|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||EPC|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: EPC (ECACC 93120820)|
|Keywords:||Fish epithelioma Fathead minnow Pimephales promelas|
|Cell Line Description:||This cell line known as Epithelioma papulosum cyprini (EPC) was originally reported to be from Carp (Cyprinus carpio) epidermal herpes virus-induced hyperplastic lesions. More recently EPC was found to be derived from Fathead Minnow (Pimephales promelas) see Winton et al., 2010. This has been confirmed by DNA sequencing at ECACC. The cells support the growth of pancreatic necrosis virus and rhabdovirus.|
|Tissue of Origin:||Skin|
|Karyotype:||2n = 96|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO2; 20°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.|
|Culture Medium:||EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Dr M Ferrari, Instituto Zooprofilattico, Brescia|
|References:||Fijan N et al., 1983 Some properties of the epithelioma papulosum cyprini (EPC) cell line from Carp Cyprinus carpio. Ann. Virol. (Inst. Pasteur) 134E 207-220.|
|Additional Bibliography:||Winton J, Batts W, deKinkelin P, LeBerre M, Bremont M, Fijan N 2010 Current lineages of the epithelioma papulosum cyprini (EPC) cell line are contaminated with fathead minnow, Pimephales promelas, cells. J Fish Dis. 33(8):701-4 PMID: 20497291.|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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