Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Campylobacter hominis

NCTC Number: NCTC 13146
Current Name: Campylobacter hominis
Other Collection No: CH001; CIP 107682; DSM 12671; LMG 19568
Type Strain: Yes
Family: Campylobacteraceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
16S rRNA Gene Sequence: >gb|DQ174182|NCTC 13146|Campylobacter hominis strain NCTC 13146 16S ribosomal RNA (rrs)gene, partial sequence.| agtgaacgctggcgg... >gb|AJ251584|NCTC 13146|CH001A|Campylobacter hominis partial 16S rRNA gene, strain NCTC 13146.| aacgctggcggcgtg...
Extended Bibliography: showhide Show bibliography
Ref #: 32077
Author(s): Lawson,A.J.;On,S.L.;Logan,J.M.;Stanley,J.
Journal: Int J Syst Evol Microbiol
Title: Campylobacter hominis sp. nov., from the human gastrointestinal tract
Volume: 51
Page(s): 651-60
Year: 2001
Keyword(s): GENBANK/AJ251584 Bacterial Typing Techniques Base Composition Campylobacter/*classification/isolation & purification DNA, Ribosomal/genetics Diarrhea/microbiology Feces/*microbiology Humans Intestines/*microbiology Molecular Sequence Data Nucleic Acid Hybridization Phenotype RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Terminology
Remarks: Sequences of 16S rDNA of a novel campylobacter from faeces of healthy humans were previously shown to originate from a new taxon, 'Candidatus Campylobacter hominis', which could not be cultured. Since phylogenetic analysis suggested that anaerobic conditions might be required for growth, an isolation strategy was developed employing initial non-selective membrane filtration onto fastidious anaerobe agar. Campylobacters were then isolated from the resulting mixed microbial flora by a dilution strategy and/or by immunomagnetic separation with genus-specific polyclonal antibody. Isolates were identified by a genus and taxon-specific PCR assay, and 16S rDNA nucleotide sequence analysis was carried out. All isolates exhibited the typical Campylobacter characteristics of being non-fermentative, oxidase-positive, catalase-negative and Gram-negative. Unusually, however, they were straight rods lacking flagella. The 16S rDNA nucleotide sequence analysis, DNA and mol% G+C were consistent with a new Campylobacter species whose nearest phylogenetic neighbours were Campylobacter gracilis and Campylobacter sputorum. The unique species status of the isolates was further confirmed by taxonomic analysis of 47 phenotypic characteristics. The name Campylobacter hominis sp. nov. is proposed for the new species, the type strain of which is NCTC 13146T (= LMG 19568T).
URL: 11321111
Ref #: 95450
Author(s): Korczak,B.M.;Stieber,R.;Emler,S.;Burnens,A.P.;Frey,J.;Kuhnert,P.
Journal: Int J Syst Evol Microbiol
Title: Genetic relatedness within the genus Campylobacter inferred from rpoB sequences
Volume: 56
Page(s): 937-45
Year: 2006
Keyword(s): Bacterial Proteins/*genetics Base Sequence Campylobacter/*classification/*genetics DNA Primers DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics DNA-Directed RNA Polymerases/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Statistics
Remarks: The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.
URL: 16627635
Accession Date: 14/06/2007
Taxonomy: TaxLink: S7123 (Campylobacter hominis lawson et al. 2001) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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Available Formats

Ampoule (Bacteria)
Bacterial DNA - 2µg

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