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UK Health Security Agency

NH15-CA2

NH15-CA2

Catalogue No.

08062525

Cell Line Name

NH15-CA2

Cell Line Description

NH15-CA2 is a hybrid cell line formed by the fusion of N115-BU-8 (a bromodeoxyuridine-resistant mutant of N1E-115 (ECACC catalogue number 08062519) and 108CC15 (ECACC catalogue number 08062516) (itself a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid line). This “hybrid-hybrid” line contains both cholinergic and adrenergic enzyme activities.

Characteristics

Products

Acetylcholine and noradrenaline

Tissue of Origin

Brain

Applications

used as a neuronal model

Disease

Neuroblastoma

Culture Conditions

Cell Type

Neuronal

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 µM aminopterin and 16 µM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days

Growth Mode

Adherent

Additional Info

Depositor

Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.

Country of Origin

Germany

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Heumann et al., (1979) Clonal hybrid cell lines expressing cholinergic and adrenergic properties. Proc. Natl. Acad. Sci. 76: 4674-4677.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: NH15-CA2 (ECACC 08062525).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.