|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||MKL-1|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: MKL-1 (ECACC 09111801)|
|Keywords:||Human neuroendocrine skin carcinoma|
|Cell Line Description:||MKL-1 was established from a nodal metastasis in a 26-year-old Caucasian male patient. MKL-1 cells contain a Merkel cell polyomavirus integrated into the genome. MKL-1 cells cause tumour formation at 4-6 weeks at subcutaneous sites in athymic male nude mice (BALB/c nu/nu).|
|Tissue of Origin:||Skin|
|CellType:||Loosely packed floating aggregates with irregular outline and no central necrosis|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Seed at 3 x 105 cells/ml in 5% CO2; 37°C Saturation density is approximately 106 cells/ml. Population doubling time is around 120 hours.|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 10% FBS|
|Depositor:||Dr Patrick Moore, University of Pittsburgh Cancer Institute, 5117 Centre Avenue Suite 1.8 Pittsburgh PA 15213 (Originator: Dr Victor Gould & Dr James Radosevich, Department of Pathology, Rush Medical College, 1753 west Congress Parkway, Chicago, Illinois, 60612)|
|References:||Rosen et al., (1987) Establishment and characterization of a neuroendocrine skin carcinoma cell line. Laboratory Investigation 56: 302-312 PMID: 3546933.|
|Additional Bibliography:||Feng et al., (2008) Clonal integration of a polyomavirus in human Merkel cell carcinoma. Science 319: 1096-1100. PMID: 18202256. Shuda et al., (2008) T antigen mutations are a human tumor-specific signature for Merkel cell polyomavirus. Proc. Natl. Acad. Sci. USA 105: 16063-4 PMID: 18812503. Houben R, Shuda M, Weinkam R, Schrama D, Feng H, Chang Y, Moore PS, Becker JC.(2010) Merkel cell polyomavirus-infected Merkel cell carcinoma cells require expression of viral T antigens. J Virol. 84(14):7064-72. PMID: 20444890.|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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