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UK Health Security Agency

H2.35

H2.35

Catalogue No.

94050407

Cell Line Name

H2.35

Cell Line Description

From a primary hepatocyte culture of 6 week old female BALB/c mouse transformed with tsA255 (temperature-sensitive mutant of SV40 virus). H2.35 is temperature sensitive for expression of serum albumin mRNA. During routine propagation at 33°C it exhibits extremely low levels of albumin transcription and mRNA, while at the restructure temp of 39°C albumin mRNA increases 100 fold if the cells are grown in serum free medium on a collagen substratum. Undergoes morphological changes in response to extracellular matrix gel substrata, albumin gene transcription is induced. Liver-enriched transcription factors are induced in serum-free medium and on collagen gels. The cells should be handled under laboratory containment level 2.

Characteristics

Tissue of Origin

Liver

Karyotype

Not specified

Applications

Provides modal system for studying hepatocyte differentiation because some hepatocyte phenotypes are inducible in the cells. Transfection recipient for assays of genetic regulatory sequence function for liver genes that do not function in other cells.

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 33°C.

Culture Medium

DMEM (containing 1g / l glucose) + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 4% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Proc Natl Acad Sci, USA 1988;85:9076; Mol.Cell.Biol 1990;10:4582, Mol.Cell.Biol 1990;11:773, Mol.Cell.Biol. 1991;11:4405

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: H2.35 (ECACC 94050407).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.