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SW1222

SW1222

Catalogue No.

12022910

Cell Line Name

SW1222

Cell Line Description

The SW1222 cell line was established from a patient with a moderately well differentiated adenocarcinoma of the colon classified as Dukes' stage C. SW1222 cells produce tumours in female athymic nu/nu nude mice.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Colon

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11
D5S818: 9
D7S820: 10,12
D13S317: 9,13
D16S539: 9,10
TH01: 7
TPOX: 10
vWA: 18

Karyotype

43 chromosomes per metaphase

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine

Growth Mode

Adherent

Additional Info

Depositor

Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD. Originator: Dr A Leibovitz deposited by agreement with Dr Meenhard Herlyn Director of the Wistar Institute, 3601 Spruce St Philadelphia PA 19104

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Richman PI, Bodmer WF. 1988 Control of differentiation in human colorectal carcinoma cell lines: epithelial-mesenchymal interactions. J Pathol.156(3):197-211 PMID: 3204451. Browning MJ, Krausa P, Rowan A, Hill AB, Bicknell DC, Bodmer JG, Bodmer WF.1993 Loss of human leukocyte antigen expression on colorectal tumor cell lines: implications for anti-tumor immunity and immunotherapy. J Immunother Emphasis Tumor Immunol. 14(3):163-8. PMID: 8297898.

Bibliography

Efstathiou JA, Liu D, Wheeler JM, Kim HC, Beck NE, Ilyas M, Karayiannakis AJ, Mortensen NJ, Kmiot W, Playford RJ, Pignatelli M, Bodmer WF.1999 Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639. Rowan AJ, Lamlum H, Ilyas M, Wheeler J, Straub J, Papadopoulou A, Bicknell D, Bodmer WF, Tomlinson IP. 2000 APC mutations in sporadic colorectal tumors: A mutational "hotspot" and interdependence of the "two hits". Proc Natl Acad Sci U S A; 97(7):3352-7. PMID: 10737795. Bracht K, Nicholls AM, Liu Y, Bodmer WF. 2010 5-Fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency. Br J Cancer. 103(3):340-6. PMID: 20606684.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: SW1222 (ECACC 12022910).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.