Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Eggerthella lenta

NCTC Number: NCTC 11813
Current Name: Eggerthella lenta
Original Strain Reference: VPI 0255
Other Collection No: ATCC 25559; DSM 2243; VPI 0255
Previous Catalogue Name: Eubacterium lentum
Type Strain: Yes
Family: Coriobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48 hours, 37°C, anaerobic
Conditions for growth on liquid media: nutrient broth,
Isolated From: human, rectal tumour
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1295517
16S rRNA Gene Sequence: >gb|AF292375|ATCC25559|Eggerthella lenta 16S ribosomal RNA gene, partial sequence.| agagtttgatcctgg...
Bibliography: PREVOT A H 1938 ANNLS INST PASTEUR PARIS 60 285; EGGERTH A H 1935 J BACT
Extended Bibliography: showhide Show bibliography
Ref #: 21346
Author(s): Dewhirst,F.E.;Paster,B.J.;Tzellas,N.;Coleman,B.;Downes,J.;Spratt,D.A.;Wade,W.G.
Journal: Int J Syst Evol Microbiol
Title: Characterization of novel human oral isolates and cloned 16S rDNA sequences that fall in the family Coriobacteriaceae: description of olsenella gen. nov., reclassification of Lactobacillus uli as Olsenella uli comb. nov. and description of Olsenella profusa sp. nov
Volume: 51
Page(s): 1797-804
Year: 2001
Keyword(s): GENBANK/AF287760 GENBANK/AF292371 GENBANK/AF292372 GENBANK/AF292373 GENBANK/AF292374 GENBANK/AF292375 Actinobacteria/*classification/*genetics Cloning, Molecular/methods DNA, Ribosomal/analysis Dental Plaque/*microbiology Genes, rRNA Gingiva/*microbiology Humans Lactobacillus/*classification/genetics Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks: The diversity of organisms present in the subgingival pockets of patients with periodontitis and acute necrotizing ulcerative gingivitis (ANUG) were examined previously. The 16S rRNA genes of subgingival plaque bacteria were amplified using PCR with a universal forward primer and a spirochaete-selective reverse primer. The amplified DNA was cloned into Escherichia coli. In one subject with ANUG, 70 clones were sequenced. Seventy-five per cent of the clones were spirochaetal, as expected. Twelve of the remaining clones fell into two clusters that represent novel phylotypes in the family Coriobacteriaceae. The first novel phylotype was most closely related to Atopobium rimae (98% similarity). The phylotype probably represents a novel Atopobium species, but will not be named until cultivable strains are obtained. The second novel phylotype was only 91% similar to described Atopobium species and 84% similar to Coriobacterium glomerans. The 16S rRNA sequences of the type strain of Lactobacillus uli and a strain representing the Moores' Eubacterium group D52 were determined as part of on ongoing sequence analysis of oral bacteria. The sequence for L. uli was more than 99.8% similar to sequences for the second clone phylotype. It therefore appears that the second clone phylotype and L. uli represent the same species. The sequence for the Eubacterium D52 strain was 95.6% similar to that of L. uli. The G+C content of the DNA of L. uli and Eubacterium D52 is 63-64 mol %. These organisms are thus distinct from the neighbouring genus Atopobium, which has a DNA G+C content of 35-46 mol%. A new genus, Olsenella gen. nov., is proposed for these two species on the basis of phenotypic characteristics and 16S rRNA sequence analysis to include Olsenella uli comb. nov. and Olsenella profusa sp. nov.
URL: 11594611
Ref #: 12165
Author(s): Dewhirst,F.E.;Paster,B.J.;Tzellas,N.;Coleman,B.;Downes,J.;Spratt,D.A.;Wade,W.G.
Journal: Int J Syst Evol Microbiol
Title: Characterization of novel human oral isolates and cloned 16S rDNA sequences that fall in the family Coriobacteriaceae: description of olsenella gen. nov., reclassification of Lactobacillus uli as Olsenella uli comb. nov. and description of Olsenella profusa sp. nov
Volume: 51
Page(s): 1797-804
Year: 2001
Keyword(s): GENBANK/AF287760 GENBANK/AF292371 GENBANK/AF292372 GENBANK/AF292373 GENBANK/AF292374 GENBANK/AF292375 Actinobacteria/*classification/*genetics Cloning, Molecular/methods DNA, Ribosomal/analysis Dental Plaque/*microbiology Genes, rRNA Gingiva/*microbiology Human Lactobacillus/*classification/genetics Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
Remarks: The diversity of organisms present in the subgingival pockets of patients with periodontitis and acute necrotizing ulcerative gingivitis (ANUG) were examined previously. The 16S rRNA genes of subgingival plaque bacteria were amplified using PCR with a universal forward primer and a spirochaete-selective reverse primer. The amplified DNA was cloned into Escherichia coli. In one subject with ANUG, 70 clones were sequenced. Seventy-five per cent of the clones were spirochaetal, as expected. Twelve of the remaining clones fell into two clusters that represent novel phylotypes in the family Coriobacteriaceae. The first novel phylotype was most closely related to Atopobium rimae (98% similarity). The phylotype probably represents a novel Atopobium species, but will not be named until cultivable strains are obtained. The second novel phylotype was only 91% similar to described Atopobium species and 84% similar to Coriobacterium glomerans. The 16S rRNA sequences of the type strain of Lactobacillus uli and a strain representing the Moores' Eubacterium group D52 were determined as part of on ongoing sequence analysis of oral bacteria. The sequence for L. uli was more than 99.8% similar to sequences for the second clone phylotype. It therefore appears that the second clone phylotype and L. uli represent the same species. The sequence for the Eubacterium D52 strain was 95.6% similar to that of L. uli. The G+C content of the DNA of L. uli and Eubacterium D52 is 63-64 mol %. These organisms are thus distinct from the neighbouring genus Atopobium, which has a DNA G+C content of 35-46 mol%. A new genus, Olsenella gen. nov., is proposed for these two species on the basis of phenotypic characteristics and 16S rRNA sequence analysis to include Olsenella uli comb. nov. and Olsenella profusa sp. nov.
URL: 21477803
Data: (ATCC 25559, DSM 2243) Type strain / ATCC / A. H. Eggerth / Rectal tumour / Prevot, A. H. (1938) Annls. Inst. Pasteur Paris 60, 285 / Eggerth, A. H. (1935) J. Bact. 30, 277
Accession Date: 01/01/1985
Authority: (Eggerth 1935) Wade et al. 1999
Depositor: EGGERTH A H
Taxonomy: TaxLink: S1217 (Eubacterium lentum) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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