt,P.;Liesack,W.
Journal: Int J Syst Bacteriol
Title: Phylogenetic relationship of the twenty-one DNA groups of the genus Acinetobacter as revealed by 16S ribosomal DNA sequence analysis
Volume: 47
Page(s): 837-41
Year: 1997
Keyword(s): GENBANK/Z93434 GENBANK/Z93435 GENBANK/Z93436 GENBANK/Z93437 GENBANK/Z93438 GENBANK/Z93439 GENBANK/Z93440 GENBANK/Z93441 GENBANK/Z93442 GENBANK/Z93443 GENBANK/Z93444 GENBANK/Z93445 GENBANK/Z93446 GENBANK/Z93447 GENBANK/Z93448 GENBANK/Z93449 GENBANK/Z93450 GENBANK/Z93451 GENBANK/Z93452 GENBANK/Z93453 GENBANK/Z93454 Acinetobacter/*classification/*genetics DNA Primers DNA, Bacterial/analysis DNA, Ribosomal/*analysis Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA
Remarks: The inter- and intrageneric relationships of members of the genus Acinetobacter were investigated by performing a comparative sequence analysis of PCR-amplified 16S ribosomal DNAs (rDNAs) from 21 strains representing all of the DNA groups that have been described. Phylogenetic treeing confirmed that Acinetobacter spp. form a coherent cluster within the gamma subdivision of the class Proteobacteria that includes strains with overall levels of 16S rDNA sequence similarity of more than 94%. The analysis of intrageneric relationships suggested that the majority of the strains cluster in five clearly distinguishable clusters, and this conclusion was supported by the results obtained with the different methods used for phylogenetic analysis (i.e., the maximum-likelihood, parsimony, and distance matrix methods). The first cluster contains the representatives of DNA groups 2 (Acinetobacter baumannii) and TU13, whereas the second cluster comprises representatives of DNA groups 3, "Close To TU13," and "between 1 and 3." The representatives of closely related Acinetobacter DNA groups 8 (Acinetobacter twoffii) and 9 belong to the third cluster, which includes the representative of DNA group 6 as well. The fourth cluster is formed by DNA groups BJ15, BJ16, and BJ17, and the fifth cluster comprises DNA groups 1 (Acinetobacter calcoaceticus), BJ14, 10, and 11. Within the fifth cluster the 16S rDNA sequences of DNA group 10 and 11 strains are nearly identical. The representatives of DNA groups 4 (Acinetobacter haemolyticus), 5 (Acinetobacter junii), 7 (Acinetobacter johnsonii), 12 (Acinetobacter radioresistens), TU14, and TU15 form individual branches that are not significantly affiliated with any of the five clusters identified. Apart from the clustering of the most closely related DNA groups, the general topology of the distance dendrogram revealed some discrepancy with previous DNA-DNA hybridization data, which may point to the inadequacy of comparative 16S rDNA sequence analysis for reflecting true evolutionary relationships of closely related bacterial taxa. Important, however, was the presence of unique sequence motifs in each of the 21 different DNA groups studied, which may be useful for rapid differentiation of DNA groups of the genus Acinetobacter.
URL: 9226915
Ref #: 11979
Author(s): Ibrahim,A.;Gerner-Smidt,P.;Liesack,W.
Journal: Int J Syst Bacteriol
Title: Phylogenetic relationship of the twenty-one DNA groups of the genus Acinetobacter as revealed by 16S ribosomal DNA sequence analysis
Volume: 47
Page(s): 837-41
Year: 1997
Keyword(s): GENBANK/Z93434 GENBANK/Z93435 GENBANK/Z93436 GENBANK/Z93437 GENBANK/Z93438 GENBANK/Z93439 GENBANK/Z93440 GENBANK/Z93441 GENBANK/Z93442 GENBANK/Z93443 GENBANK/Z93444 GENBANK/Z93445 GENBANK/Z93446 GENBANK/Z93447 GENBANK/Z93448 GENBANK/Z93449 GENBANK/Z93450 GENBANK/Z93451 GENBANK/Z93452 GENBANK/Z93453 GENBANK/Z93454 Acinetobacter/*classification/*genetics DNA Primers DNA, Bacterial/analysis DNA, Ribosomal/*analysis Molecular Sequence Data Nucleic Acid Hybridization *Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA
Remarks: The inter- and intrageneric relationships of members of the genus Acinetobacter were investigated by performing a comparative sequence analysis of PCR-amplified 16S ribosomal DNAs (rDNAs) from 21 strains representing all of the DNA groups that have been described. Phylogenetic treeing confirmed that Acinetobacter spp. form a coherent cluster within the gamma subdivision of the class Proteobacteria that includes strains with overall levels of 16S rDNA sequence similarity of more than 94%. The analysis of intrageneric relationships suggested that the majority of the strains cluster in five clearly distinguishable clusters, and this conclusion was supported by the results obtained with the different methods used for phylogenetic analysis (i.e., the maximum-likelihood, parsimony, and distance matrix methods). The first cluster contains the representatives of DNA groups 2 (Acinetobacter baumannii) and TU13, whereas the second cluster comprises representatives of DNA groups 3, "Close To TU13," and "between 1 and 3." The representatives of closely related Acinetobacter DNA groups 8 (Acinetobacter twoffii) and 9 belong to the third cluster, which includes the representative of DNA group 6 as well. The fourth cluster is formed by DNA groups BJ15, BJ16, and BJ17, and the fifth cluster comprises DNA groups 1 (Acinetobacter calcoaceticus), BJ14, 10, and 11. Within the fifth cluster the 16S rDNA sequences of DNA group 10 and 11 strains are nearly identical. The representatives of DNA groups 4 (Acinetobacter haemolyticus), 5 (Acinetobacter junii), 7 (Acinetobacter johnsonii), 12 (Acinetobacter radioresistens), TU14, and TU15 form individual branches that are not significantly affiliated with any of the five clusters identified. Apart from the clustering of the most closely related DNA groups, the general topology of the distance dendrogram revealed some discrepancy with previous DNA-DNA hybridization data, which may point to the inadequacy of comparative 16S rDNA sequence analysis for reflecting true evolutionary relationships of closely related bacterial taxa. Important, however, was the presence of unique sequence motifs in each of the 21 different DNA groups studied, which may be useful for rapid differentiation of DNA groups of the genus Acinetobacter.
URL: 97370609
Data: Type strain / ATCC in 1973 / N. J. Palleroni / Poly--hydroxybutyrate enriched soil / Delafield, F. P. et al. (1965) J. Bact. 90, 1455
Accession Date: 01/01/1973
History: ISOLATED BY PALLERONI N J
Authority: Delafield et al. 1965 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S2453 (Pseudomonas lemoignei) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

Note: Links open in a new window

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.