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P4E6

P4E6

Catalogue No.

10112301

Cell Line Name

P4E6

Cell Line Description

P4E6 is an Immortalised human prostate cell line derived from a biopsy of a well-differentiated early stage prostate cancer (Gleason grade (4)) P4E6 was immortalised using human papillomavirus type E6 gene from PLXSN16E6 retroviral stock (Denise Galloway, Fred Hutchinson Cancer Centre Seattle). The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

General Info

Species

Human

Release Conditions

Restricted - all organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

Not specified

Products

Stains positive for pancytokeratin, cytokeratin 8, vimentin (weak) and chromogranin A. Prostate markers expressed include prostate specific antigen (PSA) prostate specific membrane antigen (PSMA), negative for expression of androgen receptor (AR).

Tissue of Origin

Prostate gland

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12
D5S818: 12
D7S820: 7,8
D13S317: 11
D16S539: 11
TH01: 7,9.3
TPOX: 8
vWA: 17,19

Karyotype

Not specified

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Culture Medium

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.

Growth Mode

Adherent

Additional Info

Depositor

Professor. Norman J Maitland Director YCR Cancer Research Unit Department of Biology (Area 13) University of York Heslington York YO10 5DD

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Maitland NJ, Macintosh CA, Hall J, Sharrard M, Quinn G and Lang S. (2001) In vitro models to study cellular differentiation and function in human prostate cancers. Radiation Research 155, 133-142. PMID: 11121225

Bibliography

Lang SH, et.al; (2006) Differentiation of prostate epithelial cell cultures by matrigel/stromal cell glandular reconstruction. In Vitro Cell Dev Biol Anim.42:273-80 PMID: 17163777. Maitland NJ, Macintosh CA, Schmitz C and Lang S. (2004) Immortalisation of human prostate cells with the human papillomavirus type 16 E6 gene. Methods In Molecular Medicine 88: 275-285 PMID: 14634239. Lang SH, et.al; (2001) Enhanced expression of vimentin in motile prostate cell lines and in poorly differentiated and metastatic prostate carcinoma. Prostate 52: 253-263 PMID: 12210485.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: P4E6 (ECACC 10112301).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.