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A673

A673

Catalogue No.

85111504

Cell Line Name

A673

Cell Line Description

Derived from a 15 year old female patient. The cells form colonies on soft agar and tumours in antithymocyte serum treated mice.The cell line A673 was originally described in 1973 as derived from a rhabdomyosarcoma, however, more recent analysis of the cells using advanced diagnostic techniques determined the cells to have characteristics of Ewing sarcoma rather than rhabdomyosarcoma. The following references provide more information about this tumour status: Martínez-Ramírez et al. 2003. Characterization of the A673 cell line (Ewing tumor) by molecular cytogenetic techniques. Cancer Genet Cytogenet. Mar;141(2):138-42. PMID:12606131Coleman and Roberts, 2004. Re: Characterization of the A673 cell line (Ewing tumor) by molecular cytogenetic techniques. Cancer Genet Cytogenet. Jan 1;148(1):86. PMID: 14697648Teicher et al., 2011. Characteristics of human Ewing/PNET sarcoma models. Ann Saudi Med. Mar-Apr; 31(2): 174–182. PMCID: PMC3102479

Characteristics

Products

Transforming growth factor (Tramnsforming Growth Factor (TGF)) - ß

Tissue of Origin

Muscle

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 11,12
D7S820: 10,12
D13S317: 8,13
D16S539: 11
TH01: 9.3
TPOX: 8
vWA: 15,18

Karyotype

Near Diploid

Applications

Tumourigenicity studies

Disease

Sarcoma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Nat Cancer Inst 1973;51:1417. PMID: 4357758; Am J Pathol 1993;142:49. PMID: 8380955; Cancer Genet Cytogenet 2003 Mar;141(2):138-42. PMID: 12606131; Cancer Genet Cytogenet. 2004 Jan 1;148(1):86. PMID: 14697648; Ann Saudi Med. 2011 Mar-Apr; 31(2): 174-182. PMCID: PMC3102479

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: A673 (ECACC 85111504).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.