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CAT-MT

CAT-MT

Catalogue No.

12122001

Cell Line Name

CAT-MT

Cell Line Description

CAT-MT is a feline mammary carcinoma cell line derived from a highly malignant tubular adenocarcinoma. CAT-MT cells are tumourigenic in athymic mice.

Characteristics

Tissue of Origin

Mammary gland

Morphology

Mixed population of polygonal cells

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) seed new cultures at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Professor RW Else, University of Edinburgh, Royal (Dick) School of Veterinary Studies, Easter Bush Veterinary Centre, Easter Bush, Midlothian, EH25 9RG.

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Norval M, Maingay J, Else RW 1985 Characteristics of a feline mammary carcinoma cell line. Res Vet Sci. 1985 39(2):157-64.PMID: 3906801.

Bibliography

Penzo C, Ross M, Muirhead R, Else R, Argyle DJ 2009 Effect of recombinant feline interferon-omega alone and in combination with chemotherapeutic agents on putative tumour-initiating cells and daughter cells derived from canine and feline mammary tumours. Vet Comp Oncol. 7(4):222-9. PMID: 19891692 Penzo C, Ross M, Muirhead R, Else R, Argyle DJ. 2009 Effect of recombinant feline interferon-omega alone and in combination with chemotherapeutic agents on putative tumour-initiating cells and daughter cells derived from canine and feline mammary tumours.Vet Comp Oncol.;7(4):222-9. PMID: 19891692.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: CAT-MT (ECACC 12122001).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.