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RPMI 7932

RPMI 7932

Catalogue No.

94072246

Cell Line Name

RPMI 7932

Cell Line Description

Derived from the pleural effusion of a 36 year old female with malignant melanoma. Cell line produces plasminogen activator. This cell line was found to be indistinguishable from cell lines COLO 699N & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.

Characteristics

Products

Plasminogen activator

Tissue of Origin

Pleural effusion

Karyotype

Not specified

Applications

Tumourigenicity studies

Disease

Melanoma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None specified by depositor

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: RPMI 7932 (ECACC 94072246).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.