Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Streptococcus uberis

NCTC Number: NCTC 3858
Current Name: Streptococcus uberis
Original Strain Reference: Diernhofer
Other Collection No: ATCC 19436; CCUG 17930; DSM 20569; JCM 5709; NCDO 2038
Previous Catalogue Name: Streptococcus uberis
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: not recorded
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1058914
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AB002526|ATCC 19436| NCTC 3858|Streptococcus uberis DNA for 16S rRNA, strain ATCC 19436, NCTC3858.| gaggactggtgctgc... >gb|AF255657|NCDO 2038|Streptococcus uberis 16S-23S ribosomal RNA intergenic spacer,complete sequence, 23S ribosomal RNA partial sequence.| acccgaagcggtgag... >gb|AY347538|ATCC 19436|Streptococcus uberis strain ATCC 19436 16S-23S ribosomal RNAintergenic spacer, complete sequence.| aaggataaggaacac...
23S rRNA Gene Sequence: >gb|AF255657|NCDO 2038|Streptococcus uberis 16S-23S ribosomal RNA intergenic spacer,complete sequence, 23S ribosomal RNA partial sequence.| acccgaagcggtgag... >gb|X68038 S60799|NCDO 2038|S.uberis gene for 23S rRNA.| ggttaagttaataag... >gb|AY347538|ATCC 19436|Streptococcus uberis strain ATCC 19436 16S-23S ribosomal RNAintergenic spacer, complete sequence.| aaggataaggaacac...
Bibliography: DIERNHOFER K 1932 MICHW.FORSCH. 13 368
Extended Bibliography: showhide Show bibliography
Ref #: 84000
Author(s): Hassan,A.A.;Khan,I.U.;Abdulmawjood,A.;Lammler,C.
Journal: J Clin Microbiol
Title: Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis
Volume: 39
Page(s): 1618-21
Year: 2001
Keyword(s): GENBANK/AF255656 GENBANK/AF255657 Animals Base Sequence Cattle Cattle Diseases/microbiology DNA, Ribosomal Spacer/*genetics *Genes, rRNA Mastitis, Bovine/*microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sequence Analysis, DNA Streptococcal Infections/microbiology/*veterinary Streptococcus/*classification/genetics Time Factors
Remarks: Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.
URL: 11283100
Ref #: 84005
Author(s): Harland,N.M.;Leigh,J.A.;Collins,M.D.
Journal: J Appl Bacteriol
Title: Development of gene probes for the specific identification of Streptococcus uberis and Streptococcus parauberis based upon large subunit rRNA gene sequences
Volume: 74
Page(s): 526-31
Year: 1993
Keyword(s): Bacterial Typing Techniques Base Sequence DNA, Bacterial/genetics DNA, Ribosomal/*genetics Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization *Oligonucleotide Probes Polymerase Chain Reaction RNA, Bacterial/*genetics RNA, Ribosomal, 23S/*genetics Streptococcus/*classification/genetics
Remarks: 23S ribosomal RNa (rRNA) gene sequences of Streptococcus uberis and Strep. parauberis were determined by direct polymerase chain reaction (PCR) sequencing. Oligonucleotide probes specific for Strep. uberis and Strep. parauberis were designed from variable regions of the 23S rRNA gene sequence data. Molecular hybridizations with PCR-amplified rRNA gene targets provided a precise and reliable means of differentiating Strep. uberis and Strep. parauberis from each other and from other streptococcal species.
URL: 7683638
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 13418
Author(s): Harland,N.M.;Leigh,J.A.;Collins,M.D.
Journal: J Appl Bacteriol
Title: Development of gene probes for the specific identification of Streptococcus uberis and Streptococcus parauberis based upon large subunit rRNA gene sequences
Volume: 74
Page(s): 526-31
Year: 1993
Keyword(s): Bacterial Typing Techniques Base Sequence DNA, Bacterial/genetics DNA, Ribosomal/*genetics Genes, Bacterial Molecular Sequence Data Nucleic Acid Hybridization *Oligonucleotide Probes Polymerase Chain Reaction RNA, Bacterial/*genetics RNA, Ribosomal, 23S/*genetics Streptococcus/*classification/genetics Support, Non-U.S. Gov't
Remarks: 23S ribosomal RNa (rRNA) gene sequences of Streptococcus uberis and Strep. parauberis were determined by direct polymerase chain reaction (PCR) sequencing. Oligonucleotide probes specific for Strep. uberis and Strep. parauberis were designed from variable regions of the 23S rRNA gene sequence data. Molecular hybridizations with PCR-amplified rRNA gene targets provided a precise and reliable means of differentiating Strep. uberis and Strep. parauberis from each other and from other streptococcal species.
URL: 93252678
Ref #: 13427
Author(s): Hassan,A.A.;Khan,I.U.;Abdulmawjood,A.;Lammler,C.
Journal: J Clin Microbiol
Title: Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis
Volume: 39
Page(s): 1618-21
Year: 2001
Keyword(s): GENBANK/AF255656 GENBANK/AF255657 Animal Base Sequence Cattle Cattle Diseases/microbiology DNA, Ribosomal Spacer/*genetics *Genes, rRNA Mastitis, Bovine/*microbiology Molecular Sequence Data Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sequence Analysis, DNA Streptococcal Infections/microbiology/*veterinary Streptococcus/*classification/genetics Time Factors
Remarks: Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.
URL: 21179298
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 3220
Author(s): Collins,M.D.;Farrow,J.A.E.;Katic,V.;Kandler,O.
Journal: System. Appl. Microbiol.
Title: Taxonomic studies on streptococci of serological groups E, P, U, and V: description of Streptococcus porcinus sp. nov.
Volume: 5
Page(s): 402-413
Year: 1984
Ref #: 3345
Author(s): Kilpper-Bälz,R.;Wenzig,P.;Schleifer,K.H.
Journal: Int. J. Syst. Bacteriol.
Title: Molecular relationships and classification of some viridans streptococci as Streptococcus oralis and emended description of Streptococcus oralis (Bridge and Sneath 1982).
Volume: 35
Page(s): 482-488
Year: 1985
Data: (ATCC 19436, NCDO 2038) Type strain / K. Diernhofer, Vienna in 1933 / Diernhofer, K. (1932) Milchw. Forsch. 13, 368
Accession Date: 01/01/1933
History: FROM DIERNHOFER K ,TIERARTZLICHE HOCHSCHULE ,VIENNA
Authority: Diernhofer 1932 (AL)
Depositor: DIERNHOFER K
Taxonomy: TaxLink: S2870 (Streptococcus uberis Diernhofer 1932) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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