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Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Bacteroides fragilis

NCTC Number: NCTC 9343
Current Name: Bacteroides fragilis
Original Strain Reference: Onslow
Other Collection No: ATCC 25285; CN 6300; DSM 2151; ONSLOW
Previous Catalogue Name: Bacteroides fragilis
Other Names: BACTEROIDES FRAGILIS, (VEILLON AND ZUBER 1898) CASTELLANI AND CHALMERS
Type Strain: Yes
Family: Bacteroidaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Blood agar (Fastidious anaerobe agar), 24-48 hours, 37°C, anaerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, apendix abscess
16S rRNA Gene Sequence: >gb|AB050106|DSM 2151T|Bacteroides fragilis gene for 16S rRNA, partial sequence.| gtttgatcatggctc... >gb|AF172709|ATCC25285|Bacteroides fragilis 16S-23S ribosomal RNA intergenic spacerregion, partial sequence.| ggagcgatgttgaaa... >gb|X83935|ATCC 25285T|@1|B.fragilis 16S rRNA (ATCC 25285T).| atgaacgctagctac...
23S rRNA Gene Sequence: >gb|AF172709|ATCC25285|Bacteroides fragilis 16S-23S ribosomal RNA intergenic spacerregion, partial sequence.| ggagcgatgttgaaa... >gb|AY650901|ATCC 25285|Bacteroides fragilis strain ATCC 25285 23S ribosomal RNA gene,partial sequence.| ggcattctgaaggaa... >gb|AY155459|ATCC 25285|Bacteroides fragilis 23S ribosomal RNA gene, partial sequence.| aagcagccattcatt...
Miscellaneous Sequence Data: >gb|AB048185|ATCC 25285|Bacteroides fragilis gyrB gene for DNA gyrase B subunit, partialcds.| gataaaggttcgtac...
Extended Bibliography: showhide Show bibliography
Ref #: 95538
Author(s): Ruimy,R.;Podglajen,I.;Breuil,J.;Christen,R.;Collatz,E.
Journal: J Bacteriol
Title: A recent fixation of cfiA genes in a monophyletic cluster of Bacteroides fragilis is correlated with the presence of multiple insertion elements
Volume: 178
Page(s): 1914-8
Year: 1996
Keyword(s): GENBANK/X83935 GENBANK/X83936 GENBANK/X83937 GENBANK/X83938 GENBANK/X83939 GENBANK/X83940 GENBANK/X83941 GENBANK/X83942 GENBANK/X83943 GENBANK/X83944 GENBANK/X83945 GENBANK/X83946 GENBANK/X83947 GENBANK/X83948 GENBANK/X83949 GENBANK/X83950 GENBANK/X83951 GENBANK/X83952 GENBANK/X83953 GENBANK/X83954 *Bacterial Proteins Bacteroides fragilis/*classification/*genetics Base Sequence *DNA Transposable Elements DNA, Bacterial DNA, Ribosomal Flavobacterium/classification/genetics Flexibacteraceae/classification/genetics *Genes, Bacterial Molecular Sequence Data Multigene Family Phylogeny beta-Lactamases/classification/*genetics
Remarks: Small-subunit ribosomal DNA sequences of 16 strains of Bacteroides fragilis were determined and compared with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods) as well as a bootstrap analysis were used to assess the robustness of each topology. All phylogenetic analyses demonstrated that the B. fragilis strains were clearly divided into two robust monophyletic units which corresponded to the cfiA-negative and cfiA-positive groups. Strains of two previously identified DNA homology groups separated similarly into the two monophyletic units. According to the intensity of the hybridization signal with a cfiA probe, the cfiA-positive cluster could be further divided into two groups. This difference might reflect the existence of two, probably closely related cfiA-type genes. In the strongly hybridizing cfiA-positive strains, the gene is capable of conferring high-level resistance to the carbapenems and to most beta-lactamase inhibitors as well, while in the weakly hybridizing cfiA-positive strains, only the latter type of resistance is known to occur. The presence of the cfiA-type genes within a monophyletic cluster of B. fragilis that apparently represents only a minority of the species B. fragilis is suggestive of a recent acquisition. The fact that this cluster is also the predominant pool of all known B. fragilis insertion elements, which have been found to play an important role in the expression of carbapenem resistance, raises the possibility that both genetic determinants, i.e., the resistance gene(s) and insertion elements, may have coevolved.
URL: 8606165
Ref #: 25269
Author(s): Liu,C.;Song,Y.;McTeague,M.;Vu,A.W.;Wexler,H.;Finegold,S.M.
Journal: FEMS Microbiol Lett
Title: Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers
Volume: 222
Page(s): 9-16
Year: 2003
Keyword(s): Bacteriological Techniques Bacteroides fragilis/*classification/*genetics/growth & development DNA Transposable Elements/genetics Phenotype Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics
Remarks: We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were first divided into three subgroups by multiplex PCR-G, followed by three multiplex PCR assays with three species-specific primer mixtures for identification to the species level. The primers were designed from nucleotide sequences of the 16S rRNA, the 16S-23S rRNA intergenic spacer region and part of the 23S rRNA gene. The established two-step multiplex PCR identification scheme was applied to the identification of 155 clinical isolates of the B. fragilis group that were previously identified to the species level by phenotypic tests. The new scheme was more accurate than phenotypic identification, which was accurate only 84.5% of the time. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species. This will permit more accurate assessment of the role of various B. fragilis group members in infections and of the degree of antimicrobial resistance in each of the group members.
URL: 12757940
Ref #: 25883
Author(s): Matsuo,Y.;Suzuki,M.;Kasai,H.;Shizuri,Y.;Harayama,S.
Journal: Environ Microbiol
Title: Isolation and phylogenetic characterization of bacteria capable of inducing differentiation in the green alga Monostroma oxyspermum
Volume: 5
Page(s): 25-35
Year: 2003
Keyword(s): GENBANK/AB073026 GENBANK/AB073027 GENBANK/AB073028 GENBANK/AB073029 GENBANK/AB073030 GENBANK/AB073031 GENBANK/AB073032 GENBANK/AB073033 GENBANK/AB073034 GENBANK/AB073035 GENBANK/AB073036 GENBANK/AB073037 GENBANK/AB073038 GENBANK/AB073039 GENBANK/AB073040 GENBANK/AB073041 GENBANK/AB073042 GENBANK/AB073043 GENBANK/AB073044 GENBANK/AB073045 GENBANK/AB073046 GENBANK/AB073047 GENBANK/AB073048 GENBANK/AB073049 GENBANK/AB073050 GENBANK/AB073051 GENBANK/AB073052 GENBANK/AB073053 GENBANK/AB073054 GENBANK/AB073055 GENBANK/AB073056 GENBANK/AB073057 GENBANK/AB073058 GENBANK/AB073059 GENBANK/AB073060 GENBANK/AB073061 GENBANK/AB073062 GENBANK/AB073063 GENBANK/AB073064 GENBANK/AB073065 GENBANK/AB073066 GENBANK/AB073067 GENBANK/AB073068 GENBANK/AB073069 GENBANK/AB073070 GENBANK/AB073071 GENBANK/AB073072 GENBANK/AB073073 GENBANK/AB073074 GENBANK/AB073075 GENBANK/AB073076 GENBANK/AB073077 GENBANK/AB073078 GENBANK/AB073079 GENBANK/AB073080 GENBANK/AB073081 GENBANK/AB073082 GENBANK/AB073083 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GENBANK/AB073548 GENBANK/AB073549 GENBANK/AB073550 GENBANK/AB073551 GENBANK/AB073552 GENBANK/AB073553 GENBANK/AB073554 GENBANK/AB073555 GENBANK/AB073556 GENBANK/AB073557 GENBANK/AB073558 GENBANK/AB073559 GENBANK/AB073560 GENBANK/AB073561 GENBANK/AB073562 GENBANK/AB073563 GENBANK/AB073564 GENBANK/AB073565 GENBANK/AB073566 GENBANK/AB073567 GENBANK/AB073568 GENBANK/AB073569 GENBANK/AB073570 GENBANK/AB073571 GENBANK/AB073572 GENBANK/AB073573 GENBANK/AB073574 GENBANK/AB073575 GENBANK/AB073576 GENBANK/AB073577 GENBANK/AB073578 GENBANK/AB073579 GENBANK/AB073580 GENBANK/AB073581 GENBANK/AB073582 GENBANK/AB073583 GENBANK/AB073584 GENBANK/AB073585 GENBANK/AB073586 GENBANK/AB073587 GENBANK/AB073588 GENBANK/AB073589 GENBANK/AB073590 GENBANK/AB073591 GENBANK/AB073592 GENBANK/AB073593 GENBANK/AB073594 GENBANK/AB073595 GENBANK/AB073596 Algae/microbiology Algae, Green/*growth & development/microbiology Bacteroides/genetics/growth & development/*isolation & purification Culture Media Cytophaga/genetics/growth & development/*isolation & purification DNA Gyrase/genetics DNA, Ribosomal/analysis Flavobacterium/genetics/growth & development/*isolation & purification Molecular Sequence Data Morphogenesis *Phylogeny RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA
Remarks: Many green algae cannot develop normally when they are grown under axenic conditions. Monostroma oxyspermum, for example, proliferates unicellularly in an aseptic culture, but develops into a normal foliaceous gametophyte in the presence of some marine bacteria. More than 1000 bacterial strains were isolated from marine algae and sponges and assayed for their ability to induce the morphogenesis of unicellular M. oxyspermum. Fifty bacterial strains exhibiting morphogenesis-inducing activity against unicellular M. oxyspermum were isolated. The partial gyrB (approximately 1.2 kbp) and 16S rDNA (approximately 1.4 kbp) sequences of about 40 active strains were determined, and their phylogenetic relationships were analysed. All these strains were located within the Cytophaga-Flavobacterium-Bacteroides (CFB) complex, and most of these strains were clustered in a clade comprising Zobellia uliginosa. On the other hand, these bacteria also exhibited morphogenetic activity against germ-free spores of Ulva pertusa, Ulva conglobata and Enteromorpha intestinalis. Moreover, these bacteria induced the release of spores from the leafy young gametophyte of M. oxyspermum. These results indicate that strains belonging to several groups in the CFB complex play an important role in the normal development of green algae in the marine coastal environment.
URL: 12542710
Ref #: 12216
Author(s): Ruimy,R.;Podglajen,I.;Breuil,J.;Christen,R.;Collatz,E.
Journal: J Bacteriol
Title: A recent fixation of cfiA genes in a monophyletic cluster of Bacteroides fragilis is correlated with the presence of multiple insertion elements
Volume: 178
Page(s): 1914-8
Year: 1996
Keyword(s): GENBANK/X83935 GENBANK/X83936 GENBANK/X83937 GENBANK/X83938 GENBANK/X83939 GENBANK/X83940 GENBANK/X83941 GENBANK/X83942 GENBANK/X83943 GENBANK/X83944 GENBANK/X83945 GENBANK/X83946 GENBANK/X83947 GENBANK/X83948 GENBANK/X83949 GENBANK/X83950 GENBANK/X83951 GENBANK/X83952 GENBANK/X83953 GENBANK/X83954 Bacteroides fragilis/*classification/*genetics Base Sequence Cytophagaceae/classification/genetics *DNA Transposable Elements DNA, Bacterial DNA, Ribosomal Flavobacterium/classification/genetics *Genes, Bacterial Molecular Sequence Data Multigene Family Phylogeny Support, Non-U.S. Gov't beta-Lactamases/classification/*genetics
Remarks: Small-subunit ribosomal DNA sequences of 16 strains of Bacteroides fragilis were determined and compared with previously published sequences. Three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods) as well as a bootstrap analysis were used to assess the robustness of each topology. All phylogenetic analyses demonstrated that the B. fragilis strains were clearly divided into two robust monophyletic units which corresponded to the cfiA-negative and cfiA-positive groups. Strains of two previously identified DNA homology groups separated similarly into the two monophyletic units. According to the intensity of the hybridization signal with a cfiA probe, the cfiA-positive cluster could be further divided into two groups. This difference might reflect the existence of two, probably closely related cfiA-type genes. In the strongly hybridizing cfiA-positive strains, the gene is capable of conferring high-level resistance to the carbapenems and to most beta-lactamase inhibitors as well, while in the weakly hybridizing cfiA-positive strains, only the latter type of resistance is known to occur. The presence of the cfiA-type genes within a monophyletic cluster of B. fragilis that apparently represents only a minority of the species B. fragilis is suggestive of a recent acquisition. The fact that this cluster is also the predominant pool of all known B. fragilis insertion elements, which have been found to play an important role in the expression of carbapenem resistance, raises the possibility that both genetic determinants, i.e., the resistance gene(s) and insertion elements, may have coevolved.
URL: 96178954
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 1394
Author(s): Cato,E.P.;Johnson,J.L.
Journal: Int. J. Syst. Bacteriol.
Title: Reinstatement of species rank for Bacteroides fragilis, B. ovatus, B. distasonis, B. thetajotaomicron, and B. vulgatus: designation of neotype strains for Bacteroides fragilis (Veillon and Zuber) Castellani and Chalmers and Bacteroides thetajotaomicron (
Volume: 26
Page(s): 230-237
Year: 1976
Ref #: 1414
Author(s): Sutter,V.L.;Barry,A.L.;Wilkins,T.D.;Zabransky,R.J.
Journal: Antimicrob. Agents Chemother.
Title: Collaborative evaluation of a proposed reference dilution method of susceptibility testing of anaerobic bacteria.
Volume: 16
Page(s): 495-502
Year: 1979
Ref #: 1415
Title: National Committee for Clinical Laboratory Standards. 1979. Proposed reference dilution procedure for antimicrobic susceptibility testing of anaerobic bacteria. Provisional Standard, PSM-11.
Year: 1979
Ref #: 3968
Author(s): Johnson,J.L.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomy of the bacteroides. I. Deoxyribonucleic acid homologies among Bacteroides fragilis and other saccharolytic Bacteroides species.
Volume: 28
Page(s): 245-256
Year: 1978
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (CN 6300, ATCC 25285) Type strain / L. P. Garrod, St. Bartholomews Hospital, London in 1955 / Appendix abscess / Loesche, W. J. et al. (1964) J. Bact. 88, 1329
Accession Date: 01/01/1955
History: GARROD L P ST BARTHOLMEWS HOSPITAL LONDON
Authority: (Veillon and Zuber 1898) Castellani and Chalmers 1919 (AL)
Depositor: GARROD L P
Taxonomy: TaxLink: S464 (Bacteroides fragilis (Veillon and Zuber 1898) Castellani and Chalmers 1919) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

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