Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Yersinia pestis

NCTC Number: NCTC 5923
Current Name: Yersinia pestis
Original Strain Reference: Soemedang
Other Collection No: ATCC 19428; SOEMEDANG
Previous Catalogue Name: Yersinia pestis
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 3
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: nutrient agar,37, facultative anaerobe
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: javan strain
16S rRNA Gene Sequence: >gb|AF366383|NCTC 5923|Yersinia pestis 16S ribosomal RNA gene, partial sequence.| attgaacgctggcgg...
Bibliography: SCHUTZE H 1939 BRIT J EXP PATH 20 235
Extended Bibliography: showhide Show bibliography
Ref #: 95482
Author(s): Kim,W.;Song,M.O.;Song,W.;Kim,K.J.;Chung,S.I.;Choi,C.S.;Park,Y.H.
Journal: Antonie Van Leeuwenhoek
Title: Comparison of 16S rDNA analysis and rep-PCR genomic fingerprinting for molecular identification of Yersinia pseudotuberculosis
Volume: 83
Page(s): 125-33
Year: 2003
Keyword(s): GENBANK/AF365934 GENBANK/AF365935 GENBANK/AF365936 GENBANK/AF365937 GENBANK/AF365938 GENBANK/AF365939 GENBANK/AF365940 GENBANK/AF365941 GENBANK/AF365942 GENBANK/AF365943 GENBANK/AF365944 GENBANK/AF365945 GENBANK/AF365946 GENBANK/AF365947 GENBANK/AF365948 GENBANK/AF365949 GENBANK/AF365950 GENBANK/AF366375 GENBANK/AF366376 GENBANK/AF366377 GENBANK/AF366378 GENBANK/AF366379 GENBANK/AF366380 GENBANK/AF366381 GENBANK/AF366382 GENBANK/AF366383 GENBANK/AF366384 GENBANK/AF366385 DNA Fingerprinting/*methods DNA, Bacterial/analysis DNA, Ribosomal/analysis Genome, Bacterial Humans Molecular Sequence Data Phylogeny Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/*genetics *Sequence Analysis, DNA Yersinia pestis/classification/genetics Yersinia pseudotuberculosis/*classification/genetics
Remarks: 16S rDNA sequence analysis and repetitive element sequence-based PCR (rep-PCR) genomic fingerprinting were evaluated on 11 type strains of the genus Yersinia and 17 recognized serotype strains of Y. pseudotuberculosis to investigate their genetic relatedness and to establish the value of techniques for the identification of Y. pseudotuberculosis. A phylogenetic tree constructed from 16S rDNA sequences showed that the type strains of Yersinia species formed distinct clusters with the exception of Y. pestis and Y. pseudotuberculosis. Moreover, Y. pestis NCTC 5923T was found to be closely related to Y. pseudotuberculosis serotypes 1b, 3, and 7. Dendrograms generated from REP-PCR, and ERIC-PCR data revealed that members of the genus Yersinia differed from each other with the degree of similarity 62% and 58%, respectively. However, the BOX-PCR results showed that Y. pestis 5923T clustered with the Y. pseudotuberculosis group with a degree of similarity 74%. According to these findings, 16S rDNA sequence analysis was unable to reliably discriminate Y. pseudotuberculosis from Y. pestis. However, REP-PCR and especially ERIC-PCR provided an effective means of differentiating between members of the taxa.
URL: 12785306
Data: (ATCC 19428) Type strain / H. Schutze, London in 1939 / Javan strain, avirulent / Vaccine production / Schutze, H. (1939) Br. J. exp. Path. 20, 235
Accession Date: 01/01/1939
History: SCHUTZE H,LISTER INSTITUTE ,LONDON
Authority: (Lehmann and Neumann 1896) van Loghem 1944 (AL)
Depositor: SCHUTZE H
Taxonomy: TaxLink: S3616 (Yersinia pestis (Lehmann and Neumann 1896) van Loghem 1944) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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Available Formats

Ampoule (Bacteria)
Bacterial DNA - 2µg

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