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NO36

NO36

Catalogue No.

10092312

Cell Line Name

NO36

Cell Line Description

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. NO36 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are thick and stellate shaped with vacuoles. This cell line is distributed by ECACC on behalf of CellBank Australia, Children's Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by CellBank Australia. CellBank Australia reference number: CBA-0142.

Characteristics

Tissue of Origin

Skin

Morphology

Thick stellate shaped cells with vacuoles

Applications

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods

Disease

Mesothelioma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Optimal split ratio 1:8 (seeding density 10⁴ cells/cm²).Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Culture Medium

RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

Growth Mode

Adherent

Additional Info

Depositor

Richard Lake - University of Western Australia, Australia

Country of Origin

Australia

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Manning LS, Whitaker D, Murch AR, Garlepp MJ, Davis MR, Musk AW, Robinson BW (1991) Int J Cancer Jan 21;47(2):285-90

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: NO36 (ECACC 10092312).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.