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Bacteria Collection: Mannheimia haemolytica

NCTC Number: NCTC 9380
Current Name: Mannheimia haemolytica
Original Strain Reference: 1266/56 A & B
Other Collection No: ATCC 33396; DSM 10531; 1266/56 A & B
Previous Catalogue Name: Pasteurella haemolytica
Type Strain: Yes
Family: Pasteurellaceae
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar serotype 2
Conditions for growth on solid media: Columbia blood agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1068201
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AF060699|NCTC 9380 T|Mannheimia haemolytica 16S ribosomal RNA gene, partial sequence.| agagtttgatymtgg...
Extended Bibliography: showhide Show bibliography
Ref #: 55571
Author(s): Angen,O.;Mutters,R.;Caugant,D.A.;Olsen,J.E.;Bisgaard,M.
Journal: Int J Syst Bacteriol
Title: Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov
Volume: 49 Pt 1
Page(s): 67-86
Year: 1999
Keyword(s): GENBANK/AF053887 GENBANK/AF053888 GENBANK/AF053889 GENBANK/AF053890 GENBANK/AF053891 GENBANK/AF053892 GENBANK/AF053893 GENBANK/AF053894 GENBANK/AF053895 GENBANK/AF053896 GENBANK/AF053897 GENBANK/AF053898 GENBANK/AF053899 GENBANK/AF053900 GENBANK/AF053901 GENBANK/AF053902 GENBANK/AF060699 Base Composition Base Sequence DNA, Bacterial/*analysis Mannheimia haemolytica/*classification/genetics *Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S/*chemistry
Remarks: The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as 'biotype A' of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov. corresponds to [P.] haemolytica biogroups 3A-3H and the beta-glucosidase and meso-inositol-positive strains of [P.] haemolytica biogroup 9. All typable strains within M. glucosida belong to serotype 11. Mannheimia ruminalis sp. nov. consists of strains previously classified as Bisgaard taxon 18 and [P.] haemolytica biogroup 8D. Finally, Mannheimia varigena sp. nov. includes [P.] haemolytica biogroup 6 as well as Bisgaard taxon 15 and Bisgaard taxon 36. The type strains are NCTC 9380T (M. haemolytica), ATCC 49244T (M. granulomatis), CCUG 38457T = P925T (M. glucosida), CCUG 38470T = HPA92T (M. ruminalis) and CCUG 38462T = 177T (M. varigena).
URL: 10028248
Ref #: 50372
Author(s): Angen,O.;Ahrens,P.;Kuhnert,P.;Christensen,H.;Mutters,R.
Journal: Int J Syst Evol Microbiol
Title: Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis 'Haemophilus somnus', 'Haemophilus agni' and 'Histophilus ovis'
Volume: 53
Page(s): 1449-56
Year: 2003
Keyword(s): GENBANK/AF549387 GENBANK/AF549388 GENBANK/AF549389 GENBANK/AF549390 GENBANK/AF549391 GENBANK/AF549392 GENBANK/AF549393 GENBANK/AF549394 GENBANK/AF549395 GENBANK/AF549396 GENBANK/AF549397 GENBANK/AF549398 GENBANK/AF549399 GENBANK/AF549400 GENBANK/AF549401 GENBANK/AF549402 GENBANK/AF549403 GENBANK/AF549404 GENBANK/AF549405 GENBANK/AY170207 GENBANK/AY170208 GENBANK/AY170209 GENBANK/AY170210 GENBANK/AY170211 GENBANK/AY170212 GENBANK/AY170213 GENBANK/AY170214 GENBANK/AY170215 GENBANK/AY170216 GENBANK/AY170217 GENBANK/AY170218 GENBANK/AY170219 DNA, Bacterial/genetics DNA, Ribosomal/genetics DNA-Directed RNA Polymerases/genetics Genes, Bacterial Haemophilus/*classification/genetics/metabolism Molecular Sequence Data Pasteurellaceae/*classification/genetics/metabolism Phenotype Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Species Specificity
Remarks: Earlier investigations have shown that 'Haemophilus somnus', 'Haemophilus agni' and 'Histophilus ovis' represent the same species. In the present investigation, the taxonomic position of this species is explored further by sequencing the 16S rRNA and rpoB genes of strains that were investigated previously by DNA-DNA hybridization. These results clearly support the allocation of this species to a novel genus within the family PASTEURELLACEAE: The phenotypic separation of Histophilus somni gen. nov., sp. nov. from other members of the family can, for most strains, be based on capnophilia, yellowish pigmentation and indole production. However, due to phenotypic variation, the use of a species-specific PCR test based on the 16S rRNA gene is included in the species description. This is justified by the high sequence similarity of the 16S rRNA gene within the species and the fact that the highest sequence similarity to any other taxon within the family is 93.4 %. The type strain, 8025(T)=ATCC 43625(T)=CCUG 36157(T), was isolated in the USA from a bovine brain with lesions of thromboembolic meningoencephalitis.
URL: 13130032
Ref #: 47767
Author(s): Christensen,H.;Kuhnert,P.;Olsen,J.E.;Bisgaard,M.
Journal: Int J Syst Evol Microbiol
Title: Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae
Volume: 54
Page(s): 1601-9
Year: 2004
Keyword(s): GENBANK/AY296207 GENBANK/AY296208 GENBANK/AY296209 GENBANK/AY296210 GENBANK/AY296211 GENBANK/AY296212 GENBANK/AY296213 GENBANK/AY296214 GENBANK/AY296215 GENBANK/AY296216 GENBANK/AY296217 GENBANK/AY296218 GENBANK/AY296219 GENBANK/AY296220 GENBANK/AY296221 GENBANK/AY296222 GENBANK/AY296223 GENBANK/AY296224 GENBANK/AY296225 GENBANK/AY296226 GENBANK/AY296227 GENBANK/AY296228 GENBANK/AY296229 GENBANK/AY296230 GENBANK/AY296231 GENBANK/AY296232 GENBANK/AY296233 GENBANK/AY296234 GENBANK/AY296235 GENBANK/AY296236 GENBANK/AY296237 GENBANK/AY296238 GENBANK/AY296239 GENBANK/AY296240 GENBANK/AY296241 GENBANK/AY296242 GENBANK/AY296243 GENBANK/AY296244 GENBANK/AY296245 GENBANK/AY296246 GENBANK/AY314025 GENBANK/AY314043 Actinobacillus/genetics Bacterial Proteins/genetics DNA, Bacterial/chemistry/isolation & purification DNA, Ribosomal/chemistry/isolation & purification DNA-Directed RNA Polymerases/*genetics Evolution Genes, Bacterial Genes, rRNA Haemophilus/genetics Mannheimia/genetics Molecular Sequence Data Pasteurella/genetics Pasteurellaceae/*classification/*genetics Phylogeny Prokaryotic Initiation Factor-2/*genetics RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Analysis, DNA Transcription Factors/*genetics
Remarks: Phylogenies of housekeeping gene and 16S rRNA gene sequences were compared to improve the classification of the bacterial family Pasteurellaceae and knowledge of the evolutionary relationships of its members. Deduced partial protein sequences of the housekeeping genes atpD, infB and rpoB were compared in 28, 36 and 28 representative taxa of the Pasteurellaceae, respectively. The monophyly of representatives of the genus Gallibacterium was recognized by analysis of all housekeeping genes, while members of Mannheimia, Actinobacillus sensu stricto and the core group of Pasteurella sensu stricto formed monophyletic groups with two out of three housekeeping genes. Representatives of Mannheimia, Actinobacillus sensu stricto, [Haemophilus] ducreyi and [Pasteurella] trehalosi formed a monophyletic unit by analysis of all three housekeeping genes, which was in contrast to the 16S rRNA gene-derived phylogeny, where these taxa occurred at separate positions in the phylogenetic tree. Representatives of the Rodent, Avian and Aphrophilus-Haemophilus 16S rRNA gene groups were weakly supported by phylogenetic analysis of housekeeping genes. Phylogenies derived by comparison of the housekeeping genes diverged significantly from the 16S rRNA gene-derived phylogeny as evaluated by the likelihood ratio test. A low degree of congruence was also observed between the individual housekeeping gene-derived phylogenies. Estimates on speciation derived from 16S rRNA and housekeeping gene sequence comparisons resulted in quite different evolutionary scenarios for members of the Pasteurellaceae. The phylogeny based on the housekeeping genes supported observed host associations between Mannheimia, Actinobacillus sensu stricto and [Pasteurella] trehalosi and animals with paired hooves.
URL: 15388716
Ref #: 13027
Author(s): Angen,O.;Mutters,R.;Caugant,D.A.;Olsen,J.E.;Bisgaard,M.
Journal: Int J Syst Bacteriol
Title: Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov
Volume: 49 Pt 1
Page(s): 67-86
Year: 1999
Keyword(s): GENBANK/AF053887 GENBANK/AF053888 GENBANK/AF053889 GENBANK/AF053890 GENBANK/AF053891 GENBANK/AF053892 GENBANK/AF053893 GENBANK/AF053894 GENBANK/AF053895 GENBANK/AF053896 GENBANK/AF053897 GENBANK/AF053898 GENBANK/AF053899 GENBANK/AF053900 GENBANK/AF053901 GENBANK/AF053902 GENBANK/AF060699 Base Composition Base Sequence DNA, Bacterial/*analysis Mannheimia haemolytica/*classification/genetics *Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S/*chemistry Support, Non-U.S. Gov't
Remarks: The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as 'biotype A' of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov. corresponds to [P.] haemolytica biogroups 3A-3H and the beta-glucosidase and meso-inositol-positive strains of [P.] haemolytica biogroup 9. All typable strains within M. glucosida belong to serotype 11. Mannheimia ruminalis sp. nov. consists of strains previously classified as Bisgaard taxon 18 and [P.] haemolytica biogroup 8D. Finally, Mannheimia varigena sp. nov. includes [P.] haemolytica biogroup 6 as well as Bisgaard taxon 15 and Bisgaard taxon 36. The type strains are NCTC 9380T (M. haemolytica), ATCC 49244T (M. granulomatis), CCUG 38457T = P925T (M. glucosida), CCUG 38470T = HPA92T (M. ruminalis) and CCUG 38462T = 177T (M. varigena).
URL: 99152574
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 6425
Author(s): Newsome,I.E.;Cross,F.
Journal: J. Am. Vet. Med. Assoc.
Title: Some bipolar organisms found in pneumonia in sheep.
Volume: 80
Page(s): 711-719
Year: 1932
Ref #: 7016
Author(s): Busse,H.-J.;Bunka,S.;Hensel,A.;Lubitz,W.
Journal: Int. J. Syst. Bacteriol.
Title: Discrimination of members of the family Pasteurellaceae based on polyamine patterns.
Volume: 47
Page(s): 698-708
Year: 1997
Ref #: 7308
Author(s): Angen,O.;Mutters,R.;Caugant,D.A.;Olsen,J.E.;Bisgaard,M.
Journal: Int. J. Syst. Bacteriol.
Title: Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. no
Volume: 49
Page(s): 67-86
Year: 1999
Data: Type strain / J. A. Watt, Edinburgh in 1956 / Biotype A, Serotype 2
Accession Date: 01/01/1956
Authority: (Newsome and Cross 1932) Angen et al. 1999
Depositor: WATT J A
Taxonomy: TaxLink: S2221 (Pasteurella haemolytica) - Date of change: 5/02/2003
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