|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||B95-8|
|Other Collection No.:||ATCC CRL 1612|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: B95-8 (ECACC 85011419)|
|Keywords:||Cotton-top Tamarin Monkey peripheral blood lymphocyte|
|Cell Line Description:||
Derived from a cotton-top tamarin (Saguinus oedipus). Releases high titres of transforming EBV. Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells should be handled under laboratory containment level 2.
In some instances the cell line B95-8 has been described as derived from ‘marmoset’, however, this is not correct. B95-8 was derived from a cotton-top tamarin (Saguinas oedipus); this has been confirmed by DNA profiling.
|Tissue of Origin:||blood|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Maintain cultures between 3-9x100,000 cells/ml. May need to use 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended that this cell line be frozen at no less than 8x1000,000 cells per ampoule.|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford|
|References:||Proc Natl Acad Sci, USA 1973;70:190|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
|Release Conditions:||Yes - CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the United Kingdom|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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