Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Enterococcus faecium

NCTC Number: NCTC 7171
Current Name: Enterococcus faecium
Original Strain Reference: Grumbach serotype 11
Other Collection No: ATCC 19434; CCUG 542; CFBP 4248; CIP 103014; DSM 20477; GRUMBACH SEROTYPE 11; HAMBI 1710; JCM 5804; JCM 8727; LMG 11423; NBRC 100485; NBRC 100486; NCIMB 11508; WDCM 00010
Previous Catalogue Name: Enterococcus faecium
Type Strain: Yes
Family: Enterococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group d, serotype 11
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1043813
16S rRNA Gene Sequence: >gb|AJ276355|DSM20477|Enterococcus faecium 16S rRNA gene, strain DSM20477.| tggctcaggacgaac... >gb|X87180|ATCC 19434|E.faecium 16S-23S rRNA spacer DNA, strain ATCC 19434.| ctaaggaatattacg... >gb|DQ411813|ATCC 19434|Enterococcus faecium strain ATCC 19434 16S ribosomal RNA gene,partial sequence.| gacgaacgctggcgg... >gb|AY351322|ATCC 19434|Enterococcus faecium strain ATCC 19434 16S-23S ribosomal RNAintergenic spacer, partial sequence.| ctaaggaatattacg...
23S rRNA Gene Sequence: >gb|AF273486|ATCC19434|Enterococcus faecium 23S ribosomal RNA gene, partial sequence.| gtagtttgatattga... >gb|X87180|ATCC 19434|E.faecium 16S-23S rRNA spacer DNA, strain ATCC 19434.| ctaaggaatattacg... >gb|AY351322|ATCC 19434|Enterococcus faecium strain ATCC 19434 16S-23S ribosomal RNAintergenic spacer, partial sequence.| ctaaggaatattacg...
Extended Bibliography: showhide Show bibliography
Ref #: 43380
Author(s): Tsiodras,S.;Gold,H.S.;Coakley,E.P.;Wennersten,C.;Moellering RC,J.r.;Eliopoulos,G.M.
Journal: J Clin Microbiol
Title: Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species
Volume: 38
Page(s): 3991-3
Year: 2000
Keyword(s): Animals Enterococcus/*genetics/isolation & purification Genes, Bacterial Genes, rRNA/*genetics Gram-Positive Bacterial Infections/*microbiology Humans Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 23S/chemistry/*genetics Sequence Analysis, DNA *Variation (Genetics)
Remarks: The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species of Enterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosus, Enterococcus mundtii, Enterococcus malodoratus, and Enterococcus hirae (one isolate of each). For all isolates, species identification by biochemical testing was corroborated by 16S rRNA gene sequencing. The sequence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all other enterococci. The domain V sequences of E. durans and E. hirae were identical. This was also true for E. gallinarum and E. casseliflavus. E. avium differed from E. casseliflavus by 23 bases, from E. durans by 16 bases, and from E. malodoratus by 2 bases. E. avium differed from E. raffinosus by one base. Despite the fact that domain V is considered to be highly conserved, substantial differences were identified between several enterococcal species.
URL: 11060057
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 43856
Author(s): Naimi,A.;Beck,G.;Branlant,C.
Journal: Microbiology
Title: Primary and secondary structures of rRNA spacer regions in enterococci
Volume: 143 ( Pt 3)
Page(s): 823-34
Year: 1997
Keyword(s): GENBANK/X87177 GENBANK/X87178 GENBANK/X87179 GENBANK/X87180 GENBANK/X87181 GENBANK/X87182 GENBANK/X87183 GENBANK/X87184 GENBANK/X87185 GENBANK/X87186 GENBANK/X87187 GENBANK/X87188 GENBANK/X87189 GENBANK/X87190 GENBANK/X87191 Base Sequence Enterococcus/*genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial/chemistry/*genetics RNA, Ribosomal/chemistry/*genetics RNA, Transfer/genetics Sequence Alignment
Remarks: The 16S-23S and 23S-5S rRNA spacer DNA regions (spacer regions 1 and 2, respectively) from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans and Enterococcus mundtii were amplified by PCR. Their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. Whereas lactococci and Streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high degree of variability in this region; thus the spacer regions 1 with and without tRNA(Ala) were characterized. However, as shown for lactococci and Streptococcus sensu stricto, the tRNA(Ala) gene does not encode the 3'-terminal CCA trinucleotide. A putative antitermination signal is found downstream from the tRNA(Ala) gene. Based on comparison with Lactococcus lactis and Streptococcus thermophilus, a double-stranded processing stem is proposed. In E, hirae, one of the three different types of spacer region 1 contains no tRNA(Ala), but displays a 107 nt insertion that forms a long stem-loop structure. A similar insertion (115 nt in length) was found in E. faecium and base compensatory mutations preserve the ability to form the long stem-loop structure. Such insertions may correspond to mobile intervening sequences, as found in the 23S rRNA coding sequences of some Gram-negative bacteria. The spacer regions 1 and 2 from the three subgroups of streptococci were compared, and except for the tRNA(Ala) gene and the double-stranded processing sites, little similarity was found, which opens large possibilities for future development of DNA-based typing methods.
URL: 9084166
Ref #: 13719
Author(s): Naimi,A.;Beck,G.;Branlant,C.
Journal: Microbiology
Title: Primary and secondary structures of rRNA spacer regions in enterococci
Volume: 143 ( Pt 3)
Page(s): 823-34
Year: 1997
Keyword(s): GENBANK/X87177 GENBANK/X87178 GENBANK/X87179 GENBANK/X87180 GENBANK/X87181 GENBANK/X87182 GENBANK/X87183 GENBANK/X87184 GENBANK/X87185 GENBANK/X87186 GENBANK/X87187 GENBANK/X87188 GENBANK/X87189 GENBANK/X87190 GENBANK/X87191 Base Sequence Enterococcus/*genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial/chemistry/*genetics RNA, Ribosomal/chemistry/*genetics RNA, Transfer/genetics Sequence Alignment Support, Non-U.S. Gov't
Remarks: The 16S-23S and 23S-5S rRNA spacer DNA regions (spacer regions 1 and 2, respectively) from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans and Enterococcus mundtii were amplified by PCR. Their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. Whereas lactococci and Streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high degree of variability in this region; thus the spacer regions 1 with and without tRNA(Ala) were characterized. However, as shown for lactococci and Streptococcus sensu stricto, the tRNA(Ala) gene does not encode the 3'-terminal CCA trinucleotide. A putative antitermination signal is found downstream from the tRNA(Ala) gene. Based on comparison with Lactococcus lactis and Streptococcus thermophilus, a double-stranded processing stem is proposed. In E, hirae, one of the three different types of spacer region 1 contains no tRNA(Ala), but displays a 107 nt insertion that forms a long stem-loop structure. A similar insertion (115 nt in length) was found in E. faecium and base compensatory mutations preserve the ability to form the long stem-loop structure. Such insertions may correspond to mobile intervening sequences, as found in the 23S rRNA coding sequences of some Gram-negative bacteria. The spacer regions 1 and 2 from the three subgroups of streptococci were compared, and except for the tRNA(Ala) gene and the double-stranded processing sites, little similarity was found, which opens large possibilities for future development of DNA-based typing methods.
URL: 97237711
Ref #: 13705
Author(s): Tsiodras,S.;Gold,H.S.;Coakley,E.P.;Wennersten,C.;Moellering RC,J.r.;Eliopoulos,G.M.
Journal: J Clin Microbiol
Title: Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species
Volume: 38
Page(s): 3991-3
Year: 2000
Keyword(s): Animal Enterococcus/*genetics/isolation & purification Genes, Bacterial Genes, rRNA/*genetics Gram-Positive Bacterial Infections/*microbiology Human Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 23S/chemistry/*genetics Sequence Analysis, DNA *Variation (Genetics)
Remarks: The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species of Enterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosus, Enterococcus mundtii, Enterococcus malodoratus, and Enterococcus hirae (one isolate of each). For all isolates, species identification by biochemical testing was corroborated by 16S rRNA gene sequencing. The sequence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all other enterococci. The domain V sequences of E. durans and E. hirae were identical. This was also true for E. gallinarum and E. casseliflavus. E. avium differed from E. casseliflavus by 23 bases, from E. durans by 16 bases, and from E. malodoratus by 2 bases. E. avium differed from E. raffinosus by one base. Despite the fact that domain V is considered to be highly conserved, substantial differences were identified between several enterococcal species.
URL: 20514229
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 2392
Author(s): Ottogalli,G.;Galli,A.;Dellaglio,F.
Journal: J. Dairy Res.
Title: Taxonomic relationships between Streptococcus thermophilus and some other streptococci.
Volume: 46
Page(s): 127-131
Year: 1979
Ref #: 2681
Author(s): Schleifer,K.H.;Kilpper-Bälz,R.
Journal: Int. J. Syst. Bacteriol.
Title: Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov.
Volume: 34
Page(s): 31-34
Year: 1984
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (ATCC 19434, NCDO 942, NCIB 11508) Type strain / A. Grumbach, Zurich in 1946
Accession Date: 01/01/1946
History: GRUMBACH A ZURICH
Authority: (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984
Depositor: GRUMBBACH A
Taxonomy: TaxLink: S1145 (Streptococcus faecium) - Date of change: 5/02/2003
Other: Group D Grumbach Serotype. Type 11Part of a set of strains deposited by A. Grumbach, Zurich in 1946.
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

Note: Links open in a new window

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.