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Y3.AG.1.2.3

Y3.AG.1.2.3

Catalogue No.

85110502

Cell Line Name

Y3.AG.1.2.3

Cell Line Description

Sub-cloned from an azaguanine-resistant mutant of the Lou rat myeloma, S210. Cells are HAT sensitive. The cells secrete IgG kappa light chains. Y3.AG.1.2.3 can be used as a fusion partner for rat B cells to make rat-rat hybridomas.

General Info

Species

Rat Lou strain

Other Collection No.

ATCC CRL 1631

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Products

Immunoglobulin G (IgG) kappa light chains

Tissue of Origin

Myeloma

Karyotype

Not specified

Applications

Tumourigenicity studies

Disease

Myeloma

Culture Conditions

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr J Jarvis, MRC Laboratory of Molecular Biology, Cambridge

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Galfre G, Milstein C, Wright B. 1979 Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG. Nature. 277(5692):131-3. PMID: 310519.

Bibliography

Bazin H, Deckers C, Beckers A, Heremans JF.1972 Transplantable immunoglobulin-secreting tumours in rats. I. General features of LOU-Ws1 strain rat immunocytomas and their monoclonal proteins. Int J Cancer.10(3):568-80. PMID: 4668496.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Y3.AG.1.2.3 (ECACC 85110502).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.