Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Streptococcus mitis

NCTC Number: NCTC 12261
Current Name: Streptococcus mitis
Original Strain Reference: 12261 SK 142
Other Collection No: ATCC 49456; DSM 12643; JCM 12971; KCTC 13047
Previous Catalogue Name: Streptoccous mitis
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS904737
16S rRNA Gene Sequence: >gb|AF003929|ATCC 49456|Streptococcus mitis 16S ribosomal RNA gene, complete sequence.| ttttgagtttgatcc... >gb|D38482|NCTC12261|Streptococcus mitis 16S rRNA gene.| agagtttgatcatgg... >gb|DQ303188|ATCC 49456|Streptococcus mitis strain ATCC 49456 16S ribosomal RNA gene,partial sequence.| tcagacgaacgctgg... >gb|AY347550|ATCC 49456|Streptococcus mitis strain ATCC 49456 16S ribosomal RNA gene,partial sequence; and 16S-23S ribosomal RNA intergenic spacer,complete sequence.| gtttgtaacacccga... >gb|AY485601|ATCC 49456| GCSS 1303|Streptococcus mitis strain GCSS 1303 16S small subunit ribosomalRNA gene, partial sequence.| gacgaacgctggcgg...
23S rRNA Gene Sequence: >gb|AB006128|NCTC12261|Streptococcus mitis gene for 23S rRNA, partial sequence, strainNCTC12261.| gttggcttagaagca... >gb|AB006128|NCTC12261|Streptococcus mitis gene for 23S rRNA, partial sequence, strain:NCTC12261.| gttggcttagaagca... >gb|AY347550|ATCC 49456|Streptococcus mitis strain ATCC 49456 16S ribosomal RNA gene,partial sequence; and 16S-23S ribosomal RNA intergenic spacer,complete sequence.| gtttgtaacacccga... >gb|AY485601|ATCC 49456| GCSS 1303|Streptococcus mitis strain GCSS 1303 16S small subunit ribosomalRNA gene, partial sequence.| gacgaacgctggcgg...
Miscellaneous Sequence Data: >gb|AJ617790|DSM 12643|Streptococcus mitis partial gyrB gene for DNA gyrase subunit B.| gtcaacttcaaaaga... >gb|AF079215|NCTC12261|Streptococcus mitis strain NCTC12261 DNA gyrase subunit B (gyrB)gene, partial cds.| tcgtgtggctgccaa...
Extended Bibliography: showhide Show bibliography
Ref #: 95527
Author(s): Kawamura,Y.;Hou,X.G.;Sultana,F.;Miura,H.;Ezaki,T.
Journal: Int J Syst Bacteriol
Title: Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus
Volume: 45
Page(s): 406-8
Year: 1995
Keyword(s): GENBANK/D38482 GENBANK/D38483 DNA, Bacterial/*genetics Molecular Sequence Data Phylogeny RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Streptococcus/*classification/genetics
Remarks: We determined the 16S rRNA sequences of the type strains of Streptococcus mitis and Streptococcus gordonii and calculated the phylogenetic distances between those organisms and other members of the genus Streptococcus. The viridans group streptococci were separated into five phylogenetic groups; we named these groups the anginosus group, the mitis group, the salivarius group, the bovis group, and the mutans group. S. mitis and S. gordonii clustered in the mitis group together with Streptococcus pneumoniae, Streptococcus oralis, Streptococcus sanguis, and Streptococcus parasanguis at levels of sequence homology of more than 96%. Within this group, S. mitis, S. oralis, and S. pneumoniae exhibited more than 99% sequence homology with each other, although the DNA-DNA similarity values for their total chromosome DNAs were less than 60%.
URL: 7537076
Ref #: 95506
Author(s): Gonzalez,I.;Georgiou,M.;Alcaide,F.;Balas,D.;Linares,J.;de la Campa,A.G.
Journal: Antimicrob Agents Chemother
Title: Fluoroquinolone resistance mutations in the parC, parE, and gyrA genes of clinical isolates of viridans group streptococci
Volume: 42
Page(s): 2792-8
Year: 1998
Keyword(s): Amino Acid Sequence Anti-Infective Agents/*pharmacology Base Sequence Ciprofloxacin/pharmacology DNA Gyrase DNA Topoisomerase IV DNA Topoisomerases, Type II/drug effects/*genetics Drug Resistance, Microbial *Genes, Bacterial Humans Molecular Sequence Data Mutation Polymerase Chain Reaction Streptococcus/*drug effects/genetics Transformation, Genetic
Remarks: The nucleotide sequences of the quinolone resistance-determining regions (QRDRs) of the parC and gyrA genes from seven ciprofloxacin-resistant (Cpr) isolates of viridans group streptococci (two high-level Cpr Streptococcus oralis and five low-level Cpr Streptococcus mitis isolates) were determined and compared with those obtained from susceptible isolates. The nucleotide sequences of the QRDRs of the parE and gyrB genes from the five low-level Cpr S. mitis isolates and from the NCTC 12261 type strain were also analyzed. Four of these low-level Cpr isolates had changes affecting the subunits of DNA topoisomerase IV: three in Ser-79 (to Phe or Ile) of ParC and one in ParE at a position not previously described to be involved in quinolone resistance (Pro-424). One isolate did not show any mutation. The two high-level Cpr S. oralis isolates showed mutations affecting equivalent residue positions of ParC and GyrA, namely, Ser-79 to Phe and Ser-81 to Phe or Tyr, respectively. The parC mutations were able to transform Streptococcus pneumoniae to ciprofloxacin resistance, while the gyrA mutations transformed S. pneumoniae only when mutations in parC were present. These results suggest that DNA topoisomerase IV is a primary target of ciprofloxacin in viridans group streptococci, DNA gyrase being a secondary target.
URL: 9797205
Ref #: 39176
Author(s): Roth,S.B.;Jalava,J.;Ruuskanen,O.;Ruohola,A.;Nikkari,S.
Journal: J Clin Microbiol
Title: Use of an oligonucleotide array for laboratory diagnosis of bacteria responsible for acute upper respiratory infections
Volume: 42
Page(s): 4268-74
Year: 2004
Keyword(s): Bacterial Infections/*diagnosis Base Sequence DNA Primers DNA Topoisomerases, Type I/genetics Humans Oligonucleotide Array Sequence Analysis/*methods Oligonucleotide Probes Polymerase Chain Reaction Respiratory Tract Infections/*diagnosis Sensitivity and Specificity
Remarks: We developed a diagnostic array of oligonucleotide probes targeting species-specific variable regions of the genes encoding topoisomerases GyrB and ParE of respiratory bacterial pathogens. Suitable broad-range primer sequences were designed based on alignment of gyrB/parE sequences from nine different bacterial species. These species included Corynebacterium diphtheriae, Fusobacterium necrophorum, Haemophilus influenzae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus pyogenes. Specific probe sequences were selected by comparative analysis against the European Bioinformatics Database, as well as gyrB/parE sequences generated for this study. To verify specificity, at least six initial oligonucleotide probe sequences per bacterial species were tested by hybridization on a solid glass support using culture collection strains as templates. Finally, three oligonucleotide probes per bacterial species were utilized to examine 65 middle ear fluid and 29 throat swab samples. The sensitivities of the developed assay compared to classic culture from middle ear fluid samples for H. influenzae, M. catarrhalis, and S. pneumoniae were 96 (93 for culture), 73 (93 for culture), and 100% (78% for culture), respectively. No cross-reactivity with bacterial species belonging to the normal oral flora was observed when the 29 throat swab samples were studied. The sensitivity of the assay to detect S. pyogenes from these samples was 93% (80% for culture). These results provide a proof of concept for the diagnostic use of microarray technology based on broad-range topoisomerase gene amplification, followed by hybridization and specific detection of bacterial species.
URL: 15365022
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 84110
Author(s): Bekal,S.;Gaudreau,C.;Laurence,R.A.;Simoneau,E.;Raynal,L.
Journal: J Clin Microbiol
Title: Streptococcus pseudoporcinus sp. nov., a novel species isolated from the genitourinary tract of women
Volume: 44
Page(s): 2584-6
Year: 2006
Keyword(s): GENBANK/DQ303183 GENBANK/DQ303184 GENBANK/DQ303185 GENBANK/DQ303186 GENBANK/DQ303187 GENBANK/DQ303188 GENBANK/DQ303189 GENBANK/DQ303190 GENBANK/DQ303191 GENBANK/DQ303192 GENBANK/DQ303193 GENBANK/DQ303194 GENBANK/DQ303195 GENBANK/DQ303196 GENBANK/DQ303197 GENBANK/DQ303198 GENBANK/DQ303199 GENBANK/DQ303200 GENBANK/DQ303201 GENBANK/DQ303202 GENBANK/DQ303203 GENBANK/DQ303204 GENBANK/DQ303205 GENBANK/DQ303206 GENBANK/DQ303207 GENBANK/DQ303208 GENBANK/DQ303209 GENBANK/DQ340843 Cluster Analysis DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Female Female Urogenital Diseases/*microbiology Genes, rRNA Humans Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Streptococcal Infections/*microbiology Streptococcus/*classification/genetics/*isolation & purification Urogenital System/*microbiology
Remarks: Streptococcus strains from animal and human sources identified biochemically as Streptococcus porcinus were investigated by 16S rRNA gene sequencing. The nine human strains isolated between 1997 and 2005 formed a single cluster with more than 2.1% dissimilarity with S. porcinus strains from animal sources. A novel species, Streptococcus pseudoporcinus sp. nov., is proposed.
URL: 16825387
Ref #: 95446
Author(s): Sultana,F.;Kawamura,Y.;Hou,X.G.;Shu,S.E.;Ezaki,T.
Journal: FEMS Microbiol Lett
Title: Determination of 23S rRNA sequences from members of the genus Streptococcus and characterization of genetically distinct organisms previously identified as members of the Streptococcus anginosus group
Volume: 158
Page(s): 223-30
Year: 1998
Keyword(s): GENBANK/AB006119 GENBANK/AB006120 GENBANK/AB006121 GENBANK/AB006122 GENBANK/AB006123 GENBANK/AB006124 GENBANK/AB006125 GENBANK/AB006126 GENBANK/AB006127 GENBANK/AB006128 GENBANK/AB006129 GENBANK/D63800 GENBANK/D63801 GENBANK/D63802 GENBANK/D63803 GENBANK/D63804 GENBANK/D63805 GENBANK/D63806 Base Sequence Classification Molecular Sequence Data Oligonucleotide Probes Phylogeny RNA, Bacterial/analysis RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, RNA Species Specificity Streptococcus/*classification/*genetics
Remarks: The 23S rRNA gene sequences of eight type strains of the genus Streptococcus were determined. Three species-specific probes for the three members of the Streptococcus anginosus group and one group-specific probe for these three species were designed. The PCR-amplified 23S rRNA gene sequences of 28 clinical strains were examined by hybridization with the four oligonucleotide probes. They were suspected to be members of the S. anginosus group by biochemical tests; however, only 21 strains (75%) hybridized to one of the three species probes. Seven biochemically atypical strains did not hybridize to these species-specific probes. Of the seven strains, three hybridized to the group-specific probe. The 23S ribosomal RNA sequences of these three strains differed by three bases within the 20-base probe area of S. anginosus. The phylogenetic tree of the 16S rRNA of these three strains located them within the branch of S. anginosus. The remaining four strains, which did not hybridize to group- or species-specific probes, had a raffinose-positive and VP-negative phenotype. The probe hybridization results showed that these four strains did not belong to the S. anginosus group and were closely related to S. parasanguis according to partial sequences of their 16S rRNA genes.
URL: 9465395
Ref #: 13406
Author(s): Kawamura,Y.;Hou,X.G.;Sultana,F.;Miura,H.;Ezaki,T.
Journal: Int J Syst Bacteriol
Title: Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus
Volume: 45
Page(s): 406-8
Year: 1995
Keyword(s): GENBANK/D38482 GENBANK/D38483 DNA, Bacterial/*genetics Molecular Sequence Data Phylogeny RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Streptococcus/*classification/genetics
Remarks: We determined the 16S rRNA sequences of the type strains of Streptococcus mitis and Streptococcus gordonii and calculated the phylogenetic distances between those organisms and other members of the genus Streptococcus. The viridans group streptococci were separated into five phylogenetic groups; we named these groups the anginosus group, the mitis group, the salivarius group, the bovis group, and the mutans group. S. mitis and S. gordonii clustered in the mitis group together with Streptococcus pneumoniae, Streptococcus oralis, Streptococcus sanguis, and Streptococcus parasanguis at levels of sequence homology of more than 96%. Within this group, S. mitis, S. oralis, and S. pneumoniae exhibited more than 99% sequence homology with each other, although the DNA-DNA similarity values for their total chromosome DNAs were less than 60%.
URL: 95244327
Ref #: 13392
Author(s): Sultana,F.;Kawamura,Y.;Hou,X.G.;Shu,S.E.;Ezaki,T.
Journal: FEMS Microbiol Lett
Title: Determination of 23S rRNA sequences from members of the genus Streptococcus and characterization of genetically distinct organisms previously identified as members of the Streptococcus anginosus group
Volume: 158
Page(s): 223-30
Year: 1998
Keyword(s): GENBANK/AB006119 GENBANK/AB006120 GENBANK/AB006121 GENBANK/AB006122 GENBANK/AB006123 GENBANK/AB006124 GENBANK/AB006125 GENBANK/AB006126 GENBANK/AB006127 GENBANK/AB006128 GENBANK/AB006129 GENBANK/D63800 GENBANK/D63801 GENBANK/D63802 GENBANK/D63803 GENBANK/D63804 GENBANK/D63805 GENBANK/D63806 Base Sequence Classification Molecular Sequence Data Oligonucleotide Probes Phylogeny RNA, Bacterial/analysis RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, RNA Species Specificity Streptococcus/*classification/*genetics Support, Non-U.S. Gov't
Remarks: The 23S rRNA gene sequences of eight type strains of the genus Streptococcus were determined. Three species-specific probes for the three members of the Streptococcus anginosus group and one group-specific probe for these three species were designed. The PCR-amplified 23S rRNA gene sequences of 28 clinical strains were examined by hybridization with the four oligonucleotide probes. They were suspected to be members of the S. anginosus group by biochemical tests; however, only 21 strains (75%) hybridized to one of the three species probes. Seven biochemically atypical strains did not hybridize to these species-specific probes. Of the seven strains, three hybridized to the group-specific probe. The 23S ribosomal RNA sequences of these three strains differed by three bases within the 20-base probe area of S. anginosus. The phylogenetic tree of the 16S rRNA of these three strains located them within the branch of S. anginosus. The remaining four strains, which did not hybridize to group- or species-specific probes, had a raffinose-positive and VP-negative phenotype. The probe hybridization results showed that these four strains did not belong to the S. anginosus group and were closely related to S. parasanguis according to partial sequences of their 16S rRNA genes.
URL: 98126543
Ref #: 3965
Author(s): Kilian,M.;Mikkelsen,L.;Henrichsen,J.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomic study of viridans streptococci: Description of Streptococcus gordonii sp. nov. and emended descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrewes and Horder
Volume: 39
Page(s): 471-484
Year: 1989
Ref #: 7334
Author(s): Coykendall,A.L.;Munzenmaier,A.J.
Journal: Int. J. Syst. Bacteriol.
Title: Deoxyribonucleic acid base sequence studies on glucan-producing and glucan-negative strains of Streptococcus mitior.
Volume: 28
Page(s): 511-515
Year: 1978
Ref #: 7335
Author(s): Kilian,M.;Mikkelsen,L.;Henrichsen,J.
Journal: Int. J. Syst. Bacteriol.
Title: Replacement of the type strain of Streptococcus mitis. Request for an opinion.
Volume: 39
Page(s): 498-499
Year: 1989
Ref #: 7336
Journal: Int. J. Syst. Bacteriol.
Title: Opinion 66. Designation of strain NS 51 (NCTC 12261) in place of strain NCTC 3165 as the type strain of Streptococcus mitis Andrews and Horder 1906.
Volume: 43
Page(s): 391
Year: 1993
Data: (ATCC 49456) Proposed type strain/M. Kilian, Aarhus, Denmark in 1989/J. Carlsson/Oral cavity, human/Kilian, M. et al. (1989) Int. J.syst.Bact. 39, 471/Kilian, M. et al. (1989) Int.J.syst.Bact. 39, 498
Accession Date: 04/02/2003
Authority: Andrewes & Horder 1906
Taxonomy: TaxLink: ? (NCTC 12261) - Date of change: 04/02/2003
Other: see also Streptococcus sp. Viridans type
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

Note: Links open in a new window

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.