|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||MCF7 AREc32|
|Organisation deposited by:||Ximbio (formerly known as Cancer Research Technologies Limited)|
|Other Collection No.:||No|
|Unique to ECACC:||Yes|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: MCF7 AREc32 (ECACC 16071902)|
|Keywords:||Cancer; drug metabolism, Antioxidant Response Element (ARE)- Luciferase|
|Cell Line Description:||
The stable human mammary MCF7-derived reporter cell line called AREc32 contains copies of the rat Glutathione-S-transferase (GST) antioxidant response element (ARE) linked to a luciferase gene, such that the induction of the ARE results in luciferase activity.
ARE is a transcriptional regulatory element involved in the activation of genes coding for a number of antioxidant proteins and enzymes that work in concert to protect tissues from oxidative insults. This cell line can be used to investigate whether anticancer drugs can induce ARE-driven gene expression.
|Tissue of Origin:||Breast|
DNA Profile (STR Analysis):
Amelogenin: X X
CSF1PO: 10, 10
D16S539: 11, 12
D21S11: 30, 30
D5S818: 11, 12
D8S1178: 10, 14
FGA: 23, 25
Penta D: 12, 12
Penta E: 7, 12
THO1: 6, 6
TPOX: 9, 12
|Genetic Modification:||Yes. The ARE-luciferase reporter plasmid was generated using the pGL3-promoter vector containing an SV40 promoter upstream of the firefly luciferase gene.|
|Hazard Group (ACDP):||2|
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
Hyperlinks to Material Safety Data Sheets (MSDS) documents:
Frozen cell cultures MSDS
Growing cell cultures MSDS
Nucleic acids derived from cell cultures MSDS
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x104 cells/cm2using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO2, and passaged every 3 to 4 days|
|Culture Medium:||Dulbecco's Moodifies Eagle Medium (DMEM) with 2.5 mM Glutamax supplemented with 10% foetal bovine serum. Do not culture beyond 15 passages after revival|
|Depositor:||Ximbio (formerly known as Cancer Research Technologies Limited)|
Wang XJ, Hayes JD, Henderson CJ, Wolf CR. Identification of retinoic acid as an inhibitor of transcription factor Nrf2 through activation of retinoic acid receptor alpha. Proc Natl Acad Sci U S A. 2007 Dec 4; 104(49):19589-94. Epub 2007 Nov 28. PubMed PMID: 18048326; PubMed Central PMCID: PMC2148333.
Wang XJ, Hayes JD, Wolf CR. Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of nrf2 by cancer chemotherapeutic agents. Cancer Res. 2006 Nov 15;66 (22):10983-94. PubMed PMID: 17108137.
Escher BI, Dutt M, Maylin E, Tang JY, Toze S, Wolf CR, Lang M. Water quality assessment using the AREc32 reporter gene assay indicative of the oxidative stress response pathway. J Environ Monit. 2012 Nov;14(11):2877-85. doi: 10.1039/c2em30506b.
Buendia I, Navarro E, Michalska P, Gameiro I, Egea J, Abril S, López A, González-Lafuente L, López MG, León R. New melatonin-cinnamate hybrids as multi-target drugs for neurodegenerative diseases: Nrf2-induction, antioxidant effect and neuroprotection. Future Med Chem. 2015;7(15):1961-9. doi: 10.4155/fmc.15.99. Epub 2015 Oct 23.
|Release Conditions:||For research use only. Commercial organisations are required to fill in a ‘Cell line release authorisation for research use in commercial organisations’ form.|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Please confirm your country of origin from the list below.