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Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Aggregatibacter actinomycetemcomitans

NCTC Number: NCTC 9710
Current Name: Aggregatibacter actinomycetemcomitans
Original Strain Reference: 277/49-50 (8)
Other Collection No: ATCC 33384; CCUG 13227; DSM 8324
Previous Catalogue Name: Haemophilus actinomycetemcomitans
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 48 hours, 37C, CO2
Isolated From: abscess
16S rRNA Gene Sequence: U07777|ATCC 33384|Actinobacillus actinomycetemcomitans ATCC 33384 16S rRNA gene and16S-23S spacer, partial sequence, and tRNA-Glu gene, completesequence.| acctccttaccaata...
Bibliography: HOLM P 1954 ACTA PATH MICROBIOL SCAND 34 235
Extended Bibliography: showhide Show bibliography
Ref #: 95543
Author(s): Leys,E.J.;Griffen,A.L.;Strong,S.J.;Fuerst,P.A.
Journal: J Clin Microbiol
Title: Detection and strain identification of Actinobacillus actinomycetemcomitans by nested PCR
Volume: 32
Page(s): 1288-94
Year: 1994
Keyword(s): GENBANK/U07776 GENBANK/U07777 GENBANK/U07778 Actinobacillus actinomycetemcomitans/classification/*genetics/*isolation & purification Adolescent Adult Aged Base Sequence Child Child, Preschool DNA Primers/genetics DNA, Bacterial/genetics DNA, Ribosomal/genetics Dental Plaque/microbiology Humans Middle Aged Molecular Sequence Data Phylogeny Polymerase Chain Reaction/*methods/statistics & numerical data Porphyromonas gingivalis/genetics/isolation & purification Sensitivity and Specificity Sequence Homology, Nucleic Acid Serotyping Species Specificity
Remarks: By using PCR, Actinobacillus actinomycetemcomitans strains were identified directly from plaque samples without the need to isolate or culture bacteria. DNA fragments were generated by a nested, two-step PCR amplification of the ribosomal spacer region between the 16S and 23S rRNA genes. For the first amplification, primers homologous to sequences common to all bacterial species were used. This was followed by a second amplification with primers specific to A. actinomycetemcomitans. The ribosomal DNA spacer region was amplified from as few as 10 bacterial cells within a total population of 10(8) cells (0.00001%), and cross-reactivity between species was not observed. DNA fragments specific for Porphyromonas gingivalis were generated from the same samples by using a P. gingivalis-specific primer, and equivalent sensitivity and specificity were observed. A. actinomycetemcomitans was detected in 60% and P. gingivalis was detected in 79% of 52 subjects tested. Sequence analysis of the spacer region DNA fragment for A. actinomycetemcomitans gave precise strain identification, producing unique sequences for seven reference strains and identification of nine plaque-derived isolates. A phylogenetic tree based on quantitative sequence relationships was constructed. Two-step PCR amplification directly from plaque samples combined with sequence analysis of the ribosomal DNA spacer region provides a sensitive assay for detection and strain identification of multiple species directly from a single plaque sample. This simplified approach provides a practical method for large-scale studies on the transmission and pathogenicity of periodontitis-associated bacteria.
URL: 8051258
Ref #: 4905
Author(s): Potts,T.V.;Zambon,J.J.;Genco,R.J.
Journal: Int. J. Syst. Bacteriol.
Title: Reassignment of Actinobacillus actinomycetemcomitans to the genus Haemophilus as Haemophilus actinomycetemcomitans comb. nov.
Volume: 35
Page(s): 337-341
Year: 1985
Ref #: 4906
Author(s): Brondz,I.;Olsen,I.
Journal: Int. J. Syst. Bacteriol.
Title: Multivariate analyses of carbohydrate data from lipopolysaccharides of Actinobacillus (Haemophilus) actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus.
Volume: 40
Page(s): 405-408
Year: 1990
Ref #: 4907
Author(s): DeLey,J.;Mannheim,W.;Mutters,R.;Piechulla,K.;Tytgat,R.;Segers,P.;Bisgaard,M.;Frederiksen,W.;Hinz,K.-H.;Vanhoucke,M.
Journal: Int. J. Syst. Bacteriol.
Title: Inter- and intrafamilial similarities of rRNA cistrons of the Pasteurellaceae.
Volume: 40
Page(s): 126-137
Year: 1990
Ref #: 7016
Author(s): Busse,H.-J.;Bunka,S.;Hensel,A.;Lubitz,W.
Journal: Int. J. Syst. Bacteriol.
Title: Discrimination of members of the family Pasteurellaceae based on polyamine patterns.
Volume: 47
Page(s): 698-708
Year: 1997
Data: Type strain / P. Holm, Copenhagen in 1955 / Abscess in 1949 / Holm, P. (1954) Acta path. Microbiol. Scand. 34, 235
Accession Date: 01/01/1955
History: STATE SERUM INSTITUTE,COPENHAGEN
Authority: (Klinger 1912) Nørskov-Lauritsen & Kilian 2006 (VP)
Depositor: HOLM P
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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