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UK Health Security Agency

ANGM-CSS

ANGM-CSS

Catalogue No.

08040401

Cell Line Name

ANGM-CSS

Cell Line Description

A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)

Characteristics

Tissue of Origin

Brain

Morphology

Fusiform, can form heaps of cells

Karyotype

Near tetraploid; range 88 to 91 - see Cell Line Description for more information

Disease

Glioblastoma

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂;37ºC.

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Not Specified

Additional Info

Depositor

Dr A Notarangelo, IRCCS-CASA, Sollievo Della Sofferenza Hospital, Viale Cappuccini 71013, San Giovanni Rotondo (Fg), Italy

Country of Origin

Italy

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Notarangelo A1, Trombetta D2, D'Angelo V3, Parrella P2, Palumbo O1, Storlazzi CT4, Impera L4, Muscarella LA2, La Torre A2, Affuso A5, Fazio VM2, Carella M1, Zelante L1.2014 Establishment and genetic characterization of ANGM-CSS, a novel, immortal cell line derived from a human glioblastoma multiforme. Int J Oncol. 44(3):717-24. PMID: 24366606.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: ANGM-CSS (ECACC 08040401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.