Catalogue No.
95122021
SSE-5
SSE-5
Cell Line Name
SSE-5
Cell Line Description
SSE-5 was initiated in 1964 from pooled embryos of Sockeye Salmon (Oncorynchus nerka). The cell line that is also known as Sockeye Salmon embryo-5, is susceptible to infectious pancreatic necrosis virus (IPNV), viral hemorrhagic septicaemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV). For replication of salmonid viruses SSE-5 cells should be grown at 18°C.
General Info
Species
Fish
Links
Cellosaurus: CVCL_4305
Release Conditions
Characteristics
Tissue of Origin
Embryo
Karyotype
Modal no. 55
Applications
Isolation of viruses from clinical samples, virus propagation
Disease
None Stated
Culture Conditions
Cell Type
Epithelial-like
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.
Culture Medium
MEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Growth Mode
Adherent
Additional Info
Depositor
Dr J L Fryer, Department of Microbiology, Oregon State University, Corvallis, USA
Country of Origin
United States
Hazard Group (ACDP)
Hazard Group (ACDP) 2
Applications
References
In Vitro 1984; 20:671
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: SSE-5 (ECACC 95122021).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.