Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety

Bacteria Collection: Actinomyces odontolyticus

NCTC Number: NCTC 9935
Current Name: Actinomyces odontolyticus
Original Strain Reference: A 10
Other Collection No: ATCC 17929; A 10
Previous Catalogue Name: Actinomyces odontolyticus
Type Strain: Yes
Family: Actinomycetaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 48-72 hours, 37°C, anaerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: dental caries
16S rRNA Gene Sequence: >gb|X80504|NCTC 9935|A.odontolyticus 16S rDNA.| cgggcgtgcttaaca... >gb|AF058053|NCTC 9935|Actinomyces odontolyticus 16S ribosomal RNA gene, partial sequence.| cgccggaaacgggtg...
Bibliography: BATTY I 1958 J PATH BACT 75 455
Extended Bibliography: showhide Show bibliography
Ref #: 16452
Author(s): Jauh-Hsun,C.;Vinh,T.;Davies,J.K.;Figdor,D.
Journal: Oral Microbiol Immunol
Title: Molecular approaches to the differentiation of Actinomyces species
Volume: 14
Page(s): 250-6
Year: 1999
Keyword(s): Actinomyces/*classification/*genetics Bacterial Typing Techniques DNA Probes DNA, Bacterial/analysis Oligonucleotide Probes RNA, Ribosomal, 16S/analysis Sequence Analysis, DNA Sequence Analysis, RNA Species Specificity
Remarks: The definition of the genus Actinomyces relies heavily on traditional methods of taxonomy. This study sought to develop molecular tools for the identification of strains of Actinomyces israelii and Actinomyces gerencseriae. Oligonucleotide probes were designed and one of these successfully differentiated. A. gerencseriae from ten strains of A. israelii and three other Actinomyces species by DNA:DNA hybridization. However, probes based on known 16S rRNA sequences failed to hybridize to all the strains previously identified as A. israelii. Using the PCR technique, a region encoding a portion of the 16S rRNA was amplified from genomic DNA. The results showed that A. israelii can be divided into three different groups based on comparison of the amplified DNA sequences. This information should allow the development of probes that are specific for these newly identified groups of strains within the species A. israelii.
URL: 10551170
Data: (ATCC 17929) Type strain / I. Batty, Birmingham in 1956 / Dental caries / Batty, I. (1958) J. path. Bact. 75, 455
Accession Date: 01/01/1956
Authority: Batty 1958 (AL)
Depositor: BATTY I
Taxonomy: TaxLink: S138 (Actinomyces odontolyticus Batty 1958) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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