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Bacteria Collection: Streptococcus constellatus

NCTC Number: NCTC 11325
Current Name: Streptococcus constellatus
Original Strain Reference: VPI 3810, Prevot 4055
Other Collection No: ATCC 27823; PREVOT 4055; V.P.I. 3810
Previous Catalogue Name: Streptococcus constellatus
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, anaerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, patient with purulent pleurisy
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1247829
16S rRNA Gene Sequence: >gb|AF104676|ATCC27823|Streptococcus constellatus strain ATCC27823 16S ribosomal RNA gene,partial sequence.| tttgatcmtggttca... >gb|Z69041|ATCC 27823|S.constellatus 16S ribosomal RNA.| atgcaagtagaacgc... >gb|AY347546|ATCC 27823|Streptococcus constellatus subsp. constellatus strain ATCC 2782316S-23S ribosomal RNA intergenic spacer, complete sequence.| aggaaaaatcggaag...
23S rRNA Gene Sequence: >gb|AY347546|ATCC 27823|Streptococcus constellatus subsp. constellatus strain ATCC 2782316S-23S ribosomal RNA intergenic spacer, complete sequence.| aggaaaaatcggaag...
Bibliography: HOLDEMAN L V & MOORE W E C 1974 INT.J.SYST.BACT. 24 268
Extended Bibliography: showhide Show bibliography
Ref #: 82754
Author(s): Jacobs,J.A.;Schot,C.S.;Schouls,L.M.
Journal: J Med Microbiol
Title: Haemolytic activity of the Streptococcus milleri group' and relationship between haemolysis restricted to human red blood cells and pathogenicity in S. intermedius
Volume: 49
Page(s): 55-62
Year: 2000
Keyword(s): Animals Cattle Female Genotype *Hemolysis Horses Humans Male Phenotype Phylogeny RNA, Ribosomal, 16S/chemistry/genetics Rabbits Sheep Species Specificity Streptococcus/classification/genetics/*pathogenicity Swine Variation (Genetics)
Remarks: A collection of 297 clinically documented 'Streptococcus milleri' strains, identified to the genotype level by 16S rRNA gene hydridisation, was screened for haemolysis of human and animal red blood cells. Forty-nine strains (65%) of the S. intermedius genotype displayed haemolysis restricted to human blood; they were named 'exclusive human haemolytic' (EHH) S. intermedius strains. The 26 remaining S. intermedius strains were named S. intermedius non-EHH strains. Quantitative studies on the haemolysis indicated that intermedilysin was the factor involved. The S. intermedius EHH strains represented the S. intermedius phenotype, whereas the S. intermedius non-EHH strains were phenotypically characteristic of S. constellatus. The complete 16S rRNA sequences of the S. intermedius EHH strains exhibited identity with S. intermedius strains ATCC 27335 (= NCDO 2227, NCTC 11324); the 16S rRNA sequences of the S. intermedius non-EHH strains were identical to S. constellatus strain ATCC 27823 (= NCDO 2226, NCTC 11325) except for positions 228 and 229 that carried an S. intermedius sequence signature. The 16S sequence similarities between the non-EHH strains and the S. constellatus and the S. intermedius type strains were 99.5% and 98.6%, respectively. Hybridisations of the complete 16S rRNA genes with oligonucleotide probes indicated a 16S rRNA homogeneity within the S. intermedius EHH and the non-EHH strains respectively. The S. intermedius EHH strains were isolated most frequently from infection- and abscess-related specimens. The present data emphasise the genetic variability within the S. constellatus species and redefine the S. intermedius species as a homogeneous group at the 16S rRNA level.
URL: 10628826
Ref #: 95486
Author(s): Jacobs,J.A.;Schot,C.S.;Bunschoten,A.E.;Schouls,L.M.
Journal: J Clin Microbiol
Title: Rapid species identification of "Streptococcus milleri" strains by line blot hybridization: identification of a distinct 16S rRNA population closely related to Streptococcus constellatus
Volume: 34
Page(s): 1717-21
Year: 1996
Keyword(s): GENBANK/Z69037 GENBANK/Z69038 GENBANK/Z69040 GENBANK/Z69041 Bacterial Typing Techniques Base Sequence DNA Probes/genetics Genes, Bacterial Humans Molecular Sequence Data Nucleic Acid Hybridization Phenotype Polymerase Chain Reaction RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Species Specificity Streptococcus/*classification/*genetics/isolation & purification
Remarks: A collection of 399 "Streptococcus milleri" strains were identified to the species level by the use of a line blot assay. Their PCR-amplified partial 16S rRNA gene sequences were hybridized with species-specific 5'-biotinylated oligonucleotide probes homologous to the bp 213 to 231 regions of the 16S rRNA gene sequences of the type strains Streptococcus anginosus ATCC 33397, Streptococcus constellatus ATCC 27823, and Streptococcus intermedius ATCC 27335. The hybridization results were compared with the reference phenotypic identification method data (R. A. Whiley, H. Fraser, J. M. Hardie, and D. Beighton, J. Clin. Microbiol. 28:1497-1501, 1990). Most strains (357 of 399 [89.5%]) reacted unambiguously with only one probe. However, 42 of the 399 strains (10.5%) reacted with both the S. constellatus- and S. intermedius-specific probes; 41 of them were phenotypically identified as S. constellatus. These dually reactive strains hybridized with a 5'-biotinylated probe based on the bp 213 to 231 region of the 16S rRNA gene sequence of one of two species. Analysis of the 5' ends of the 16S rRNA gene sequences (487 bp) demonstrated that the dually reactive strains represent a distinct rRNA population sharing 98.1% sequence similarity with S. constellatus. Phenotypic consistency between the dually reactive strains and the S. constellatus strains was not demonstrated. Line blot hybridization proved to be a simple and inexpensive method to screen large numbers of strains for genetic relatedness, and it allowed the detection of a distinct 16S rRNA type within the "S. milleri" group.
URL: 8784576
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 13410
Author(s): Jacobs,J.A.;Schot,C.S.;Bunschoten,A.E.;Schouls,L.M.
Journal: J Clin Microbiol
Title: Rapid species identification of "Streptococcus milleri" strains by line blot hybridization: identification of a distinct 16S rRNA population closely related to Streptococcus constellatus
Volume: 34
Page(s): 1717-21
Year: 1996
Keyword(s): GENBANK/Z69037 GENBANK/Z69038 GENBANK/Z69040 GENBANK/Z69041 Bacterial Typing Techniques Base Sequence Comparative Study DNA Probes/genetics Genes, Bacterial Human Molecular Sequence Data Nucleic Acid Hybridization Phenotype Polymerase Chain Reaction RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Species Specificity Streptococcus/*classification/*genetics/isolation & purification
Remarks: A collection of 399 "Streptococcus milleri" strains were identified to the species level by the use of a line blot assay. Their PCR-amplified partial 16S rRNA gene sequences were hybridized with species-specific 5'-biotinylated oligonucleotide probes homologous to the bp 213 to 231 regions of the 16S rRNA gene sequences of the type strains Streptococcus anginosus ATCC 33397, Streptococcus constellatus ATCC 27823, and Streptococcus intermedius ATCC 27335. The hybridization results were compared with the reference phenotypic identification method data (R. A. Whiley, H. Fraser, J. M. Hardie, and D. Beighton, J. Clin. Microbiol. 28:1497-1501, 1990). Most strains (357 of 399 [89.5%]) reacted unambiguously with only one probe. However, 42 of the 399 strains (10.5%) reacted with both the S. constellatus- and S. intermedius-specific probes; 41 of them were phenotypically identified as S. constellatus. These dually reactive strains hybridized with a 5'-biotinylated probe based on the bp 213 to 231 region of the 16S rRNA gene sequence of one of two species. Analysis of the 5' ends of the 16S rRNA gene sequences (487 bp) demonstrated that the dually reactive strains represent a distinct rRNA population sharing 98.1% sequence similarity with S. constellatus. Phenotypic consistency between the dually reactive strains and the S. constellatus strains was not demonstrated. Line blot hybridization proved to be a simple and inexpensive method to screen large numbers of strains for genetic relatedness, and it allowed the detection of a distinct 16S rRNA type within the "S. milleri" group.
URL: 96379013
Ref #: 13390
Author(s): Jacobs,J.A.;Schot,C.S.;Schouls,L.M.
Journal: J Med Microbiol
Title: Haemolytic activity of the Streptococcus milleri group' and relationship between haemolysis restricted to human red blood cells and pathogenicity in S. intermedius
Volume: 49
Page(s): 55-62
Year: 2000
Keyword(s): Animal Cattle Female Genotype *Hemolysis Horses Human Male Phenotype Phylogeny RNA, Ribosomal, 16S/chemistry/genetics Rabbits Sheep Species Specificity Streptococcus/classification/genetics/*pathogenicity Swine Variation (Genetics)
Remarks: A collection of 297 clinically documented 'Streptococcus milleri' strains, identified to the genotype level by 16S rRNA gene hydridisation, was screened for haemolysis of human and animal red blood cells. Forty-nine strains (65%) of the S. intermedius genotype displayed haemolysis restricted to human blood; they were named 'exclusive human haemolytic' (EHH) S. intermedius strains. The 26 remaining S. intermedius strains were named S. intermedius non-EHH strains. Quantitative studies on the haemolysis indicated that intermedilysin was the factor involved. The S. intermedius EHH strains represented the S. intermedius phenotype, whereas the S. intermedius non-EHH strains were phenotypically characteristic of S. constellatus. The complete 16S rRNA sequences of the S. intermedius EHH strains exhibited identity with S. intermedius strains ATCC 27335 (= NCDO 2227, NCTC 11324); the 16S rRNA sequences of the S. intermedius non-EHH strains were identical to S. constellatus strain ATCC 27823 (= NCDO 2226, NCTC 11325) except for positions 228 and 229 that carried an S. intermedius sequence signature. The 16S sequence similarities between the non-EHH strains and the S. constellatus and the S. intermedius type strains were 99.5% and 98.6%, respectively. Hybridisations of the complete 16S rRNA genes with oligonucleotide probes indicated a 16S rRNA homogeneity within the S. intermedius EHH and the non-EHH strains respectively. The S. intermedius EHH strains were isolated most frequently from infection- and abscess-related specimens. The present data emphasise the genetic variability within the S. constellatus species and redefine the S. intermedius species as a homogeneous group at the 16S rRNA level.
URL: 20092448
Data: (ATCC 27823, NCDO 2226) Type strain / ATCC in 1979 / L. V. Holdeman, V. P. I. / A. Prevot, Paris / Probably from case of purulent pleurisy / Holdeman, L. V. and Moore, W. E. C. (1974) Int. J. syst. Bact. 24 268 / Whiley, R. A. & Beighton, D. (1991) Int. J. syst. Bact. 41, 1
Accession Date: 01/01/1979
History: ISOLATED BY PREVOT A ,PARIS,HOLDEMAN L V ,V.P.I.;ATCC ,1979 PRE:FR
Authority: Prévot 1924 emend. Whiley and Beighton 1991
Depositor: GHERNA R L
Taxonomy: TaxLink: S2829 (Streptococcus constellatus subsp. constellatus (Prévot 1924) Holdeman and Moore 1974 emend. Whiley and Beighton 1991 emend. Whiley et al. 1999) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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