|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||3T3 Swiss Albino|
|Other Collection No.:||ATCC CCL 92|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: 3T3 Swiss Albino (ECACC 85022108)|
|Keywords:||Mouse Swiss Albino embryo fibroblast|
|Cell Line Description:||
Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.
Contact inhibition is the natural process of arresting cell growth when cells come into contact with each other. It is not possible to be sure how the loss of contact inhibition may affect other characteristics of the cell line.
This cell line catalogue number continues to be a popular choice. The key consideration is whether the characteristic of contact inhibition is required for the work the cell line is to be used for. For example, it is required when testing the affects of expression of potential oncogenes where the induction of the loss of contact inhibition is being measured, whereas it is of less significance when using the cell line to express recombinant proteins for production and purification.
|Tissue of Origin:||embryo|
|Karyotype:||2n = 40; hypertriploid|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.|
|Culture Medium:||DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford|
|References:||Virology 1965;27:179; J Cell Biol 1963;17:299; PNAS USA 1964;51:66|
|Additional Bibliography:||Not specified|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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