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J45.01

J45.01

Catalogue No.

93031145

Cell Line Name

J45.01

Cell Line Description

Derived from Jurkat E6-1 clone (ECACC catalogue no. 88042803) after gamma irradiation and complement-mediated lysis with anti CD45. Low expression of CD45 (5-8% compared to parent line) results in defective signal transduction through TcR and the accessory molecule, CD2. Interleukin 2 (IL-2) is produced in response to calcium ionophore and phorbol ester stimulation, but it does not produce IL-2 in response to TcR or CD2 stimulation.

Characteristics

Receptors

TcR, CD2, CD28, CD45 (low)

Products

Interleukin 2 (IL-2) in response to calcium ionophores and phorbol ester

Tissue of Origin

Blood

Karyotype

Not specified

Disease

Lymphoblastic Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Proc Natl Acad Sci, USA 1991;88:2037

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: J45.01 (ECACC 93031145).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.